The SMARTChip Stroke Study
关键词
抽象
描述
Stroke patients will be prospectively recruited to the trial on admission to A&E. The patient will undergo normal clinical assessment and treatment for stroke. As soon after admission as possible, a finger-prick blood sample will be taken from the patient and the SMARTChip device will be used to measure the levels of purines in the sample. A medical history of factors relevant to the purine test will also be established. This will include factors such as any medications that might alter purine levels, a prior history of strokes, diabetes, a history (individual or family) of gout, and when they last had food. The patient will also be given a CT head scan as part of their normal treatment.
In sub-set of patients repeated blood samples (once per hour for 6 hours) will be taken to gain information on how purine levels may change in blood following a stroke. In all patients, the blood purines will be re-tested at 24h (±3h).
All patients will be offered the option of an MRI brain scan at 24-72h post-admission and recruitment will continue until 100 patients with MRI measurements have been enrolled. The flexibility in the timing of the scan is to allow scheduling of the scan to fit in with other demands on the MRI. The purpose of the scan is to identify the nature of the cerebrovascular event and quantify any infarction or area of brain bleeding.
Control participants will give blood on 2 occasions 24h apart for a purine test. The purpose of this control is two-fold: 1) to establish the variability of blood purines in healthy controls and 2) to test whether stroke patients have elevated blood purine levels compared to healthy controls.
Healthy volunteer feeding controls will give a baseline blood sample, eat a simple purine rich meal (a meat sandwich) and then give 4 more blood samples at 10, 30, 60 and 120 minutes following the meal. The purpose of this control is to test whether ingestion of a recent meal might alter baseline blood purine measurements.
Main study patients recruited at University Hospitals North Midlands NHS Trust will also be given the option of participating in the Spasticity and Pharmacokinetic sub-studies. Participants taking part in the Spasticity sub-study will provide an additional blood sample to measure biomarkers and will undergo a spasticity assessment at admission, 24h, 72h, 7d and one or two clinic visits between 30-90d post-admission. Participants taking part in the pharmacokinetic sub-group will provide an additional blood sample for measurement of glutamate and other biomarkers at admission, 24h, 72h, 7d and 30-90d post-admission.
日期
| 最后验证: | 08/31/2019 |
| 首次提交: | 06/05/2016 |
| 提交的预估入学人数: | 06/08/2016 |
| 首次发布: | 06/09/2016 |
| 上次提交的更新: | 09/25/2019 |
| 最近更新发布: | 09/26/2019 |
| 实际学习开始日期: | 06/30/2016 |
| 预计主要完成日期: | 12/30/2019 |
| 预计完成日期: | 12/30/2019 |
状况或疾病
相
手臂组
| 臂 | 干预/治疗 |
|---|---|
| Stroke patients Adult stroke patients with suspected stroke will be recruited on admission to hospital. Recruitment will continue until 100 patients have received an MRI at 24h-72h post admission, up to a maximum recruitment threshold of 300 patients. | |
| Control patients 50 adult control patients will be recruited from the non-vascular, non-oncological surgical lists at each participating site | |
| Feeding control participants 15 healthy adult members of NHS staff will be recruited to participate in the feeding control sub-study at University Hospitals Coventry and Warwickshire NHS Trust only. | |
| Spasticity sub-group controls 10 healthy adult members of NHS staff at University Hospitals of North Midlands, will be recruited to take part as spasticity sub-study controls | |
| Traumatic brain injury patients 10 adult patients with an isolated traumatic brain injury at University Hospitals Coventry and Warwickshire NHS Trust and Imperial College Healthcare NHS Trust. |
资格标准
| 有资格学习的年龄 | 18 Years 至 18 Years |
| 有资格学习的性别 | All |
| 取样方式 | Probability Sample |
| 接受健康志愿者 | 是 |
| 标准 | Main study Stroke patients: Inclusion criteria: - Aged 18 years or over - Admitted to hospital with suspected stroke, diagnosed by a suitably qualified doctor or nurse using an appropriate clinical assessment (e.g. FAST, NIHSS, WHO criteria) - Patient has ongoing symptoms of stroke at the time of enrolment in to the study. Exclusion criteria: - Onset of stroke symptoms >4.5 hours prior to admission or time of onset of symptoms is not known - Patient has undergone thrombolysis during current admission (N.B. patients can be enrolled before planned thrombolysis, but not after) - Patient's stroke symptoms have resolved completely prior to enrolment in the study - The patient, or their consultee if the patient is incapacitated, is unwilling to provide written informed consent Healthy controls Inclusion criteria: - Aged 18 years and over - Scheduled for surgery - Available for follow-up Exclusion criteria: - History of, or evidence of current, cerebrovascular disease, including stroke, transient ischaemic attack, subarachnoid haemorrhage and vascular dementia - History of cardiovascular disease, including myocardial infarction, angina or peripheral vascular disease - History of, or current, gout - Currently receiving chemotherapy - History of diabetes - Unwilling or unable to provide written informed consent Feeding Controls sub-study Inclusion criteria - Aged 18 years and over - Member of NHS staff - Willing to eat the study meal provided (sandwich with a meat based filling) Exclusion criteria - Current acute health problems requiring medical review, investigation, or treatment - History of, or evidence of current, cerebrovascular disease, including stroke, transient ischaemic attack, subarachnoid haemorrhage and vascular dementia - History of cardiovascular disease, including myocardial infarction, angina or peripheral vascular disease - History of, or current, gout - Currently receiving chemotherapy - History of diabetes - Unwilling or unable to provide written informed consent Spasticity sub-study Stroke patients Inclusion criteria - Patients with suspected stroke, who have been enrolled on to the main study at UHNM Exclusion criteria - Unwilling or unable to provide written informed consent to the spasticity sub-study Controls Inclusion criteria - Aged 18 years and over - Member of NHS staff - Willing to complete follow-up visits Exclusion criteria - Current acute health problems requiring medical review, investigation, or treatment - History of, or evidence of current, cerebrovascular disease, including stroke, transient ischaemic attack, subarachnoid haemorrhage and vascular dementia - History of cardiovascular disease, including myocardial infarction, angina or peripheral vascular disease - History of gout - Currently receiving chemotherapy - History of diabetes - Unwilling or unable to provide written informed consent Traumatic brain injury sub-study Inclusion criteria: - Aged 18 years or over - Admitted to hospital with Traumatic brain injury, diagnosed by a suitably qualified doctor with CT scan supporting evidence of a bleed and/or clot. Exclusion criteria: - The patient, or their consultee if the patient is incapacitated, is unwilling to provide written informed consent - History of Cancer |
结果
主要结果指标
1. To determine whether blood purine levels in stroke patients at admission correlate with infarct/ haemorrhage size as determined by MRI or CT scan at 24-72 hours after onset of symptoms [24-72h after onset of symptoms]
