Infant Immune Response to Bacterial Infection
关键词
抽象
描述
Bacterial infection in preterm neonates is a significant clinical problem. Neutrophils play a critical role in the bactericidal process, which is immature in newborns though their peripheral blood neutrophil counts are similar to or higher than that of older children and adults. The use of steroids to enhance lung maturation in premature infants complicates this problem. Many studies have shown different biological activities between lipopolysaccharide (LPS) activated neonatal cord blood neutrophils and adult peripheral blood neutrophils. In spite of a number of previous studies, the genetic basis of this differential response to LPS-activation remains unknown. In this study, we will identify the differential gene expression profiles of LPS-activated adult peripheral blood versus cord blood neutrophils from both term and preterm infants by whole genome ligonucleotide microarray. We will investigate the priming effect of histologic chorioamnionitis (HCA), a severe infection/inflammation implicated in many neonatal diseases, on the immune response in neutrophils in terms of gene expression. The effect of prenatal administration of steroids on the immune response in preterm neutrophils is another question we will address in the proposed study. This study will provide insight into the molecular basis for genetic regulation of neutrophil development. We expect to identify novel genes with differential expressions in LPS-activated cord blood neutrophils when compared to those of LPS-activated adult cells. Aberrant transcripts due to the priming effect of HCA to the immune response in term neutrophils will also provide insight on the defense mechanism of subsequent exposures to bacterial infection.
This is a collaborative study. All samples will be collected by our Collaborators at the Chinese University of Hong Kong, Hong Kong. We will perform expression profiling in the Laboratory of Clinical Genomics at NICHD. This protocol has been approved by the Joint Clinical Research Ethics Committee of the Chinese University of Hong Kong and Hospital Authority of Hong Kong.
日期
最后验证: | 03/28/2011 |
首次提交: | 10/16/2007 |
提交的预估入学人数: | 10/16/2007 |
首次发布: | 10/17/2007 |
上次提交的更新: | 06/29/2017 |
最近更新发布: | 07/01/2017 |
实际学习开始日期: | 10/14/2007 |
预计完成日期: | 03/28/2011 |
状况或疾病
相
资格标准
有资格学习的性别 | All |
接受健康志愿者 | 是 |
标准 | - INCLUSION CRITERIA: Chinese pregnant women admitted to the labor ward of the Prince of Wales hospital. Normal spontaneous delivery. EXCLUSION CRITERIA: Patient diagnosed with known infectious diseases such as HIV and HBV positive cases (blood precautions). Patients are incompetent to consent such as being mentally retarded or mentally ill and without an impairment of judgment at the time of consenting. Patients who do not consent. |