Vitamin A Absorption From Cassava in Women
关键词
抽象
描述
We plan to conduct a single site intervention phase II intervention study in healthy adult women. Women will serve as their own controls, and will be fed three treatments in random order: a single meal of low-carotenoid cassava porridge with fat, a similar meal of bio-fortified cassava (that is high in vitamin A-forming carotenoids) with fat, and a similar meal of bio-fortified cassava without fat. Carotenoids and vitamin A will be measured in the triacylglycerol-rich lipoprotein (TRL) fraction of plasma by standard methods involving ultracentrifugation (to concentrate the TRL fraction) followed by reversed-phase HPLC using electrochemical detection. We plan to collect sufficient data to identify the times of the first appearance and peak concentrations in retinol, retinyl esters, alpha-carotene (AC), beta-carotene (BC), beta-carotene isomers (BCI), and cryptoxanthin (CX) in TRL: when a single meal containing moderately high amounts of carotenoids from bio-fortified cassava is fed to healthy adult women. We will use this data to determine the vitamin A equivalency of bio-fortified cassava in adult women, and the effect of fat on vitamin A equivalency of bio-fortified cassava.
日期
| 最后验证: | 11/30/2011 |
| 首次提交: | 06/21/2011 |
| 提交的预估入学人数: | 06/22/2011 |
| 首次发布: | 06/26/2011 |
| 上次提交的更新: | 12/07/2011 |
| 最近更新发布: | 12/11/2011 |
| 实际学习开始日期: | 05/31/2011 |
| 预计主要完成日期: | 09/30/2011 |
| 预计完成日期: | 09/30/2011 |
状况或疾病
干预/治疗
Other: Cassava Treatment 1
Other: Cassava Treatment 2
Other: Cassava Treatment 3
相
手臂组
| 臂 | 干预/治疗 |
|---|---|
| Experimental: Cassava Treatment 1 Single meal containing bio-fortified, high carotenoid cassava without oil. | Other: Cassava Treatment 1 300 g of porridge (11 ounces) containing 100 g drained mashed cassava and approximately 1 mg beta-carotene. |
| Experimental: Cassava Treatment 2 Single meal containing bio-fortified, high carotenoid cassava with oil. | Other: Cassava Treatment 2 300g porridge (11 ounces) containing 100 g drained mashed cassava, 15 g peanut oil, and approximately 1 mg beta-carotene. |
| Active Comparator: Cassava Treatment 3 Single meal containing low carotenoid cassava with oil and retinyl palmitate. | Other: Cassava Treatment 3 300 g porridge containing 15 mL peanut oil, 100 g drained mashed cassava, and a tracer of approximately .3 mg pure food-grade retinyl palmitate. |
资格标准
| 有资格学习的年龄 | 18 Years 至 18 Years |
| 有资格学习的性别 | Female |
| 接受健康志愿者 | 是 |
| 标准 | Inclusion Criteria: - non-smoking - BMI between 18-30 - total cholesterol concentrations between 90 and 225 mg/dL - blood pressure under 140/90 mm Hg - hemoglobin above 11.5 g/dL - blood chemistries within the normal range Exclusion Criteria: - must not be pregnant or trying to get pregnant - must not be taking fat, triacylglycerol, or cholesterol lowering medications) such as orlistat, gemfibrozil, niacin, lovastatin, simvastatin, colestipol, and ezetimibe) - must not taking medicines containing high doses of retinoids such as Accutane or high vitamin A or carotenoid supplements - must not have blood chemistry or health history results consistent with acute cancer or heart disease - no obvious psychological or sociological problems such as alcoholism, drug abuse, or severe and acute mental illness that would influence their ability to sign an informed consent agreement or to participate in study duties and activities - must not be allergic to cassava, peanuts, or peanut oil |
结果
主要结果指标
1. Vitamin A equivalency from varieties of cassava with and without oil [0, 60, 150, 240, 330, 420, 510, 600, 1440 min]
