Immunological Consequences of CARD15/NOD2 Mutations in Crohn's Disease
关键词
抽象
描述
Crohn's Disease (CD) affects about 60.000 to 80.000 people in France. It is characterised by a chronic or relapsing inflammation of the gut. Its aetiology is largely unknown, limiting the development of preventive and curative therapeutic options. In 2001, we have identified the first CD susceptibility gene: CARD15/NOD2. This gene is involved in the innate immune response but we do not know today how gene mutations may induce the disease lesions. However, many data suggest that the intestinal inflammation may be related with an abnormal function of the lymphoid tissue present in the gut.
The aim of the present proposal is to better understand the role of CARD15/NOD2 mutations (R702W, G908R and 1007fs) in the development and the function of the intestinal lymphoid tissue. 250 patients and controls will be enrolled at Robert Debré and Saint Louis Hospital (Paris, France) within a 2 years period.
For participant, 4 to 5 ileal biopsies will be taken during a routine colonoscopy. Patients will be classed in three groups according to their number of CARD15/NOD2 mutations (1) wild-type, 2) mutated heterozygotes and 3) mutated homozygotes or compound heterozygotes). A comparison between groups and with non inflammatory (colonoscopy performed for other reasons) or inflammatory (ulcerative colitis patients) controls will be done. For each group, cell phenotype, cytokine profile, permeability and bacterial translocation will be analysed on Peyer's patches.
The project is based on adult and paediatric cohorts among the largest ones in Paris and located at Saint Louis and Robert Debré hospitals. Experiments will be performed at INSERM Unit U843 in collaboration with the department of immunology and statistics, Robert Debré Hospital.
日期
最后验证: | 06/30/2012 |
首次提交: | 10/26/2008 |
提交的预估入学人数: | 10/26/2008 |
首次发布: | 10/27/2008 |
上次提交的更新: | 08/01/2012 |
最近更新发布: | 08/02/2012 |
实际学习开始日期: | 12/31/2008 |
预计主要完成日期: | 12/31/2011 |
预计完成日期: | 12/31/2011 |
状况或疾病
干预/治疗
Other: 1: Crohn's disease patient
相
手臂组
臂 | 干预/治疗 |
---|---|
Experimental: 1: Crohn's disease patient intestinal biopsies | Other: 1: Crohn's disease patient intestinal biopsies during routine endoscopy |
资格标准
有资格学习的年龄 | 5 Years 至 5 Years |
有资格学习的性别 | All |
接受健康志愿者 | 是 |
标准 | Inclusion Criteria: - Children and adults ≥ 5 ans and ≤ 65 ans with a programmed routine colonoscopy for Crohn's disease, ulcerative colitis, or another benign gastroenterological disorder Exclusion Criteria: - Patients < 5 ans or > 65 ans - Patients with contra-indications for multiple biopsies - Patients with an undeterminate colitis |
结果
主要结果指标
1. cytokine production and cell phenotype in Peyer patches [final time frame at the end of the study]