Sildenafil Activates Browning of White Adipose and Improves Insulin Sensitivity
关键词
抽象
描述
The study subjects will be taken placebo (first stage) and sildenafil (second stage) intervention for 7 days, respectively afterward.Subcutaneous fat tissue and muscle samples will be obtained by biopsy from some individuals and measure the browning of white adipose tissue and insulin signaling.The insulin sensitivity will be tested by insulin clamp assay before and after each intervention, respectively. In addition,blood samples for biochemical analysis will be obtained before and after each intervention, respectively.
The browning of white adipose tissue will be measured by the expressions of peroxisome proliferator-activated receptor-γ (PPARγ), PPARγcoactivator 1α (PGC-1α), uncoupling protein 1 (UCP-1), the second messenger cyclic guanosine-3', 5'-monophosphate (cGMP),PR domain containing 16 zinc finger transcription factor (Prdm16) and deiodinase, iodothyronine, type II (DIO2).The metabolic makers will be measured by blood pressure, heart rate, thyroid functions, resting metabolic rate, respiratory quotient, blood cGMP, blood insulin and blood glucose.
日期
最后验证: | 07/31/2016 |
首次提交: | 08/01/2015 |
提交的预估入学人数: | 08/10/2015 |
首次发布: | 08/13/2015 |
上次提交的更新: | 08/30/2016 |
最近更新发布: | 09/01/2016 |
实际学习开始日期: | 07/31/2015 |
预计主要完成日期: | 08/31/2016 |
预计完成日期: | 08/31/2016 |
状况或疾病
干预/治疗
Drug: Sildenafil
Drug: Placebo group
相
手臂组
臂 | 干预/治疗 |
---|---|
Experimental: Sildenafil Eleven patients receive sildenafil 100mg/day (25 mg at 8 AM plus 25 mg at 4 PM plus 50 mg at 10 PM)for 7 days. | Drug: Sildenafil sildenafil 100 mg per day for 7 days. |
Placebo Comparator: Placebo group An identical placebo for 7 days in placebo group. | Drug: Placebo group an identical placebo per day for 7 days |
资格标准
有资格学习的年龄 | 20 Years 至 20 Years |
有资格学习的性别 | Male |
接受健康志愿者 | 是 |
标准 | Inclusion Criteria: - overweight volunteers - 20-30 years old - body mass index >=25 kg/m2 - normal glucose tolerance Exclusion Criteria: - normal body mass index - abnormal cardiovascular status - women - history of any local or systemic infectious disease with fever or requiring antibiotic within four weeks of drug administration - current addiction to alcohol or substances of abuse - children - current addiction to alcohol or substances of abuse - mental incapacity - the use of any medication within four weeks - subjects with hyperthyroidism or hypothyroidism, hypertension (even if controlled with medications) |
结果
主要结果指标
1. Browning of white adipose tissue [7 days]
次要成果指标
1. Improvement of insulin sensitivity [7days]
其他成果措施
1. Change of metabolic status [3 months]