Impact of Pumps on Internal Thoracic Arteries (IPITA)
关键词
抽象
描述
Eighty elective male patients undergoing CABG were selected using one or two internal thoracic arteries under CPB with a roller pump (RP group) or centrifugal pump (CFP group). ITA samples were collected before starting CPB (Time 1) and before the last coronary anastomosis during aortic cross clamping (Time 2). Terminal complement complex activation (SC5b-9) and neutrophil activation (elastase) analysis were performed on arterial blood at the same times.
日期
最后验证: | 10/31/2019 |
首次提交: | 11/13/2019 |
提交的预估入学人数: | 11/17/2019 |
首次发布: | 11/18/2019 |
上次提交的更新: | 11/18/2019 |
最近更新发布: | 11/20/2019 |
实际学习开始日期: | 11/02/2015 |
预计主要完成日期: | 11/07/2017 |
预计完成日期: | 12/07/2017 |
状况或疾病
干预/治疗
Diagnostic Test: vascular reactivity of internal thoracic arteries
相
手臂组
臂 | 干预/治疗 |
---|---|
Other: Roller pump group CABG was performed under normothermic (36-37°C) cardiopulmonary bypass (CPB). All components of the circuits were coated with phosphorylcholine inert surface (PHISIO, Sorin®). The pump manufacturer is Maquet® for the roller pumps.1.5 cm of ITA distality was sampled before blood flow interruption into the graft and before starting CPB (Time 1) and another segment (1.5 cm) before the last coronary anastomosis during aortic cross clamping (Time 2) (Figure 1). Each arterial segment was cut into three parts: a fresh part for arterial myography bathed and stored in a 50 ml organ bath containing a physiological salt solution (PSS). The other two parts were cooled in liquid nitrogen and stored at -80°C for immunohistochemistry and RT-PCR analysis. | |
Other: Centrifugal pump group CABG was performed under normothermic (36-37°C) cardiopulmonary bypass (CPB). All components of the circuits were coated with phosphorylcholine inert surface (PHISIO, Sorin®). The pump manufacturer is Sorin® for the centrifugal pumps.1.5 cm of ITA distality was sampled before blood flow interruption into the graft and before starting CPB (Time 1) and another segment (1.5 cm) before the last coronary anastomosis during aortic cross clamping (Time 2) (Figure 1). Each arterial segment was cut into three parts: a fresh part for arterial myography bathed and stored in a 50 ml organ bath containing a physiological salt solution (PSS). The other two parts were cooled in liquid nitrogen and stored at -80°C for immunohistochemistry and RT-PCR analysis. |
资格标准
有资格学习的年龄 | 18 Years 至 18 Years |
有资格学习的性别 | Male |
接受健康志愿者 | 是 |
标准 | Inclusion Criteria: - male patients and elective coronary artery bypass grafting using at least one of the two ITAs. Exclusion Criteria: - female patients because their complement activation has been shown to be greater than that in men during surgery under cardiopulmonary bypass ; age < 18 years; CABG requiring additional valve repair or replacement; emergency surgery and insufficient length of the internal thoracic artery |
结果
主要结果指标
1. Myography [1 day]
次要成果指标
1. Superoxide detection and confocal microscopy [1 day]
2. Immunochemistry [1 day]
3. Quantitative real time transcription-polymerase chain reaction (RT-PCR) analysis [1 day]
4. Blood sampling and biochemical analysis [2 hours]