Lipoprotein Metabolism and Bacterial Lipopolysaccharide in Parkinson's Disease
关键词
抽象
描述
Bacterial lipopolysaccharides are able to produce neuroinflammation and dopaminergic receptors degeneration. In addition, they may produce an accumulation of α-synuclein in the area of the substantia Nigra. Recent studies have shown that α-synuclein aggregates may be present also in gastrointestinal neurons of patients with PD. This last finding led to the hypothesis that the intestine might be an early site of PD disease in response to an environmental toxin or pathogen. Forsyth et al. have discovered an impaired intestinal permeability in subjects with recently diagnosed PD, and they found positive correlations between this factor, exposure to LPS and alpha-synuclein accumulation in gastrointestinal neurons. Plasma lipoproteins play a key role in the detoxification of bacterial endotoxins. Lipoprotein chemical composition is related to their detoxing properties. To the best of investigator knowledge, the relationships between lipoprotein chemical composition and LPS in PD have not yet been investigated. Therefore, the aims of this study are: I) to evaluate the chemical composition of VLDL, LDL and HDL in subjects with PD compared to a control group; 2) to analyze the activity of plasma lipid transfer proteins and LPS plasma levels in the same groups of subjects; III) finally, to investigate the correlations between the analyzed parameters.
Subjects and method Twenty patients with PD and twenty healthy controls were recruited for the study. Fasting blood samples were taken for routine laboratory analysis and for the separation of EDTA plasma. Plasma samples stored at -80°C until were used for lipoprotein isolation and analysis and for the measurement of lipid transfer protein and LPS levels.
日期
| 最后验证: | 04/30/2019 |
| 首次提交: | 04/15/2019 |
| 提交的预估入学人数: | 05/01/2019 |
| 首次发布: | 05/02/2019 |
| 上次提交的更新: | 05/01/2019 |
| 最近更新发布: | 05/02/2019 |
| 实际学习开始日期: | 05/02/2016 |
| 预计主要完成日期: | 10/19/2017 |
| 预计完成日期: | 10/19/2017 |
状况或疾病
干预/治疗
Other: Patients not treated
相
手臂组
| 臂 | 干预/治疗 |
|---|---|
| Parkinson's patients Patients with Parkinson disease evaluated in agreement with UK Brain Bank criteria | |
| Control group Subject matched for sex, age and BMI |
资格标准
| 有资格学习的年龄 | 18 Years 至 18 Years |
| 有资格学习的性别 | All |
| 取样方式 | Non-Probability Sample |
| 接受健康志愿者 | 是 |
| 标准 | Parkinson's patients Inclusion Criteria: - Diagnosis of Parkinson disease in agreement with UK Brain Bank - Farmacological treatment with L-Dopa and/or dopaminergic agonist or diagnosis de novo - BMI 18.5 - 29.9 kg/m^2 - Informed consent signature Exclusion criteria: - Presence of type 1 and type 2 diabetes mellitus - Presence of major chronic diseases of the digestive tract. - Pregnancy in progress - Subjects subjected to antihypertensive therapies or statins or with drugs that can change metabolic status and insulin sensitivity (e.g. chronic oral steroid therapy) - Subjects affected by endocrine pathologies (e.g. Cushing disease, uncontrolled thyroid disease) - Presence of known renal insufficiency or creatinine levels greater than 1.8 mg/dl - Presence of chronic liver disease or ALT and AST levels exceeding two standard deviations from normal levels - Presence of malignant disease - Alcohol or drug abuse - Major psychiatric disorders - Subjects dedicated to intense and agonistic physical activity. Control group Inclusion criteria - Absence of major disease - BMI 18.5 - 29.9 kg/m^2 - Informed consent signature Exclusion Criteria: - Presence of type 1 and type 2 diabetes mellitus - Presence of major chronic diseases of the digestive tract. - Pregnancy in progress - Subjects subjected to antihypertensive therapies or statins or with drugs that can change metabolic status and insulin sensitivity (e.g. chronic oral steroid therapy) - Subjects affected by endocrine pathologies (e.g. Cushing disease, uncontrolled thyroid disease) - Presence of known renal insufficiency or creatinine levels greater than 1.8 mg/dl - Presence of chronic liver disease or ALT and AST levels exceeding two standard deviations from normal levels - Presence of malignant disease - Alcohol or drug abuse - Major psychiatric disorders - Subjects dedicated to intense and agonistic physical activity. |
结果
主要结果指标
1. LPS [through study completion an average of 1 year]
2. Lipoprotein chemical composition [through study completion an average of 1 year]
次要成果指标
1. Plasma lipid transfer proteins [through study completion an average of 1 year]
