Biological control of crown gall disease on grapevines

BACKGROUND OF THE INVENTION

This invention relates to biological control of crown gall disease on grapevines as well as enhancing grapevine development.

Crown gall disease of grapevine caused by Agrobacterium vitis is a limiting factor to grape production in several regions of the world (Burr et al. (1999) Annu. Rev. Phytopathol. 37:53-80). A. vitis survives systemically in grapevines and therefore is disseminated in propagation material (Burr et al. (1984) Plant Dis. 68:976-978; Burr and Katz (1983) Phytopathology 73:163-165; Goodman et al. (1987) Am. J. Enol. Vitic 38:189-193). Like crown gall on other plant species, tumorigenesis results following the transfer of a set of oncogenes called the T-DNA that are carried on a bacterial Ti plasmid; the T-DNA becomes integrated and expressed in the plant host (Chilton et al., (1977) Cell 11: 263-271) leading to crown gall. A. vitis strains can be grouped based on the type of Ti plasmid they carry and may also be designated by the opine catabolic gene(s) that resides on their Ti plasmid (Otten et al. (1996) Mol. Plant-Microbe Interact. 9:782-786).

There are no effective chemical controls to combat crown gall and therefore the interest in developing a biological control. Biological control of crown gall on specific crops, such as stone fruits has been successfully implemented in commercial agriculture using A. radiobacter strain K84 (Farrand, (1990) In R. R. Baker, and P. E. Dunn (Eds.) pp. 679-691). K84 is not effective against A. vitis strains on grapevine and therefore several research groups have investigated other candidate biological control strains for crown gall control on grape. Some of the more recent research on biological control of grape crown gall included nontumorigenic A. vitis strain E26 (Liang et al. (1990) Acta Microbio Sin 30:165-171) and strain VAR03-1 (Kawaguchi et al. (2008) Phytopathology 98:1218-1225). Other strains including Rahnella aquatilis strain HX2 (Chen et al. (2007) Plant Disease 91: 957-963), Serratia plymuthica strain IC1270, Pseudomonas fluorescens strain Q8r1-96, P. fluorescens strain B-4117 (Dandurishvili et al. (2010) Journal of Applied Microbiology 110: 341-352) and others were shown to provide various levels of grape crown gall inhibition under experimental conditions (Eastwell et al. (2006) Crop Protection 25:1191-1200).

Accordingly, there is a need in the art for effective controls of crown gall disease on grapevines. The present invention provides such a need.

SUMMARY OF THE INVENTION

The invention described herein relates to genetically engineered Agrobacterium strains (for example, Agrobacterium vitis F2/5) useful as a biological control agent for the prevention of crown gall disease caused by the infection of nursery stock and vineyards by many virulent strains of Agrobacterium such as A. vitis.

Accordingly, in general, the invention features genetic derivatives of a non-tumorigenic Agrobacterium vitis strain F2/5. In particular, such derivatives have a reduced ability to cause a necrosis of grape tissue while retaining biological control of crown gall disease. Identification of such derivatives is accomplished through standard methods such as those described herein.

In one aspect, the invention features a necrosis-minus, Agrobacterium vitis derivative of strain F2/5, wherein the derivative retains crown gall biological control. Exemplary derivatives are typically provided in an isolated or pure form. In one embodiment, the necrosis-minus A. vitis derivative of F2/5 has an inactivated gene encoding phosphopantetheinyl transferase (PPTase) (e.g., an inactivated homolog of A. vitis S4 (avi5813)), an inactivated gene encoding aminotransferase (e.g., an inactivated homolog of A. vitis S4 (avi4329)), an inactivated gene encoding iron response regulator (e.g., an inactivated rirA homolog of A. vitis S4 (avi0838)); or a combination thereof. Other genes of necrosis which may be inactivated to engineer a necrosis-minus A. vitis derivative of strain F2/5 include a member of transcriptional regulator LuxR family aviR (F-avi4374), a proteolytic subunit of ATP-dependent Clp protease clpP1(F-avi1696), nonribosomal peptide synthetase (F-avi3342) and polyketide synthase (F-avi4330). Methods for inactivating such genes in bacteria are well known in the art and include, without limitation, standard methods such as gene disruption by insertion of a DNA fragment and gene replacement in which the target gene is replaced by a select marker such as antibiotic resistance gene via double recombination at flanking regions of the target gene as well as the methods and biological control assays for analyzing such derivatives as is disclosed herein.

Exemplary sequences for A. vitis strain F2/5 related to necrosis and crown gall biological control are listed in Table 1.

TABLE-US-00001 TABLE 1 F-avi5813 Nucleic acid (735 nucleotides) SEQ ID NO: 1 F-avi5813 Protein (244 amino acids) SEQ ID NO: 2 F-avi4329 Nucleic acid (1368 nucleotides) SEQ ID NO: 3 F-avi4329 Protein (455 amino acids) SEQ ID NO: 4 F-avi0838 Nucleic acid (471 nucleotides) SEQ ID NO: 5 F-avi0838 Protein (156 amino acids) SEQ ID NO: 6 F-avi4374 Nucleic acid (741 nucleotides) SEQ ID NO: 7 F-avi4374 Protein (246 amino acids) SEQ ID NO: 8 F-avi1696 Nucleic acid (633 nucleotides) SEQ ID NO: 9 F-avi1696 Protein (210 amino acids) SEQ ID NO: 10 F-avi3342 Nucleic acid (4005 nucleotides) SEQ ID NO: 11 F-avi3342 Protein (1334 amino acids) SEQ ID NO: 12 F-avi4330 Nucleic acid (7524 nucleotides) SEQ ID NO: 13 F-avi4330 Protein (2187 amino acids) SEQ ID NO: 14 Avs locus Nucleic acid (11899 nucleotides) SEQ ID NO: 15 P1388 Nucleic acid (471 nucleotides) SEQ ID NO: 16 A. vitis strain Protein (156 amino acids) - SEQ ID NO: 17 F2/5 transposase P1389 Nucleic acid (1173 nucleotides) SEQ ID NO: 18 A. vitis strain Protein (390 amino acids) - SEQ ID NO: 19 F2/5 hypothetical P1390 Nucleic acid (1206 nucleotides) SEQ ID NO: 20 A. vitis strain Protein (401 amino acids) - multidrug SEQ ID NO: 21 F2/5 resistant protein P1391 Nucleic acid (1893 nucleotides) SEQ ID NO: 22 A. vitis strain Protein (630 amino acids) - SEQ ID NO: 23 F2/5 siderophore synthetase P1392 Nucleic acid (1137 nucleotides) SEQ ID NO: 24 A. vitis strain Protein (378 amino acids) - tauropine SEQ ID NO: 25 F2/5 dehydrogenase P1393 Nucleic acid (1011 nucleotides) SEQ ID NO: 26 A. vitis strain Protein (336 amino acids) - cysteine SEQ ID NO: 27 F2/5 synthetase P1394 Nucleic acid (1398 nucleotides) SEQ ID NO: 28 A. vitis strain Protein (465) - diaminopimelate SEQ ID NO: 29 F2/5 decarboxylase P1395 Nucleic acid (501 nucleotides) SEQ ID NO: 30 A. vitis strain Protein (166 amino acids) SEQ ID NO: 31 F2/5 P1396 Nucleic acid (2154 nucleotides) SEQ ID NO: 32 A. vitis strain Protein (716 amino acids) - SEQ ID NO: 33 F2/5 ferrichrome iron receptor P1397 Nucleic acid (717 nucleotides) SEQ ID NO: 34 A. vitis strain Protein (238 amino acids) - SEQ ID NO: 35 F2/5 transposase F-avi5730 Nucleic acid (3879 nucleotides) SEQ ID NO: 36 F-avi5730 Protein (1292 amino acids) SEQ ID NO: 37

In another aspect, the invention features a biologically-pure culture including a necrosis-minus, A. vitis derivative of strain F2/5, wherein the derivative retains crown gall biological control. Exemplary derivatives are described herein.

In another aspect, the invention features a composition including a necrosis-minus, A. vitis derivative of strain F2/5, wherein the derivative retains crown gall biological control. Exemplary derivatives are described herein.

In still other aspects, the invention features a grapevine or grapevine component including a necrosis-minus, A. vitis derivative of strain F2/5, wherein the derivative retains crown gall biological control. Exemplary derivatives are described herein. In one embodiment, the grapevine component is a somatic embryo, a seed, a seedling, a scion, a rootstock, a cane, a cutting (e.g., a green cutting or a dormant cutting), a leaf, a stem, or a root. In another embodiment, the grapevine or grapevine component includes one or more of the F2/5 derivatives, described herein.

Exemplary grape plants for use in the practice of the invention include, but are not limited to, grapevines (for example, Vitis spp., Vitis spp. hybrids, and all members of the subgenera Euvitis and Muscadinia), including scion or rootstock cultivars. Exemplary scion cultivars include, without limitation, those which are referred to as table or raisin grapes and those used in wine production such as Cabernet Franc, Cabernet Sauvignon, Chardonnay (for example, CH 01, CH 02, CH Dijon), Merlot, Pinot Noir (PN, PN Dijon), Semillon, White Riesling, Lambrusco, Thompson Seedless, Autumn Seedless, Niagrara Seedless, and Seval Blanc. Other scion cultivars which can be used include those commonly referred to as Table or Raisin Grapes, such as Alden, Almeria, Anab-E-Shahi, Autumn Black, Beauty Seedless, Black Corinth, Black Damascus, Black Malvoisie, Black Prince, Blackrose, Bronx Seedless, Burgrave, Calmeria, Campbell Early, Canner, Cardinal, Catawba, Christmas, Concord, Dattier, Delight, Diamond, Dizmar, Duchess, Early Muscat, Emerald Seedless, Emperor, Exotic, Ferdinand de Lesseps, Fiesta, Flame seedless, Flame Tokay, Gasconade, Gold, Himrod, Hunisa, Hussiene, Isabella, Italia, July Muscat, Khandahar, Katta Kourgane, Kishmishi, Loose Perlette, Malaga, Monukka, Muscat of Alexandria, Muscat Flame, Muscat Hamburg, New York Muscat, Niabell, Niagara, Olivette blanche, Ontario, Pierce, Queen, Red Malaga, Ribier, Rish Baba, Romulus, Ruby Seedless, Schuyler, Seneca, Suavis (IP 365), Thompson seedless, and Thomuscat. They also include those used in wine production, such as Aleatico, Alicante Bouschet, Aligote, Alvarelhao, Aramon, Baco blanc (22A), Burger, Cabernet franc, Caberet, Sauvignon, Calzin, Carignan, Charbono, Chardonnay, Chasselas dore, Chenin blanc, Clairette blanche, Early Burgundy, Emerald Riesling, Feher Szagos, Fernao Pires, Flora, French Colombard, Fresia, Furmint, Gamay, Gewurztraminer, Grand noir, Gray Riesling, Green Hungarian, Green Veltliner, Grenache, Grillo, Helena, Inzolia, Lagrein, Lambrusco de Salamino, Malbec, Malvasia bianca, Mataro, Melon, Merlot, Meunier, Mission, Montua de Pilas, Muscadelle du Bordelais, Muscat blanc, Muscat Ottonel, Muscat Saint-Vallier, Nebbiolo, Nebbiolo fino, Nebbiolo Lampia, Orange Muscat, Palomino, Pedro Ximenes, Petit Bouschet, Petite Sirah, Peverella, Pinot noir, Pinot Saint-George, Primitivo di Gioa, Red Veltliner, Refosco, Rkatsiteli, Royalty, Rubired, Ruby Cabernet, Saint-Emilion, Saint Macaire, Salvador, Sangiovese, Sauvignon blanc, Sauvignon gris, Sauvignon vert, Scarlet, Seibel 5279, Seibel 9110, Seibel 13053, Semillon, Servant, Shiraz, Souzao, Sultana Crimson, Sylvaner, Tarmat, Teroldico, Tinta Madeira, Tinto cao, Touriga, Traminer, Trebbiano Toscano, Trousseau, Valdepenas, Viognier, Walschriesling, White Riesling, and Zinfandel.

Rootstock cultivars that are useful in the invention include, without limitation, Vitis rupestris Constantia, Vitis rupestris St. George, Vitis california, Vitis girdiana, Vitis rotundifolia, Vitis rotundifolia Carlos, Richter 110 (Vitis berlandieri.times.rupestris), 101-14 Millarder et de Grasset (Vitis riparia.times.rupestris), Teleki 5C (Vitis berlandieri.times.riparia), 3309 Courderc (Vitis riparia.times.rupestris), Riparia Gloire de Montpellier (Vitis riparia), 5BB Teleki (selection Kober, Vitis berlandieri.times.riparia), SO.sub.4 (Vitis berlandieri.times.rupestris), 41B Millardet (Vitis vinifera.times.berlandieri), and 039-16 (Vitis vinifera.times.Muscadinia). Additional rootstock cultivars which can be used include Couderc 1202, Couderc 1613, Couderc 1616, Couderc 3309, Dog Ridge, Foex 33EM, Freedom, Ganzin 1 (A.times.R #1), Harmony, Kober 5BB, LN33, Millardet & de Grasset 41B, Millardet & de Grasset 420A, Millardet & de Grasset 101-14, Oppenheim 4 (SO.sub.4), Paulsen 775, Paulsen 1045, Paulsen 1103, Richter 99, Richter 110, Riparia Gloire, Ruggeri 225, Saint-George, Salt Creek, Teleki 5A, Vitis rupestris Constantia, Vitis california, and Vitis girdiana.

In still another aspect, the invention features a method for reducing crown gall disease on a grapevine or grapevine component, the method including administering to a grapevine or grapevine component an effective amount of a necrosis-minus, A. vitis derivative of strain F2/5, wherein the derivative retains crown gall biological control; a culture including a necrosis-minus, A. vitis derivative of strain F2/5, wherein the derivative retains crown gall biological control or a composition including a necrosis-minus, A. vitis derivative of strain F2/5, wherein the derivative retains crown gall biological control. Exemplary derivatives are described herein. In one embodiment, one or more of the bacterial derivatives, culture, or composition is administered at grafting time. In another embodiment, one or more of the bacterial derivatives, culture, or composition is administered on graft unions or the base of grapevine. In still another embodiment, one or more of the bacterial derivatives, culture, or composition is administered during field grafting of grapevine. In this case, the biocontrol agent is applied when vinyardists are top-grafting vines; a process that is used to change varieties in vineyards without removing current vines. In other embodiments, one or more of the bacterial derivatives, culture, or composition is administered to a dormant grapevine cutting. The biocontrol agent may also be applied to the basal end of each grafted or nongrafted vine to control crown gall and to stimulate callus and root formation. In certain embodiments, one or more of the bacterial derivatives, culture, or composition is administered to a dormant cane cutting or to a green shoot cutting. And in yet other embodiments, one or more of the bacterial derivatives, culture, or composition is administered to grapevine plant part above the ground (e.g., a trunk or cane). If desired, one or more of the necrosis-minus F2/5 derivatives is administered in any of the aforementioned embodiments.

In still another aspect, the invention features a method for reducing necrosis on a grapevine or grapevine component, the method including administering to a grapevine or grapevine component an effective amount of one or more of bacterial derivatives, cultures, or compositions described herein, wherein the method reduces necrosis on the grapevine or grapevine component. Administration of the derivative of F2/5, culture, or composition is administered at grafting time; for example, on graft unions or the base of grapevine. In other embodiments, administration occurs during field grafting of grapevine or to a dormant cane cutting prior to rooting. In other embodiments administration is to a green shoot cutting. Typically, the bacterial derivatives, culture, or composition, in this method, is administered to grapevine plant part which grows below the ground. If desired, one or more of the necrosis-minus F2/5 derivatives is administered in any of the aforementioned embodiments.

In still another aspect, the invention features a method for controlling crown gall disease, the method including administering to a locus for planting a grapevine or grapevine component an effective amount of the derivatives of F2/5, cultures, or compositions described herein, wherein the method controls crown gall disease. In one embodiment, the locus is a furrow or soil (e.g., the area where grape plants of a vineyard are to be planted). If desired, one or more of the necrosis-minus F2/5 derivatives is administered in any of the aforementioned embodiments.

In still another embodiment, the invention features a method for identifying an A. vitis F2/5 as a biocontrol agent for crown gall disease, the method including the steps of: a) administering the derivative of F2/5, culture, or composition to a grapevine or grapevine component; and b) assaying incidence of crown gall disease on the grapevine or grapevine component, wherein a reduction in crown gall disease compared to a control grapevine or grapevine component identifies the A. vitis strain F2/5 as a biocontrol agent for crown gall disease. In one embodiment, the A. vitis strain F2/5 is a biocontrol agent for crown gall disease on a grape plant part growing above the ground (e.g., where the grape plant part is a graft, trunk, or cane). Again, if desired, one or more of the necrosis-minus F2/5 derivatives is administered in any of the aforementioned embodiments.

In another aspect, the invention features a method for identifying an A. vitis F2/5 as a biocontrol agent for necrosis, the method including the steps of: a) administering any one of a derivative of F2/5, culture, or composition to a grapevine or grapevine component; and b) assaying incidence of necrosis on the grapevine or grapevine component, wherein a reduction in necrosis compared to a control grapevine or grapevine component identifies the A. vitis strain F2/5 as a biocontrol agent for necrosis. In one embodiment, the A. vitis strain F2/5 is a biocontrol agent for necrosis on a grape plant part growing below the ground (e.g., where the grape plant part is a root or callus). And still again, if desired, one or more of the necrosis-minus F2/5 derivatives is administered in any of the aforementioned embodiments.

In another aspect, the invention features a method for identifying an A. vitis F2/5 to promote callus development, the method including the steps of: a) administering any one of a derivative of F2/5, culture, or composition to a grapevine or grapevine component; to a grapevine or grapevine component; and b) assaying callus development on the grapevine or grapevine component, wherein an increase in callus development compared to a control grapevine or grapevine component identifies the A. vitis strain F2/5 promoting callus development.

In still another embodiment, the invention features a method for identifying an A. vitis F2/5 to promote root development, the method including the steps of: a) administering any one of a derivative of F2/5, culture, or composition to a grapevine or grapevine component; and b) assaying root development on the grapevine or grapevine component, wherein an increase in development compared to a control grapevine or grapevine component identifies the A. vitis strain F2/5 promoting root development.

In another aspect, the invention features a method of producing an A. vitis resistant vineyard, the method including the steps of: a) administering at least one necrosis-minus necrosis, A. vitis strain F2/5, wherein the A. vitis F2/5 retains crown gall biological control to a grapevine or grapevine component; b) planting the grapevine or grapevine component; c) growing the grapevine or grapevine component to establish the A. vitis resistant vineyard. In certain embodiments, administration of the derivative of F2/5 occurs in the vineyard. The invention accordingly features a vineyard produced according to this method. The invention further features a product derived from a grapevine produced in the vineyard. Such products include grapes, raisins, or other foodstuffs or beverages such as wine or juice.

In another aspect, the invention features a method of engineering a necrosis-minus derivative of A. vitis F2/5, the method including the steps of: a) introducing into an A. vitis F2/5 bacterium a construct which inactivates a gene which induces grape necrosis; and b) determining whether the A. vitis F2/5 induces grape necrosis, wherein a reduction in grape necrosis compared to a control A. vitis F2/5 is taken as identifying a necrosis-minus derivative of A. vitis F2/5. In one embodiment, the gene encodes a PPTase, an aminotransferase or is a homolog of iron response regulator, rirA. Exemplary genes are described herein including homologs of A. vitis S4 avi5813, avi4329, avi0838, avi4374, avi3342, avi1696, or avi3342. In certain embodiments, the gene includes a deletion of all or part of the gene with a suicide vector.

In another embodiment, the invention features a method for promoting callus development, the method including administering to a grapevine or grapevine component an effective amount of any one of a derivative of F2/5, culture, or composition, wherein the method promotes callus development on the grapevine or grapevine component.

In yet another aspect, the invention features a method for promoting root development, the method including administering to a grapevine or grapevine component an effective amount of any one of a derivative of F2/5, culture, or composition, wherein the method promotes root development on the grapevine or grapevine component.

In still another aspect, the invention features a method for reducing necrosis, the method includes administering to a grapevine or grapevine component an effective amount of any one of a derivative of F2/5, culture, or composition, wherein the method reduces necrosis of grapevine or grapevine component tissue growing below ground.

In still other aspects, the invention features an A. vitis bacterium attenuated by a non-reverting mutation in one or more of the PPTase gene (avi5813), the aminotransferase gene (avi4329), and the iron-responsive regulator gene, rirA (avi0838) or a functional homolog of the PPTase gene, aminotransferase gene, or the iron-responsive regulator gene. Such a bacterium is a non-tumorigenic A. vitis, for example, the F2/5 strain. In some embodiments, the invention includes a method for reducing crown gall disease on a grapevine or grapevine component, the method including administering to the grapevine or grapevine component an effective amount of these described A. vitis strains.

In another aspect, the invention features an engineered Agrobacterium bacterium including (a) one or more recombinant genes encoding (i) a sequence including SEQ ID NO: 19 or a functional homolog, (ii) a multidrug resistance protein B (SEQ ID NO: 21) or a functional homolog, (iii) a siderophore synthetase (SEQ ID NO: 23) or a functional homolog, (iv) a tauropine dehydrogenase (SEQ ID NO: 25) or a functional homolog, (v) a cysteine synthetase (SEQ ID NO: 27) or a functional homolog, (vi) diaminopimelate decarboxylase (SEQ ID NO: 29) or a functional homolog, (vii) a sequence including SEQ ID NO: 31 or a functional homolog, or (viii) a ferrichrome-iron receptor (SEQ ID NO: 33) or a functional homolog; and optionally (b) one or more recombinant genes encoding a transposase or a functional homolog. In some embodiments of the engineered Agrobacterium bacterium, the one or more recombinant genes encoding one or more recombinant proteins encode at least one protein listed in FIG. 5A or a functional homolog of at least one protein listed in FIG. 5A. In some embodiments of the engineered Agrobacterium bacterium, expression of an operon including at least a siderophore synthetase or a ferrichrome-iron receptor is controlled by a recombinant promoter, and wherein the promoter is constitutive or inducible. In some other embodiments of the engineered Agrobacterium bacterium, the Agrobacterium is a non-tumorgenic A. vitis, for example, A. vitis F2/5. Typically, the engineered Agrobacterium bacterium is necrosis minus Such engineered bacteria are useful for controlling crown gall disease; in particular, biological control.

In other aspects, the invention features an engineered Agrobacterium bacterium including one or more recombinant gene encoding a sequence including SEQ ID NO: 37. In some embodiments, the engineered Agrobacterium bacterium is a non-tumorgenic A. vitis.

In other aspect, the invention features a method for producing an Agrobacterium species having biological control on grapevine, the method including the steps of (a) providing a non-tumorgenic Agrobacterium species having a control of crown gall disease negative phenotype; and (b) introducing into the Agrobacterium species a sequence including one or more recombinant genes encoding (i) a sequence including SEQ ID NO: 19 or a functional homolog, (ii) a multidrug resistance protein B (SEQ ID NO: 21) or a functional homolog, (iii) a siderophore synthetase (SEQ ID NO: 23) or a functional homolog, (iv) a tauropine dehydrogenase (SEQ ID NO: 25) or a functional homolog, (v) a cysteine synthetase (SEQ ID NO: 27) or a functional homolog, (vi) diaminopimelate decarboxylase (SEQ ID NO: 29) or a functional homolog, (vii) a sequence including SEQ ID NO: 31 or a functional homolog, or (viii) a ferrichrome-iron receptor (SEQ ID NO: 33) or a functional homolog; and optionally, (viiii) a sequence including SEQ ID NO: 37 or a functional homolog thereof.

In other embodiments, the invention features an isolated and purified nucleic acid molecule, obtainable from A. vitis, which consists of at least one gene capable of conferring biological control of crown gall phenotype upon a genetically engineered microorganism, wherein the nucleic acid molecule is contained in the within a locus referred to herein as Avs (SEQ ID NO: 15). In some embodiments, the invention features a plasmid containing such a nucleic acid molecule. In other embodiments, the invention includes a genetically engineered Agrobacterium bacterium having stably and functionally incorporated therein the nucleic acid molecule. Typically, the Agrobacterium bacterium is A. vitis F2/5 such as a derivative that is necrosis minus.

In another aspect, the invention features an isolated nucleic acid molecule, wherein the isolated nucleic acid molecule includes a nucleotide sequence substantially identical to any one of SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36 as described in Table 1 and herein. Typically, nucleic acid molecule includes a heterologous nucleotide sequence that inactivates, diminishes, or abrogates expression of the isolated nucleic acid molecule.

In another aspect, the invention features an isolated nucleic acid molecule, wherein the isolated nucleic acid molecule includes a nucleotide sequence that encodes an amino acid sequence substantially identical to any one of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 as described in Table 1 and herein.

In another aspect, the invention features an engineered Agrobacterium bacterium, wherein the bacterium includes any one of the isolated nucleic acid molecules having a sequence according to SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36 as described in Table 1 and herein.

By "substantially identical" is meant an amino acid sequence or nucleic acid sequence that exhibits at least 50% identity to a reference sequence. Such a sequence is generally at least, e.g., 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identical at the amino acid level or nucleic acid level to a reference sequence. In general, for polypeptides, the length of comparison sequences can be at least five amino acids, e.g., 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 250, 300, or more amino acids, up to the entire length of the polypeptide. For nucleic acids, the length of comparison sequences can generally be at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 250, 300, 400, 500, 600, 700, 800, 900, or more nucleotides, up to the entire length of the nucleic acid molecule. It is understood that for the purposes of determining sequence identity when comparing a DNA sequence to an RNA sequence, a thymine nucleotide is equivalent to a uracil nucleotide.

As used herein, when a polypeptide or nucleic acid sequence is referred to as having "at least X % sequence identity" to a reference sequence, it is meant that at least X percent of the amino acids or nucleotides in the polypeptide or nucleic acid are identical to those of the reference sequence when the sequences are optimally aligned. An optimal alignment of sequences can be determined in various ways that are within the skill in the art, for instance, the Smith Waterman alignment algorithm (Smith et al., J. Mol. Biol. 147:195-7, 1981) and BLAST (Basic Local Alignment Search Tool; Altschul et al., J. Mol. Biol. 215: 403-10, 1990). These and other alignment algorithms are accessible using publicly available computer software such as "Best Fit" (Smith and Waterman, Advances in Applied Mathematics, 482-489, 1981) as incorporated into GeneMatcher Plus.TM. (Schwarz and Dayhof, Atlas of Protein Sequence and Structure, Dayhoff, M. O., Ed pp 353-358, 1979), BLAST, BLAST-2, BLAST-P, BLAST-N, BLAST-X, WU-BLAST-2, ALIGN, ALIGN-2, CLUSTAL, or Megalign (DNASTAR). In addition, those skilled in the art can determine appropriate parameters for measuring alignment, including any algorithms needed to achieve optimal alignment over the length of the sequences being compared.

The invention provides significant advantages for viticulturists, vineyardists, and grape nurserymen among others. Crown gall causes significant economic loss in nurseries worldwide. Application of derivatives of F2/5 offers great promise for controlling this disease and for improving survival and quality of grapevines in the nursery as well as in vineyards. The invention is especially advantageous because the disclosed necrosis-minus necrosis, A. vitis strain F2/5, which retains crown gall biological control minimizes necrosis. Furthermore, this biocontrol agent is not detrimental to grafting, callus formation and rooting of grapevines. Indeed, such bacteria enhance callus and root formation on grapes.

Other features and advantages of the invention will be apparent from the following Drawings, Detailed Description, and Claims.

BRIEF DESCRIPTION OF DRAWINGS

FIG. 1 shows crown gall at wounds on woody grape tissues inoculated with CG49 or CG49 mixed with F2/5 or with F2/5 mutants. Arrows point to inoculated wound sites.

FIG. 2 shows dormant grape cuttings were soaked in suspensions of F2/5, F2/5 mutants or water and then rooted. Arrows point to necrosis on roots.

FIG. 3 shows effects of F2/5 and three necrosis-minus derivatives of F2/5 on grape shoot necrosis.

FIG. 4 shows effects of treatments with A. vitis F2/5 and its mutants on grapevine grafts

FIGS. 5A and 5B show the gene organization and annotation of Avs locus (A) and the effects of knock-down mutant .DELTA.P1391 and .DELTA.P1396 on biological control (B).

FIGS. 6A through 6P show the sequences listed in Table 1.

DETAILED DESCRIPTION

Crown gall of grapevines is caused by the bacterium, Agrobacterium vitis. The disease is initiated at wound sites, such as graft unions and freeze injuries and limits grape production worldwide. There are no chemical or biological controls that have been successful to control this disease to date. A non-tumorigenic strain of A. vitis, F2/5, prevents crown gall on grapevines at wounds but also causes a necrosis that can be deleterious to graft union development and to root formation. A. vitis strain F2/5 is publically available from a number of laboratories throughout the world including from the laboratory of the Applicant's inventor, Dr. Thomas J. Burr (Department of Plant Pathology & Plant-Microbe Biology, New York State Agricultural Experiment Station, Cornell University, A104 Barton Hall 630 W. North Street, Geneva, N.Y. 14456-0462). As is disclosed herein, specific necrosis-minus derivatives of F2/5 that still retain biological control activities have been engineered. One mutant was generated by knocking out of a gene encoding phosphopantetheinyl transferase (PPTase), which is required for post-translational modification of polyketide synthase and non-ribosomal peptide synthase genes; another mutant was generated through the disruption of an aminotransferase; yet another mutant was targeted at an iron-response regulator. These mutations result in abolished necrosis but did not affect the ability of F2/5 to function as a biological control agent.

Such strains may be used in nurseries at grafting time (on graft unions and base of plants) as well as during field grafting of vines to prevent crown gall. Other uses are described herein.

The following examples are intended only to further illustrate the invention and are not intended to limit the scope of the invention as defined by the claims.

Example 1

Development of Agrobacterium vitis F2/5 Mutants:

Mutants of specific genes were generated through disruption with suicide vector pVIK165 (Kalogeraki et al. (1997) Gene 188: 69-75). Internal fragments of specific target genes were amplified from F2/5 genomic DNA with corresponding primer pairs. The specific genes that were mutated and primers used to amplify internal gene fragments are listed in the Table 2.

TABLE-US-00002 TABLE 2 Genes mutated and primers used in generation of the mutants size of Gene primer fragment targeted name primer sequence* amplified F-avi5813 avi5813-F: 5'-AAGAATTCTTGATCTCGCATCGCGCTG-3' 336 bp (PPTase) avi5813-R: 5'-AATCTAGATGCGCTGTCATGACCTGTTCTG-3' F-avi4329 avi4329-F: 5'-ACGAGCTCAGCTCGTCAGCGAGCAGCTG-3' 382 bp (amino- avi4329-R: 5'-AGTCTAGATGAACTCGTCATGGATCACCAG-3' transferase) F-avi0838 avi0838-F 5'-ACGAGCTCTGCCAAGGCCTGTGGTGTCTC-3' 224 bp (rirA) avi0838-R 5'-AGTCTAGATGTCGATCAACGGGCAATCGAC-3' *Restriction sites are underlined

XbaI and SacI restriction sites were introduced into forward and reverse primers respectively. PCR products were purified, digested and then ligated into suicide vector pVIK165 at XbaI and SacI sites. The constructs were transformed into E. coli strain S17-1/.lamda.pir by electroporation and transformants were selected on Luria-Bertani agar amended with kanamycin (50 .mu.g/ml). After verification by sequencing, the constructs were transferred to F2/5 via conjugal mating and gene disruptions occurred following single homologous recombination. Derivatives of F2/5 were selected on AB minimal medium amended with 10% mannitol and kanamycin (50 .mu.g/ml). Mutations were verified by PCR using primers derived from sequences of the F2/5 chromosome that flank the insertion site and sequence from the pVIK165 vector. Derivatives were then tested for their ability to prevent crown gall formation as described below.

Biological Control Assays:

F2/5 derivatives, .DELTA. F-avi5813, .DELTA. F-avi4329 and .DELTA. F-avi0838, which are all grape necrosis-minus, were tested for their ability to prevent crown gall on potted 1- to 2-month-old grapevines (V. vinifera cv. Chardonnay) in the greenhouse. Avi numbers correspond to gene homologs in the A. vitis S4 genome as described in the literature (see, for example, http://agro.vbi.vt.edu/public/index.html) (Slater et al., J. Bacteriol. 191:2501-11 (2009).). F2/5, derivatives of F2/5, and tumorigenic A. vitis strain CG49 were grown overnight on Potato Dextrose Agar (PDA) or PDA plus kanamycin and suspended in sterile distilled water and adjusted to OD.sub.600=0.1 (corresponding to about 10.sup.8 cells/ml). F2/5 and derivatives of F2/5 were mixed 1:1 with CG49, and 50 .mu.l of the mixed suspensions were inoculated on slice wounds made with a scalpel on woody trunks and/or green shoot tissues of the potted grapevines. Wounds were made just below a side shoot on woody tissue and below a bud on green shoots. After inoculum suspensions dried, the wound sites were wrapped with Parafilm. In general eight plants were inoculated for each treatment and experiments were repeated. The number of inoculation sites at which galls developed was recorded at least 6 weeks after inoculation for green shoots and 8 weeks for woody cutting tissue (Table 3 and FIG. 1). The necrosis-minus derivatives of F2/5 maintained their biological control activity.

TABLE-US-00003 TABLE 3 Biological control of crown gall in grapevine with F2/5 and F2/5 mutants Average Gall.sup.c/ gall size.sup.d Treatment Experiment.sup.b Inoculations (mm.sup.2) CG49 T-1 8/8 217.45 T-2 8/8 141.78 T-3 8/8 175.21 T-4 6/6 203.75 CG49 + F2/5 T-1 0/8 T-2 0/8 T-3 0/8 T-4 0/6 CG49 + .DELTA.avi0838 (rirA).sup.a T-1 0/8 T-2 2/8 >10 T-3 1/9 170.10 T-4 3/12 28.98 CG49 + .DELTA.avi4329 (amino- T-1 2/8 92.45 transferase) T-2 1/8 33.50 T-3 1/8 145.92 T-4 4/10 27.04 CG49 + .DELTA.avi5813 (PPTase) T-1 0/8 T-2 0/8 T-3 0/8 T-4 4/12 21.84 .sup.aavi numbers correspond to genes annotated in the A. vitis strain S4 genome sequence .sup.bT-1, experiment initiated on Dec. 6, 2011; T-2. initiated on Dec. 13, 2011; T-3, initiated on Dec. 19, 2011; T-4 initiated on Jan. 28, 2012. .sup.c"Gall" on the avi0838, avi4329 and avi5813 mutants treated vines may be callus development and not crown gall. .sup.dThe average gall size is calculated as total gall size/gall number instead of inoculation number.

Effect of F2/5 and Derivatives of F2/5 on Necrosis, Root and Callus Development:

Dormant grapevine cuttings were inoculated with strain F2/5 and derivatives of F2/5, .DELTA.F-avi5813), .DELTA.F-avi4329 and .DELTA.F-avi0838 by submersing basal ends of cuttings in water suspensions of the bacteria (about 10.sup.8 cfu/ml) for 4 hours prior to planting them in perlite to initiate callus and rooting. Data on necrosis, callus and root development and incidence of crown gall infection were collected 8 weeks after inoculation (Table 4 and FIG. 2). Cuttings treated with necrosis-minus derivatives produced enhanced roots and callus and lacked the significant necrosis seen on F2/5 treated cuttings.

TABLE-US-00004 TABLE 4 Effect of F2/5 and derivatives on root and callus development and on the level of necrosis on roots. Phenotypes were rates as 0-2 for necrosis (2 being more than 90% of roots having necrosis, 0-3 for root development with a 3 having the largest root mass and 0-3 for callus development with 3 equalling the largest amount of callus development at the cutting base. .DELTA.F- .DELTA.F-avi4329 Treatment/ avi0838 (amino- .DELTA.F-avi 5813 Response F2/5 Water (rirA) transferase) (PPTase) Necrosis (0-2) 2 0.1 0.3 0.2 0.3 Root 1.5 1.5 1.9 1.9 1.7 development (0-3) Callus 0.3 1.5 2.3 2.2 1.7 development (0-3)

On young stem explants, it was also shown that derivatives of F2/5 are necrosis-negative (FIG. 3).

SUMMARY

Crown gall is a significant disease of grapevines that often is initiated at grafts. Grafting may be done in the nursery (scion grafted onto rootstocks) or in the field when changing varieties of an established vineyard. Strain F2/5 is able to prevent the development of crown gall at wound sites on grapevines however is detrimental to graft take. As shown in FIG. 2, F2/5 is also detrimental to root formation. This is likely due to the fact that F2/5, like other A. vitis strains, initiates a necrosis of certain grape tissues.

Mutations in specific genes of strain F2/5 to generate F2/5 derivatives that no longer cause necrosis but have maintained biological control function were generated. These results have been repeatedly confirmed in the greenhouse as presented herein. In some cases small galls develop when the derivatives are applied together with a tumorigenic A. vitis strain (CG49) however the degree of galling is greatly reduced and, in most cases, no gall forms.

It was also observed that when dormant cuttings were treated with the mutants compared to the wildtype F2/5 strain, necrosis on roots was absent, an increased amount of roots were formed and increased callus at the base of the cuttings was noted. This is significant because the treatment is therefore beneficial to use even on grape cuttings that are not grafted. For example certain American and hybrid varieties are not grafted on rootstocks but rather grown on their own roots. Therefore treatment of such varieties with the F2/5 mutants may be advantageous in stimulating callusing and root development.

Example 2

Mutants that Display a Necrosis-Minus Phenotype and Remain Biological Control Positive:

Effects of F2/5 Necrosis-Minus Mutants on Grapevine Graft Take

Effects of F2/5 and the mutant .DELTA.F-avi5813 (PPTase), .DELTA.F-avi4329 (aminotransferase) and .DELTA.F-avi0838 (rirA) on graft take and root or shoot development were determined by treatment of grapevine cuttings (Cabernet Franc) with the bacterial suspensions. Bacterial strains were grown on PDA or PDA amended with kanamycin (50 .mu.g/ml) for 48 hr. The bacterial cells were suspended in water and adjusted to be OD=0.1. Dormant woody cuttings were cut with an omega graft tool. Following cutting the cut surfaces were dipped in bacterial suspensions and then fitted together and wrapped with Parafilm. The cuttings were planted in greenhouse potting mixture and kept moist until analyzed.

TABLE-US-00005 TABLE 5 Effects of A. vitis F2/5 and mutants on grapevine grafts Vines that formed Graft take Root Treatment shoots* Root formation on plants (%) necrosis Water 8/11 (72.7%) 7 with roots, all from rootstock 87% + 1 without roots F2/5 9/12 (75.0%) 8 with roots, all from graft sites 0 ++ 1 without roots CG49 5/12 (41.7%) 4 with roots, 3 from graft sites 20% ++ and 1 from rootstock, 1 without roots .DELTA.F-avi5813 10/14 (71.4) 10 with roots, 8 from rootstock 80% - (PPTase) and 2 from graft sites .DELTA.F-avi0838 10/19 (52.6%) 9 with roots, 6 from rootstock 66% - (rirA) and 3 from graft sites, 1 without root .DELTA.F-avi4329 9/14 (64.3%) 9 with roots, 7 from rootstock 77% - (aminotransferase) and 2 from graft sites F2/5 + CG49 7/18 (38.9%) 5 with roots, all from graft sites 0 ++ 2 without root .DELTA.F-avi5813 + 15/18 (83.3%) 14 with roots,12 from rootstock 85% - CG49 and 2 from graft sites, 1 without root .DELTA.F-avi0838 + 8/13 (61.5%) 8 with roots, 4 from rootstock 50% - CG49 and 4 from graft sites .DELTA.F-avi4329 + 8/13 (61.5%) 8 with roots, 4 from rootstock 50% - CG49 and 4 from graft sites -, no root necrosis; ++, heavy roots necrosis *Cuttings had single buds. Some buds did not grow and therefore the plants did not develop as shown in results.

The results indicate that the mutants improve graft take and root growth as compared to strain F2/5, tumorigenic strain CG49, or CG49 combined with F2/5 (FIG. 4 and Table 5). The roots from cuttings treated with the mutants grow with no or less necrosis and produce more roots (FIG. 4). Graft take was increased in mutant treatments as compared to F2/5, CG49 or CG49 and F2/5 (Table 5).

A. vitis F2/5 Genes Required for Biological Control but not for Necrosis: Avs Locus and F-avi5730

Avs Locus

The Avs locus as being unique to A. vitis strain F2/5 as no homologous loci present have been identified in other A. vitis strains using PCR screens. The Avs locus is approximately 12 kb in length and contains 10 ORFs that are flanked by two genes putatively encoding transposases (FIG. 5A) thereby suggesting horizontal gene transfer as a potential means of inquiry by F2/5. The DNA sequences and deduced amino acid sequences of the genes within Avs locus are shown in FIG. 5. Mutation of either gene P1391 encoding a siderophore synthase or gene P1396 encoding a siderophore ferrichrome-iron receptor resulted in loss of biological control activity by F2/5 (FIG. 5B). All of the genes listed in FIG. 5A are common in A. vitis strains except for those that reside in the Avs locus. Accordingly, one or more genes of the Avs locus is useful for generating new biological control strains by transferring it and expressing in non-tumorigenic A. vitis strains. Exemplary non-tumorigenic A. vitis strains are described in Burr et al. (1999) Plant Disease 83:102-107. Examples of such non-tumorigenic strains are nontumorigenic A. vitis from Vitis riparia (CG511, CG515, CG517, CG518, CG523, CG526, CG529, CG531, CG535, CG537, CG538, CG542, and CG544), nontumorigenic A. vitis from V. riparia (CG546, CG548, CG550, CG553, CG555, CG556, CG559, CG561, and CG565), nontumorigenic A. vitis from V. riparia (CG567, CG569, CG571, and CG572), nontumorigenic A. vitis, biocontrol for grape crown gall (F2/5), and nontumorigenic A. rhizogenes, biocontrol strain (K-84).

NRPS (F-avi5730)

A non-ribosomal peptide synthase (NRPS) gene (F-avi5730) in F2/5 as being required for biological control but not for necrosis was also identified. Avi5730 in F2/5 was mutagenized by gene disruption. The F-avi5730 (residing on chromosome II) mutant did not affect grape necrosis. Gene disruption also resulted in loss of biological control similar to results described above for disruption of the P1391 encoding the siderophore synthase and disruption of P1396 encoding the siderophore ferrichrome-iron receptor. Like Avs and its genes, the NRPS is useful for generating new biological control strains by transferring it and expressing in non-tumorigenic A. vitis strains.

Use

Exemplary uses of any of the engineered strains of Agrobacterium described herein are as follows.

Methods and rates of application of a necrosis-minus, non-tumorigenic Agrobacterium sp. such as a mutant derivative of A. vitis F2/5 which retains biological control of crown gall disease (for example, the PPTase, rirA, and aminotransferase gene mutants described herein) is accomplished according to standard practices known in the art. Typically, bacteria are applied as a dip solution (such as an inoculant) to any grapevine or grapevine component (grapevine component is a somatic embryo, a seed, a seedling, a scion, a rootstock, a cane, a cutting (e.g., a green cutting or a dormant cutting), a leaf, a stem, or a root in, for example, laboratories, nurseries, greenhouses, or in vineyards.

For purposes of the present invention, the bacteria are used as formulations or compositions. Such formulations or compositions contain one or more bacterium described herein and optionally a carrier. The carrier component can be a liquid or a solid material for delivering the formulation or composition to a desired site on a grapevine or grapevine component. Liquids suitable as carriers include water, and any liquid which will not affect the viability of the bacteria. Similarly, solid carriers can be virtually anything that is non-toxic to the bacterium. Non-limiting examples of solid carriers include peat, vermiculite, perlite, and soil or any other material used in commercial propagation of grape. Bacteria may be lyophilized or in powder form prepared according to standard methods known in the art.

Bacteria are administered according to standard practices for applying compositions to grapevine, grapevine component, or for application to a locus for planting a grapevine or a grapevine component such as soil. By the term "effective amount" or "amount effective for" is meant that minimum amount of a bacterial composition or bacterial formulation needed to at least reduce, or substantially eliminate crown gall disease on a grapevine or a grapevine component when compared to an untreated grapevine or grapevine component. Similarly, the terms refer to the minimum amount of bacteria needed to reduce necrosis or to promote callus or root development or both. The precise amount needed will vary in accordance with the particular bacterial composition used; the grapevine or grapevine component to be treated; and the environment in which the grapevine or grapevine component is located. The exact amount of bacteria in a composition needed can easily be determined by one having ordinary skill in the art given the teachings of the present specification. The examples herein show typical concentrations which will be needed to at least reduce crown gall disease as well as for promoting callus and root development.

In some embodiments, the active ingredient of a solid formulation is a necrosis-minus, biological control positive derivative of A. vitis F2/5 (e.g., the PPTase, rirA and aminotransferase gene mutants described herein or any combination of these derivatives) that includes not less than 1,000 million colony forming units (cfu) per gram of moist peat medium. For preparation, 100 grams of solid formulation when mixed with 1 gallon of water typically provides a suspension of approximately 2.6.times.10.sup.7 (cfu) per milliliter in a dip solution.

In other embodiments, the active ingredient of a liquid formulation is a necrosis-minus, biological control positive derivative of A. vitis F2/5 that includes not less than 1,000 million cfu per milliliter of water.

Plants are typically dipped in a solution of a necrosis-minus, biological control positive derivative of Agrobacterium sp. such as any one of the mutant derivative of A. vitis F2/5 described herein. Alternatively, the formulation or composition is sprayed on a plant; for example, by spraying to graft surfaces during grafting. In other applications, the formulation or composition is applied to a locus where the plant is growing or to be planted.

Compositions and formulations are applied to grapevine or grapevine components as needed according to standard viticultural methods. The compositions can be applied as needed to any plant wound or damaged plant tissue that allows entry of a tumorigenic A. vitis sp. Typically, such applications may be sprayed or painted on the plant. In other situations, applications of the compositions or formulations would be to grafts at time of grafting. This would be most typically done in a nursery as well as in field grafting. An exemplary grafting situation is where a grower changes varieties in the vineyard by grafting to a current vine, for example, changing from Riesling to Chardonnay by field grafting. For example, for grafting applications, grapevine or grapevine components should be treated with the bacteria after cutting or other handling operations that damage plant tissue and before and after cold storage. Bare rootstock or scion material should be dipped or sprayed with the formulation solution until all root surfaces or scion surfaces or the stem above the graft union are completely wet, or spray these tissues to runoff.

Grapevines or grapevine components may also be treated with a formulation or composition before and after cold storage or at both times.

Because the strains described herein also enhance callus and rooting of dormant cuttings, the bacteria useful in the nursery for rooting purposes. This is beneficial on grafted and non-grafted varieties. For example, many of grape hybrids and labrusca varieties are not grafted but callusing and rooting is important for regeneration. Another exemplary use involves a grower to dip rooted and grafted (or non-grafted) vines obtained from a nursery in a formulation or composition prior to planting. Such treatments would enhance growth in the vineyard.

Any of the genes described herein are useful for engineering A. vitis strains (tumorigenic and non-tumorgenic alike) to necrosis minus phenotype and crown gall positive phenotype. Methods for engineering such strains are according to standard methods in the art including those described herein.

Other Embodiments

While the invention has been described in connection with specific embodiments thereof, it will be understood that it is capable of further modifications and this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure come within known or customary practice within the art to which the invention pertains and may be applied to the essential features hereinbefore set forth.

All publications are herein incorporated by reference in their entirety to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety.

SEQUENCE LISTINGS

1

371735DNAA. vitis strain F2/5misc_feature(1)..(735)avi5813 1atgagccagc gggccaataa tcaggtggaa atatactcct catgccctgt gcctccgctt 60ttcgaggcct tcatctcgca tcgcgctgtt tgcttccgtc acaatgattt tacgcccgaa 120gcggcgattg aacttggcgt gcccctgcca gaaagcatgg gaaaggcggt cgccaagcgc 180aaggcggaat atgtgggcgg acggttctgc gccatggagg cgattgtggc gcaaaccggc 240cagcctgccg cacccgttac agcgggaccg cgtggcgaac cggtctggcc gtcagggctg 300gtcggctcga ttacccatac gcacgggttt gctgcggcag ccgttgccga tgcagctcga 360tttcgcagcc ttggcatgga tacagaacag gtcatgacag cgcaggtcat gggcaatgtc 420cgagagcgga tctgcggtcc ggaagaccgg tttggggcca gcagctctct tttgccggaa 480cttcatacca ccctggtgtt ttctgccaag gaaagcctgt tcaaatgtct ttatccattg 540gttgaaaaaa tgttctggtt cgaagacgcg ctgatccgga tcgatccgga tcgggatggt 600ctgtttaccg ccgaattgct gtcacgcctc catgtggaat ttccggcggg aacagtgatc 660gaaggacgct tttgcctgac gccgggtctg gttcataccg gcatcagcct tgcgaaagat 720gaggctgcgt tataa 7352244PRTA. vitis strain F2/5MISC_FEATURE(1)..(244)avi5813 2Met Ser Gln Arg Ala Asn Asn Gln Val Glu Ile Tyr Ser Ser Cys Pro 1 5 10 15 Val Pro Pro Leu Phe Glu Ala Phe Ile Ser His Arg Ala Val Cys Phe 20 25 30 Arg His Asn Asp Phe Thr Pro Glu Ala Ala Ile Glu Leu Gly Val Pro 35 40 45 Leu Pro Glu Ser Met Gly Lys Ala Val Ala Lys Arg Lys Ala Glu Tyr 50 55 60 Val Gly Gly Arg Phe Cys Ala Met Glu Ala Ile Val Ala Gln Thr Gly 65 70 75 80 Gln Pro Ala Ala Pro Val Thr Ala Gly Pro Arg Gly Glu Pro Val Trp 85 90 95 Pro Ser Gly Leu Val Gly Ser Ile Thr His Thr His Gly Phe Ala Ala 100 105 110 Ala Ala Val Ala Asp Ala Ala Arg Phe Arg Ser Leu Gly Met Asp Thr 115 120 125 Glu Gln Val Met Thr Ala Gln Val Met Gly Asn Val Arg Glu Arg Ile 130 135 140 Cys Gly Pro Glu Asp Arg Phe Gly Ala Ser Ser Ser Leu Leu Pro Glu 145 150 155 160 Leu His Thr Thr Leu Val Phe Ser Ala Lys Glu Ser Leu Phe Lys Cys 165 170 175 Leu Tyr Pro Leu Val Glu Lys Met Phe Trp Phe Glu Asp Ala Leu Ile 180 185 190 Arg Ile Asp Pro Asp Arg Asp Gly Leu Phe Thr Ala Glu Leu Leu Ser 195 200 205 Arg Leu His Val Glu Phe Pro Ala Gly Thr Val Ile Glu Gly Arg Phe 210 215 220 Cys Leu Thr Pro Gly Leu Val His Thr Gly Ile Ser Leu Ala Lys Asp 225 230 235 240 Glu Ala Ala Leu 31368DNAA. vitis strain F2/5misc_feature(1)..(1368)avi4329 3atgaatgaga acaagcgtca ggatctgctg gcccgtatgc gtggcgtaca aaaggatgtg 60gaccggagcc gggccagccg cagccagcag agcgtgtcgc gcagccagcc aggttttgcc 120gaattgcctg aatataagca ggtggtgatg cagaagcttg tcagcgagca actgaacatg 180cccaatccgt tttaccgggc gcatgagagc gcctccgggg caacggctga tatcgacggg 240cgcgcctatg ataattttgc ctcctatgat tatctcggct tgaacagtga tccacgcatt 300cgcgatgccg ccatggcggc catcgaccag ttcggtatct cggcctccgc cagccggctt 360gttgccggtg agcgcaccat ccatgccgag ctggaaaagg cctttgccaa aaattaccag 420accgaggacg ccatctgttt cgtcagcggc tatctcacca atgtcacgac catcggcagc 480ttgatggggc cgaaagacct ggtgatccat gacgagttca ttcacaacag cgccctgacg 540ggcatcaagc tgtcgggtgc caatcgccgc ttcttcaagc ataatgacat ggcggacctg 600gaccgtattc ttgcgagcct cgcccccttg catgaacgaa tcctggtgat ctccgagggc 660attttctcca tggatggaga cgttgccgat ctgccgggcc ttcttgcatt gaaaaagcag 720tataatttct ggctgatgat ggacgaggcc cattctctgg gcgtacttgg acagcgtggc 780cacggcattt tcgaacattt caatctcgat ccagccgatg tcgatatctg gatggggacg 840ctgtcgaaaa ccacctgtag ctgcggcggc tatgttgccg gcagtgaagc gctgatcacg 900ctgttgaagg cgcaggccgg tggtttcgtt tatagtgtcg gcttggcacc agcgctggcg 960gctgcggcca tcgccagcct gtcggtgctg gacgaggagc cggaccgggt agaggctttg 1020cgtcgcaaca gccagctttt cctggagcaa gccaagctac gggggctgga taccggtctg 1080agcgaaggtt tctcggtggt gcctgtgatc gttgccgatt cggtgcgcgc tgtgcagctt 1140tccaatgaat tgttcgaggc aggcatcaat gcgctgccga tcatctatcc cgcagtaccg 1200gaaggtctgg cacgtctgcg gttcttcatc accagcgccc acacgcccga ccagattacc 1260cgcagcgtgg acaaggtggc ggacattctg gatcggctga aggccgagaa tttcggcatg 1320ggctcgatgg atgtccagaa ggtcatgctg caattggcgc agcgctga 13684455PRTA. vitis strain F2/5MISC_FEATURE(1)..(455)avi4329 4Met Asn Glu Asn Lys Arg Gln Asp Leu Leu Ala Arg Met Arg Gly Val 1 5 10 15 Gln Lys Asp Val Asp Arg Ser Arg Ala Ser Arg Ser Gln Gln Ser Val 20 25 30 Ser Arg Ser Gln Pro Gly Phe Ala Glu Leu Pro Glu Tyr Lys Gln Val 35 40 45 Val Met Gln Lys Leu Val Ser Glu Gln Leu Asn Met Pro Asn Pro Phe 50 55 60 Tyr Arg Ala His Glu Ser Ala Ser Gly Ala Thr Ala Asp Ile Asp Gly 65 70 75 80 Arg Ala Tyr Asp Asn Phe Ala Ser Tyr Asp Tyr Leu Gly Leu Asn Ser 85 90 95 Asp Pro Arg Ile Arg Asp Ala Ala Met Ala Ala Ile Asp Gln Phe Gly 100 105 110 Ile Ser Ala Ser Ala Ser Arg Leu Val Ala Gly Glu Arg Thr Ile His 115 120 125 Ala Glu Leu Glu Lys Ala Phe Ala Lys Asn Tyr Gln Thr Glu Asp Ala 130 135 140 Ile Cys Phe Val Ser Gly Tyr Leu Thr Asn Val Thr Thr Ile Gly Ser 145 150 155 160 Leu Met Gly Pro Lys Asp Leu Val Ile His Asp Glu Phe Ile His Asn 165 170 175 Ser Ala Leu Thr Gly Ile Lys Leu Ser Gly Ala Asn Arg Arg Phe Phe 180 185 190 Lys His Asn Asp Met Ala Asp Leu Asp Arg Ile Leu Ala Ser Leu Ala 195 200 205 Pro Leu His Glu Arg Ile Leu Val Ile Ser Glu Gly Ile Phe Ser Met 210 215 220 Asp Gly Asp Val Ala Asp Leu Pro Gly Leu Leu Ala Leu Lys Lys Gln 225 230 235 240 Tyr Asn Phe Trp Leu Met Met Asp Glu Ala His Ser Leu Gly Val Leu 245 250 255 Gly Gln Arg Gly His Gly Ile Phe Glu His Phe Asn Leu Asp Pro Ala 260 265 270 Asp Val Asp Ile Trp Met Gly Thr Leu Ser Lys Thr Thr Cys Ser Cys 275 280 285 Gly Gly Tyr Val Ala Gly Ser Glu Ala Leu Ile Thr Leu Leu Lys Ala 290 295 300 Gln Ala Gly Gly Phe Val Tyr Ser Val Gly Leu Ala Pro Ala Leu Ala 305 310 315 320 Ala Ala Ala Ile Ala Ser Leu Ser Val Leu Asp Glu Glu Pro Asp Arg 325 330 335 Val Glu Ala Leu Arg Arg Asn Ser Gln Leu Phe Leu Glu Gln Ala Lys 340 345 350 Leu Arg Gly Leu Asp Thr Gly Leu Ser Glu Gly Phe Ser Val Val Pro 355 360 365 Val Ile Val Ala Asp Ser Val Arg Ala Val Gln Leu Ser Asn Glu Leu 370 375 380 Phe Glu Ala Gly Ile Asn Ala Leu Pro Ile Ile Tyr Pro Ala Val Pro 385 390 395 400 Glu Gly Leu Ala Arg Leu Arg Phe Phe Ile Thr Ser Ala His Thr Pro 405 410 415 Asp Gln Ile Thr Arg Ser Val Asp Lys Val Ala Asp Ile Leu Asp Arg 420 425 430 Leu Lys Ala Glu Asn Phe Gly Met Gly Ser Met Asp Val Gln Lys Val 435 440 445 Met Leu Gln Leu Ala Gln Arg 450 455 5471DNAA. vitis strain F2/5misc_feature(1)..(471)avi0838 5atgcgcttga cgaagcagac caattacgcg gtacgcattc tgatgtattg cgcggcgaac 60aaggaccacc tgagccgtat cccggaaatt gccaaggcct atggcgtctc cgaactgttc 120ctgttcaaaa tccttcagcc gctcaacaag gcgggtctgg tggaaaccgt gcgtggccgc 180aatggcggcg tgcgcctagg tcgtgcgcct gaaaagatca gcctgtttga cgtggtcaag 240gtcacggaag acagctttgc gatggccgaa tgcttcgagg acgatggcga ggtcgattgc 300ccgttgatcg acagctgcgg cctgaattcg gcgctgcgca aggcgctcaa cgctttcttc 360gcggtgctgg cggaatattc catcgatgat ctggtcaagg cccgcccgca gatcaacttt 420ttgctgggta tcgaggatct gcctcgtctg gcggcggcac cggctgcctg a 4716156PRTA. vitis strain F2/5MISC_FEATURE(1)..(156)avi0838 6Met Arg Leu Thr Lys Gln Thr Asn Tyr Ala Val Arg Ile Leu Met Tyr 1 5 10 15 Cys Ala Ala Asn Lys Asp His Leu Ser Arg Ile Pro Glu Ile Ala Lys 20 25 30 Ala Tyr Gly Val Ser Glu Leu Phe Leu Phe Lys Ile Leu Gln Pro Leu 35 40 45 Asn Lys Ala Gly Leu Val Glu Thr Val Arg Gly Arg Asn Gly Gly Val 50 55 60 Arg Leu Gly Arg Ala Pro Glu Lys Ile Ser Leu Phe Asp Val Val Lys 65 70 75 80 Val Thr Glu Asp Ser Phe Ala Met Ala Glu Cys Phe Glu Asp Asp Gly 85 90 95 Glu Val Asp Cys Pro Leu Ile Asp Ser Cys Gly Leu Asn Ser Ala Leu 100 105 110 Arg Lys Ala Leu Asn Ala Phe Phe Ala Val Leu Ala Glu Tyr Ser Ile 115 120 125 Asp Asp Leu Val Lys Ala Arg Pro Gln Ile Asn Phe Leu Leu Gly Ile 130 135 140 Glu Asp Leu Pro Arg Leu Ala Ala Ala Pro Ala Ala 145 150 155 7741DNAA. vitis strain F2/5misc_feature(1)..(741)avi4374 7gtgagtgtca atcatctcat acagtttttg gctgtatctc agggatgccg aagtcgcgag 60gagctgatcc tagaattgga gaaactcctc gaattttata aattcgatta ttacggcctc 120gtgcgcagtc ccaaacctga ccaaaacccc atgtcgctgg tgcttgcagg acgctggccg 180gaaaaatggc cgcaggtcta tatcacgaaa aaattcgtgc tgatcgaccc tgccattcgc 240tatctggcgc aagcgcaacg gccgttccgt tggagcgaaa cgctgacggc attcagccag 300gacccacatt tcaaacgcat gcagcggatg atgatggatg cccagcgctt tggtctcgag 360gaaggctata tcttccccat ccacgggcgg ggcggattgc tggccaacat gaccattggc 420gggagaccgg tggaactgac cccgattgag atcatgctgt tcgacacggt ggcgaaatgc 480gccttctggc ggctggcgga aatcaatggc gaagacgaac tgctgtcgat ggtgcaaaaa 540gtcgatgtgc gcctgacccg acgcgagctt gaaattctca attatctcgg ggagggcatg 600acctcgaacg agatgagcaa attgctcgag atttcgaacc acaccgtcga ttggtacgtc 660aacgaattgc aggataagtt gaacgccaag aatcggcaac atatggtagc aattgcttat 720cgactcggcc tgatcagctg a 7418246PRTA. vitis strain F2/5MISC_FEATURE(1)..(246)avi4374 8Met Ser Val Asn His Leu Ile Gln Phe Leu Ala Val Ser Gln Gly Cys 1 5 10 15 Arg Ser Arg Glu Glu Leu Ile Leu Glu Leu Glu Lys Leu Leu Glu Phe 20 25 30 Tyr Lys Phe Asp Tyr Tyr Gly Leu Val Arg Ser Pro Lys Pro Asp Gln 35 40 45 Asn Pro Met Ser Leu Val Leu Ala Gly Arg Trp Pro Glu Lys Trp Pro 50 55 60 Gln Val Tyr Ile Thr Lys Lys Phe Val Leu Ile Asp Pro Ala Ile Arg 65 70 75 80 Tyr Leu Ala Gln Ala Gln Arg Pro Phe Arg Trp Ser Glu Thr Leu Thr 85 90 95 Ala Phe Ser Gln Asp Pro His Phe Lys Arg Met Gln Arg Met Met Met 100 105 110 Asp Ala Gln Arg Phe Gly Leu Glu Glu Gly Tyr Ile Phe Pro Ile His 115 120 125 Gly Arg Gly Gly Leu Leu Ala Asn Met Thr Ile Gly Gly Arg Pro Val 130 135 140 Glu Leu Thr Pro Ile Glu Ile Met Leu Phe Asp Thr Val Ala Lys Cys 145 150 155 160 Ala Phe Trp Arg Leu Ala Glu Ile Asn Gly Glu Asp Glu Leu Leu Ser 165 170 175 Met Val Gln Lys Val Asp Val Arg Leu Thr Arg Arg Glu Leu Glu Ile 180 185 190 Leu Asn Tyr Leu Gly Glu Gly Met Thr Ser Asn Glu Met Ser Lys Leu 195 200 205 Leu Glu Ile Ser Asn His Thr Val Asp Trp Tyr Val Asn Glu Leu Gln 210 215 220 Asp Lys Leu Asn Ala Lys Asn Arg Gln His Met Val Ala Ile Ala Tyr 225 230 235 240 Arg Leu Gly Leu Ile Ser 245 9633DNAA. vitis strain F2/5misc_feature(1)..(633)avi1696 9atgagaaatc cagttgatac cgccatggct ctggtgccta tggttgtgga gcagaccaat 60cgcggcgaac ggtcctacga catctactcg cgtctgttga aggagcgcat cattttcctg 120accggaccgg ttgaggatca tatggcgtcg cttgtctgcg cccagctgct gtttctggaa 180gcggaaaatc cgaagaagga aatcgcgatc tacatcaatt ctcccggcgg cgtcgtgact 240gccggcatgg cgatctacga tacgatgcag ttcatccgtc cggccgtctc gacgctgtgc 300gtcggccagg ccgcctcgat ggggtcgctg ctgctggcgg ccggcgaaaa gggcatgcgc 360tttgcgaccc ccaacgcccg catcatggtg catcagccgt ccggcggttt ccaggggcag 420gcctccgaca tcgagcggca tgcccgcgac atcatcaaga tgaagcgtcg cttgaacgag 480gtttatgtca agcacactgg acgcacgctg gaagaagtcg agcacacgct tgaccgcgac 540cacttcatgg aatccacgga agctaaggat tggggtctga tcgacaagat cctgacgacg 600cgctccgaaa ttgaaggggt aacgccttct tga 63310210PRTA. vitis strain F2/5MISC_FEATURE(1)..(210)avi1696 10Met Arg Asn Pro Val Asp Thr Ala Met Ala Leu Val Pro Met Val Val 1 5 10 15 Glu Gln Thr Asn Arg Gly Glu Arg Ser Tyr Asp Ile Tyr Ser Arg Leu 20 25 30 Leu Lys Glu Arg Ile Ile Phe Leu Thr Gly Pro Val Glu Asp His Met 35 40 45 Ala Ser Leu Val Cys Ala Gln Leu Leu Phe Leu Glu Ala Glu Asn Pro 50 55 60 Lys Lys Glu Ile Ala Ile Tyr Ile Asn Ser Pro Gly Gly Val Val Thr 65 70 75 80 Ala Gly Met Ala Ile Tyr Asp Thr Met Gln Phe Ile Arg Pro Ala Val 85 90 95 Ser Thr Leu Cys Val Gly Gln Ala Ala Ser Met Gly Ser Leu Leu Leu 100 105 110 Ala Ala Gly Glu Lys Gly Met Arg Phe Ala Thr Pro Asn Ala Arg Ile 115 120 125 Met Val His Gln Pro Ser Gly Gly Phe Gln Gly Gln Ala Ser Asp Ile 130 135 140 Glu Arg His Ala Arg Asp Ile Ile Lys Met Lys Arg Arg Leu Asn Glu 145 150 155 160 Val Tyr Val Lys His Thr Gly Arg Thr Leu Glu Glu Val Glu His Thr 165 170 175 Leu Asp Arg Asp His Phe Met Glu Ser Thr Glu Ala Lys Asp Trp Gly 180 185 190 Leu Ile Asp Lys Ile Leu Thr Thr Arg Ser Glu Ile Glu Gly Val Thr 195 200 205 Pro Ser 210 114005DNAA. vitis strain F2/5misc_feature(1)..(4005)avi3342 11atgtcttttg taggcagaca tgagcaggtt tttccggttt ctcttgcgca ggcaggcttg 60tgggtcaaac aaaaggtagc tcccgccgat ctgagctttg tccttgctga atccatcgaa 120attcacggtc ccgttcatcc aggactgttc tgccaggcgt tgcgccgctt gtcagatgat 180gttgctgtca cacggtctcg catcaaggaa atcgaagggc agccacatca ggttgtcatg 240gcggcctatt gcggcgtctt tgacgtgatc gatttcagcg gtgctgacaa tcctggcgca 300agcgccatgg actggatgcg caagcagatg tctaagccgc tcgatcttgc caatgacaat 360ctctgggggt cttcactttt gaagctcggg gccacagaat gggtctggta tcattgggca 420catcatatca tcatggatgg gttctctggc ggcttgctgg cccggcggct ggccgatatt 480tattcggcgc tggcccaggg caaccagccg gagccctatg attgcggttc tccgcaggaa 540ttgctggagc ttgagcgcac ctaccgcgat tcggtgcatt tccagcgcga caaggcctat 600tggtcggagc agatgaaggg tctgccggag ccggtgacct tgatgaagaa aaaaggcgag 660ccatccggtg gcctgttgcg ccatacgacg gtcatcgatc gccagacggt caaggcgctt 720gctgagatca gccgtggctt tggcgccagc gtgccacagg ccttgattgc ccttgtcgcg 780gcctattacg ccaaggcgac cgactgcgaa gagctgacca tggtcaccat ggtgacggcc 840cggatcagcc agacgatgcg ccgcattccg ggcatgaccg ccaatgcggt tcctctgcgg 900ttttcgatca cgccggacct gtcctggcgc gaattgaccg gtcaggtttc ccagcagatg 960agccgggcgc tgcgctatca gcgctatcgc tatgaggata ttcgccgcga tctcggcatg 1020gtccgccagg atgcgcagat tgcctggctg ggcgtcaata tcgagccttt cgactacgat 1080ctacgctttg atggacagcc aaccaccgtg cacaaccttt cgaatggcac gatgacggat 1140ttcaccatct tcgcctatga ccgtggtgac aatggcgacc tgcgcatcga ttttgatgcc 1200aatccggcgc tctatacgct ggaagagctg gccgatcacg aggcgcggtt cacccgcatg 1260ttgagagaga tcctcgcgac gcctgaacag cccctgcgcg atttcagcct gctgtcgaag 1320tgggagcggc aggaaattct caccgactgg aacgatacgt cccataagct gccggatcag 1380acctggccgg aattgttccg ggcgcaggcg gcacgcacgc cggatgcggt agcgctatcc 1440tttggcggtc gccagatgac ctatggtgag ctggatgccg cctccgatca tctggctggc 1500tatctgatgg aaaagggcgc cgtacccggt tctctggtgg ctgtggctgt gccgcggtct 1560gaaaacatgg ttgtggcgct gctggcagtg ctcaaaagcg gtgccgccta tctaccgctc 1620gatccggctg atccagcctc gcgggtggcg atgatcctgg aggatgcgca gccagcctgc 1680gttatcacca ctgaggaagt ggcgggcaac ctgccggatg ccaccgacaa cctgatcttc 1740ctcgacaagc ccctcgagcg acagggacgc gacctgccga aggggccatc gctgagcgac 1800acggcctata tcatcttcac gtccggttcg

accggtcggc ccaagggcgt ggaaattcca 1860catcgcggcc tgatgaactt cctgttgtcg atgcaggatc tgttgaaact ggacagcgaa 1920gaccggttgc tggctgtgac gacgatttcc ttcgatattg cagcacttga gctttatctg 1980cccttgctgg caggcgcgcg cacggtgatc gcgctgcgct cggaagtgcg cgacccggcg 2040gtgctgcatg ggttgatccg cagtgcgggt atcaccatca tgcaggcgac gccgtctttg 2100tggcgggcct tgctggccga tcatcatgag ggactgacgg gcttgcgcag tcttgtcggt 2160ggtgaggcgc ttcccgccga tctcgcccat aagatggcgc ggctcggcca cccggttctc 2220aatgtctacg gaccgacgga aaccacgatc tggtccacca acatgccgct tttgggcagc 2280gacctcgaca gtgcgccaat tggccggcca atctggaaca cccgcgttta tgtcctcgat 2340cggcattgcc agccagtgcc gcccggcttt atcggtgagc tgtatatcgg tggtgcgggc 2400gtggcgaagg gctacctgaa ccgcccggac ctgacggcgg aaaaattcat ggctgatcct 2460ttcgcaggcg aaggcgagcg gatctaccgg accggcgacc tggtgcgctg gcggcgcgat 2520ggcgtgctgg attatctcgg ccgcaacgac caccagatca agattcgtgg attccgggtg 2580gagccgggcg aaatcgaggc ggcgctttcc gccctgccgc aggtgcgtga agcggtggtt 2640attctgcgcg atgatccggg ccgcgaaaag cgactggttg cctatgttgt ccccagagag 2700ggtgcggtag gcgagggtgc ctcgctggat gctgctgatc tttcggagcg gctaggcaag 2760gttctgcccg ttcacatgat cccggcggcc tatgtggtgc tggacgctat tccgttgaat 2820tccaatggca agaccgaccg gcacgcgctg ccggtaccgc aatggacggt gacggaaggc 2880atagccctgc cggggaccga tgctgaaaag cggcttgccg ccttgtggtg cgagatcctt 2940ggtctggagc agatcggcat tcacgacagt ttcttcgtgc tgggtggtga ctcgctggct 3000gccgctggaa tgatttccgc cgttcgcagc cgattgaagg gcgaaattcc cctcggtgcc 3060gtgttcgaaa caccaaccat tgccgccttg gccgtgcatc tggatgaggc aagctcgggt 3120tcaccactga ttgaaccggt tctggccatc cgcgccaagg gcgagcgccc gccgctgttc 3180tgcatccatc cggtgctggg cttgggctgg agcttcttct cgctcgcaca acatttgagc 3240gaggatgtcc cggtttacgc cctgcaatcg gacgggttgc atgacctcgc cgccctgccg 3300cgctctatcg aggatatggc tgcgctctat gtgcagcgga ttcgcaagat ccagccgcag 3360gggccatatc atctgctggg ctggtcattg ggcgggctga ttgcccatga aatgacccgg 3420caattgcagg cagaaggcca ggctatcgcg ttcctgggca tgatggacag ctaccatttc 3480aaacctgctg cccaattgca ggacgatgcg acgctggcgc gggccgccct cggtttcctc 3540ggctttgatg aaaaggcagc gggcgacaag ccatcgctgg ccgagcttgg cgatttcgtg 3600ctgaaaatgt tcgatacgga caaccatgtc ctgctggaac aggttcacca gttcgatccc 3660gagttcgtcg agcgcgccaa ggcaaccatc cttcacaatc tggagattgc ccagcgcttt 3720acaccgggca agatcgatgc cgatgtgcat ttcttccgcg ccgaaccaag tgcgggcagc 3780gatgcgctga acaccatcct caactacgat gcggaaacct ggttgccgca tgtggagggc 3840agggtttacc tgcgcgacat gacctgcaac caccacgaca tgctgaatat cgaaccggca 3900tcccatatca gcgccgtcgt tcaagcagag ttgctgcgtg aattcctggt cctgcgtcgg 3960ccccagactg aggtcaagat tgagcgtgtg tcgcggtttg cctaa 4005121334PRTA. vitis strain F2/5MISC_FEATURE(1)..(1334)avi3342 12Met Ser Phe Val Gly Arg His Glu Gln Val Phe Pro Val Ser Leu Ala 1 5 10 15 Gln Ala Gly Leu Trp Val Lys Gln Lys Val Ala Pro Ala Asp Leu Ser 20 25 30 Phe Val Leu Ala Glu Ser Ile Glu Ile His Gly Pro Val His Pro Gly 35 40 45 Leu Phe Cys Gln Ala Leu Arg Arg Leu Ser Asp Asp Val Ala Val Thr 50 55 60 Arg Ser Arg Ile Lys Glu Ile Glu Gly Gln Pro His Gln Val Val Met 65 70 75 80 Ala Ala Tyr Cys Gly Val Phe Asp Val Ile Asp Phe Ser Gly Ala Asp 85 90 95 Asn Pro Gly Ala Ser Ala Met Asp Trp Met Arg Lys Gln Met Ser Lys 100 105 110 Pro Leu Asp Leu Ala Asn Asp Asn Leu Trp Gly Ser Ser Leu Leu Lys 115 120 125 Leu Gly Ala Thr Glu Trp Val Trp Tyr His Trp Ala His His Ile Ile 130 135 140 Met Asp Gly Phe Ser Gly Gly Leu Leu Ala Arg Arg Leu Ala Asp Ile 145 150 155 160 Tyr Ser Ala Leu Ala Gln Gly Asn Gln Pro Glu Pro Tyr Asp Cys Gly 165 170 175 Ser Pro Gln Glu Leu Leu Glu Leu Glu Arg Thr Tyr Arg Asp Ser Val 180 185 190 His Phe Gln Arg Asp Lys Ala Tyr Trp Ser Glu Gln Met Lys Gly Leu 195 200 205 Pro Glu Pro Val Thr Leu Met Lys Lys Lys Gly Glu Pro Ser Gly Gly 210 215 220 Leu Leu Arg His Thr Thr Val Ile Asp Arg Gln Thr Val Lys Ala Leu 225 230 235 240 Ala Glu Ile Ser Arg Gly Phe Gly Ala Ser Val Pro Gln Ala Leu Ile 245 250 255 Ala Leu Val Ala Ala Tyr Tyr Ala Lys Ala Thr Asp Cys Glu Glu Leu 260 265 270 Thr Met Val Thr Met Val Thr Ala Arg Ile Ser Gln Thr Met Arg Arg 275 280 285 Ile Pro Gly Met Thr Ala Asn Ala Val Pro Leu Arg Phe Ser Ile Thr 290 295 300 Pro Asp Leu Ser Trp Arg Glu Leu Thr Gly Gln Val Ser Gln Gln Met 305 310 315 320 Ser Arg Ala Leu Arg Tyr Gln Arg Tyr Arg Tyr Glu Asp Ile Arg Arg 325 330 335 Asp Leu Gly Met Val Arg Gln Asp Ala Gln Ile Ala Trp Leu Gly Val 340 345 350 Asn Ile Glu Pro Phe Asp Tyr Asp Leu Arg Phe Asp Gly Gln Pro Thr 355 360 365 Thr Val His Asn Leu Ser Asn Gly Thr Met Thr Asp Phe Thr Ile Phe 370 375 380 Ala Tyr Asp Arg Gly Asp Asn Gly Asp Leu Arg Ile Asp Phe Asp Ala 385 390 395 400 Asn Pro Ala Leu Tyr Thr Leu Glu Glu Leu Ala Asp His Glu Ala Arg 405 410 415 Phe Thr Arg Met Leu Arg Glu Ile Leu Ala Thr Pro Glu Gln Pro Leu 420 425 430 Arg Asp Phe Ser Leu Leu Ser Lys Trp Glu Arg Gln Glu Ile Leu Thr 435 440 445 Asp Trp Asn Asp Thr Ser His Lys Leu Pro Asp Gln Thr Trp Pro Glu 450 455 460 Leu Phe Arg Ala Gln Ala Ala Arg Thr Pro Asp Ala Val Ala Leu Ser 465 470 475 480 Phe Gly Gly Arg Gln Met Thr Tyr Gly Glu Leu Asp Ala Ala Ser Asp 485 490 495 His Leu Ala Gly Tyr Leu Met Glu Lys Gly Ala Val Pro Gly Ser Leu 500 505 510 Val Ala Val Ala Val Pro Arg Ser Glu Asn Met Val Val Ala Leu Leu 515 520 525 Ala Val Leu Lys Ser Gly Ala Ala Tyr Leu Pro Leu Asp Pro Ala Asp 530 535 540 Pro Ala Ser Arg Val Ala Met Ile Leu Glu Asp Ala Gln Pro Ala Cys 545 550 555 560 Val Ile Thr Thr Glu Glu Val Ala Gly Asn Leu Pro Asp Ala Thr Asp 565 570 575 Asn Leu Ile Phe Leu Asp Lys Pro Leu Glu Arg Gln Gly Arg Asp Leu 580 585 590 Pro Lys Gly Pro Ser Leu Ser Asp Thr Ala Tyr Ile Ile Phe Thr Ser 595 600 605 Gly Ser Thr Gly Arg Pro Lys Gly Val Glu Ile Pro His Arg Gly Leu 610 615 620 Met Asn Phe Leu Leu Ser Met Gln Asp Leu Leu Lys Leu Asp Ser Glu 625 630 635 640 Asp Arg Leu Leu Ala Val Thr Thr Ile Ser Phe Asp Ile Ala Ala Leu 645 650 655 Glu Leu Tyr Leu Pro Leu Leu Ala Gly Ala Arg Thr Val Ile Ala Leu 660 665 670 Arg Ser Glu Val Arg Asp Pro Ala Val Leu His Gly Leu Ile Arg Ser 675 680 685 Ala Gly Ile Thr Ile Met Gln Ala Thr Pro Ser Leu Trp Arg Ala Leu 690 695 700 Leu Ala Asp His His Glu Gly Leu Thr Gly Leu Arg Ser Leu Val Gly 705 710 715 720 Gly Glu Ala Leu Pro Ala Asp Leu Ala His Lys Met Ala Arg Leu Gly 725 730 735 His Pro Val Leu Asn Val Tyr Gly Pro Thr Glu Thr Thr Ile Trp Ser 740 745 750 Thr Asn Met Pro Leu Leu Gly Ser Asp Leu Asp Ser Ala Pro Ile Gly 755 760 765 Arg Pro Ile Trp Asn Thr Arg Val Tyr Val Leu Asp Arg His Cys Gln 770 775 780 Pro Val Pro Pro Gly Phe Ile Gly Glu Leu Tyr Ile Gly Gly Ala Gly 785 790 795 800 Val Ala Lys Gly Tyr Leu Asn Arg Pro Asp Leu Thr Ala Glu Lys Phe 805 810 815 Met Ala Asp Pro Phe Ala Gly Glu Gly Glu Arg Ile Tyr Arg Thr Gly 820 825 830 Asp Leu Val Arg Trp Arg Arg Asp Gly Val Leu Asp Tyr Leu Gly Arg 835 840 845 Asn Asp His Gln Ile Lys Ile Arg Gly Phe Arg Val Glu Pro Gly Glu 850 855 860 Ile Glu Ala Ala Leu Ser Ala Leu Pro Gln Val Arg Glu Ala Val Val 865 870 875 880 Ile Leu Arg Asp Asp Pro Gly Arg Glu Lys Arg Leu Val Ala Tyr Val 885 890 895 Val Pro Arg Glu Gly Ala Val Gly Glu Gly Ala Ser Leu Asp Ala Ala 900 905 910 Asp Leu Ser Glu Arg Leu Gly Lys Val Leu Pro Val His Met Ile Pro 915 920 925 Ala Ala Tyr Val Val Leu Asp Ala Ile Pro Leu Asn Ser Asn Gly Lys 930 935 940 Thr Asp Arg His Ala Leu Pro Val Pro Gln Trp Thr Val Thr Glu Gly 945 950 955 960 Ile Ala Leu Pro Gly Thr Asp Ala Glu Lys Arg Leu Ala Ala Leu Trp 965 970 975 Cys Glu Ile Leu Gly Leu Glu Gln Ile Gly Ile His Asp Ser Phe Phe 980 985 990 Val Leu Gly Gly Asp Ser Leu Ala Ala Ala Gly Met Ile Ser Ala Val 995 1000 1005 Arg Ser Arg Leu Lys Gly Glu Ile Pro Leu Gly Ala Val Phe Glu 1010 1015 1020 Thr Pro Thr Ile Ala Ala Leu Ala Val His Leu Asp Glu Ala Ser 1025 1030 1035 Ser Gly Ser Pro Leu Ile Glu Pro Val Leu Ala Ile Arg Ala Lys 1040 1045 1050 Gly Glu Arg Pro Pro Leu Phe Cys Ile His Pro Val Leu Gly Leu 1055 1060 1065 Gly Trp Ser Phe Phe Ser Leu Ala Gln His Leu Ser Glu Asp Val 1070 1075 1080 Pro Val Tyr Ala Leu Gln Ser Asp Gly Leu His Asp Leu Ala Ala 1085 1090 1095 Leu Pro Arg Ser Ile Glu Asp Met Ala Ala Leu Tyr Val Gln Arg 1100 1105 1110 Ile Arg Lys Ile Gln Pro Gln Gly Pro Tyr His Leu Leu Gly Trp 1115 1120 1125 Ser Leu Gly Gly Leu Ile Ala His Glu Met Thr Arg Gln Leu Gln 1130 1135 1140 Ala Glu Gly Gln Ala Ile Ala Phe Leu Gly Met Met Asp Ser Tyr 1145 1150 1155 His Phe Lys Pro Ala Ala Gln Leu Gln Asp Asp Ala Thr Leu Ala 1160 1165 1170 Arg Ala Ala Leu Gly Phe Leu Gly Phe Asp Glu Lys Ala Ala Gly 1175 1180 1185 Asp Lys Pro Ser Leu Ala Glu Leu Gly Asp Phe Val Leu Lys Met 1190 1195 1200 Phe Asp Thr Asp Asn His Val Leu Leu Glu Gln Val His Gln Phe 1205 1210 1215 Asp Pro Glu Phe Val Glu Arg Ala Lys Ala Thr Ile Leu His Asn 1220 1225 1230 Leu Glu Ile Ala Gln Arg Phe Thr Pro Gly Lys Ile Asp Ala Asp 1235 1240 1245 Val His Phe Phe Arg Ala Glu Pro Ser Ala Gly Ser Asp Ala Leu 1250 1255 1260 Asn Thr Ile Leu Asn Tyr Asp Ala Glu Thr Trp Leu Pro His Val 1265 1270 1275 Glu Gly Arg Val Tyr Leu Arg Asp Met Thr Cys Asn His His Asp 1280 1285 1290 Met Leu Asn Ile Glu Pro Ala Ser His Ile Ser Ala Val Val Gln 1295 1300 1305 Ala Glu Leu Leu Arg Glu Phe Leu Val Leu Arg Arg Pro Gln Thr 1310 1315 1320 Glu Val Lys Ile Glu Arg Val Ser Arg Phe Ala 1325 1330 137524DNAA. vitis strain F2/5misc_feature(1)..(7524)avi4330 13ttgtttgatc ttttgcgtgc cggcgcatgc actgtctcga ccttgcccgg ggatcgttgg 60gatattgcgc gctactggca tcccgaaatc ggcacgcctg gcaaatatta caccttcgct 120gccggtgtga tggatggaat ctatcagttc gatcccgccc tgttcggcat gtcacggcgt 180gaagccgcct ttatggaccc gcagcagcgc atcctgctgg agttgacctg gcgggcgctg 240gaagatgcca atatccccgc caatagtctg tccggccaga atgtcgcggt ctatgtcggt 300gcctccagtt tcgaccatgc caatctggcg gcggaagatc cggctggtcc cggtccgcat 360ttcatgaccg gcaatacgct atcggtggtc tccaaccgta tctctcatgt cttcggcctg 420aatggtccga gcatgacggt cgatacggcc tgctcgtcct cgctggtcgc actcgatcac 480gcggtgcgcg cgattgcgtc tggcgaggtt gacaccgcca ttgttgccgg tgtcaacgtt 540ctggtccatc cgctgccttt cgttggcttt gcgcaggcgc gcatgctgtc gatcgacggt 600ttgtgcaagg cctatgccaa tgacggtatc gggtatgttc gggccgaagg cggtgccgtg 660ctggtgctgc gatctagcga taaggcccgg cgcgaaggcg accgcagcca cgcgactatt 720gttgccagcg gcaccaatgc agccgggcgc accaacggta tttcgctgcc gtcgcgcgag 780gcgcaggccg ttctgttgcg cgccgtctat gacgacaatg gtctcgaccc ggatcggctg 840gcctttatcg aaggccatgg caccggcacc aaggtcggcg atcccgccga agtctggtcg 900cttggcacgg tcatcggcca gcgtcgcaag gaacctgtgt ggatcggctc gatcaaaacc 960aatatcggtc ataccgaacc cgcatccggc ctgcttggcg tgatgaaggc gatgatggct 1020ttgcagcacg acctgcttcc ggcctcgctg catttcaatg agccgaatga cacgattgat 1080ttcgatggcc tgaatgtccg ggttgcctcg caggccgttg cactggcacg cgatggtgcg 1140ccgcgccttg ccggaatcaa ctcctttggc tttggcggcg ccaatgcaca tgtggtgatt 1200gccgatccgc agagacctga tttggtgcct ggtttggcgc aagacgcggc taatccagcc 1260ggtgccggtc gcttgttcat ggccagcgcc cactctcagg aaagcctgaa agcgttgctg 1320gacagttacg acaaggcctt tgcggccgct ggaagcgatg gtgatctgga agacctgatc 1380tcggcggccg cctccaaccg tgcgccgctc cggcaccgtt tcgttgccag tggcagcgcg 1440gatgccatcg tcaaggcagt gcaggaacgg cttgccacag cgaagaccgg cggcgaaacc 1500ggcgaagcct tgtcgcgcaa cggcaagctc gccttcgtgt tttctggcaa tggcgcccaa 1560tgggcgggca tgggtcttga tgcctaccgg gcaaacgccc gcttccgcga gagctatgag 1620cggatcgcca cgctctttac ggcgcattcg gaccttgacc tcgttgcggc cttgaccgat 1680ccggatctgg aagcccgatt gaaagatacc aggcttgccc agcctatgct gtttgccatc 1740caggcctcgc tgtcggatgc gctgcaactt gccggtctgg tgccggatgc tgtttacggt 1800cattcggtcg gtgaagtggc tgccgcctat gtatcgggtg cgctgtcgct gaaggatgcc 1860gtctgtgtca tcgccaaacg gtcgcagcat caggcggtgt tggccggtga aggcacgatg 1920gcggccctga agcttggcga ggccgatgcg cgcgccatgc tggccgagct tggctttgat 1980gacctgacga ttgccgcgat caatgcgccc aattcggtga ccgtgtctgg tcgtgaagac 2040gcgatccggg cgctgaggga acatgcccgc aagcagcggg tgccagccca ggtgctggac 2100attgactatc ccttccatca tccgctgatc gataaggcga aggctgcctt ttccgccgat 2160ccgccgctga ttaccccgcg ccagaccacc cttccattca tctccacggt gacgggtgag 2220caactggagg gcaccgcact gacttcggat tactggtgga agaatgtccg ccagccggtg 2280cagttccaag gggcgacgga agcggccatc gcgcttggtt gcaccgtgtt cgttgaaatt 2340tctccgcgtg cgatccttgg cggctatgtg tcggaaacgg cgcagcatgt gtcttcgacg 2400gttgccgtca gtgccagcct tagccgtgag gctccggatg agacggtcga tccggtggcg 2460cgggctttgg cgcgtgctgt cgccagcggc gccaaggtgg atgaggccaa ggtcttcggt 2520ccgcgccgcg ccgatatcgt cctgccgggc gtgcctttcg agcgggccga tctgcgaccg 2580gagccgacca gtgaccgcgt cgatctctat ggccggttcg gccagacggc ctatcggttg 2640agcggctggc gggtcgattt gaatggcggg cattggaaaa accatctcga cgcgcacctg 2700tttccggatc tggccgaaca tgtggtcgat ggccgggcca ttttgccggg cagcggtttt 2760gtggaaattg ccatttcggc ggcgcaggcg catttcggct ccgaccagct tgagatcagc 2820aatgtcgaga tcatgcgacc gctggaattg agcgacagcc ggatcgtcga actttccacg 2880ctgatttccg ccgcgaccgg cgatcttcag atccgctcgc gcgagcggct gagcgatgac 2940gactggacgg tgaatgcggt tgcccgggtt cgcaagctca cggcctccga actggacgat 3000gccgtggatt tcgacctgtc cagcccgaca tcagagctgg ataaatctgc cgcctatcgg 3060acggcgcgca atttcggcct ggattatggg ccgcgcttcc aattgctgga aaaggcgatc 3120tgccatggcg agcggcttgt cgaagtgttc ctgaagccgg cagccgcgcc gggtcacccg 3180ttgctgcgct ataatctcaa cccgatgtcg gttgatgcga tgttccacgg gttggtggcg 3240ctgtttggcc gcttcagcgg cgagcagggt ggtgcgcctt atattcccgt gcgtttcgga 3300cgtgtccgca catgggttct cggtagcccg gtccatcggg cggtgatcga gatcgaacgg 3360atcagcgaca gctcgatcaa ggccaatttt catctctatg gtgagacggg cgagcggatc 3420gccagcttga gcgatagccg gttccgacgc acctatctga agcagcacaa gacgctcgat 3480ggacttgcct atcattatga aaccatcgcg ctacccctgg tgaaagatca gggtgcggcc 3540ctggttgcac cgttgggtga tgttttcgca tgtcaggaac gtgagctgga caatgccacg 3600gtgctgatcc aggcctgcgt gctcagcgcc ttctatgcgc tggccgagtg ccttgccggt 3660caagatcggc gcgtgtcgct ggtcgatctg ccgggcgatg tgcggttgcg gcggttcctg 3720accaatgccc tgcacagtct gaccgatagc ggctttgccc agtatgcgga tggcagctgg 3780acgcttgaag atggcagcga tcttccgccc gctttcgagc tgatccggga actctatcgg 3840gatttcccgg aacgcaccgt tgaactggtg atgatcaatg acgtgctgac agccgttaat 3900gcagcgctca atcagcctgc

aggtgttgtg gacgagggtt tggattggga cggggtgatc 3960agcgaagcga cgctggacca tttcgccgtt cattccacgc tcgccaccga gagtcatagg 4020gtgttgttga aggctgtgat cgactgtctc tcgaccttgg atgccgatgc gccgccgctg 4080gtggtcgagc ttggcgccag ctcgcttcac cttagccgca agctcgccga tctggttcgt 4140gcggctggcg gcaatctggt gatttacgag cctgaggcag gcctgcgccg caatctggag 4200ctgtctttcg aggctgatcc gcgcgtcact gtcgtggacg caaagggatt ggaccagctt 4260tcgcttttga aagcgccggt cgatctaatt gccagcgccc atgccgatct ttgccgcttg 4320ctggatggtg aaatgctggc acggctcagc catggcgcct tggcctcggc ccgtcgcctt 4380gtcgccgtgc aaccagcgcc cggcctattg catgatttcg ccttcggtct gctggatggt 4440tggttcgaca ggacggtttc ggaggaattt ccgctcggcc gatttggcgg tagcgaagac 4500tggatgaaat ccctgcaaca ggccggtttt gcggcggccc atgcccggca attgcaggtg 4560gatggcggta gccttatcgt ggcggaagcc cagggtcggg ctgacgtggc cgagccatat 4620gatgcgacgc gtcaggacgg tgggtcggtg gatgaggttg tggccagcgg gccagtgatc 4680atcgttcatg aaaaaactgc ggatcttgcc cggctttcgg cggcagcaag agcgcttggt 4740ggttcccagg tgtctttgct gtgcctgtca ggcagctttg aaacggaccg gtcgatgctg 4800gccgatgcac tgggcaaggc tggaccagcc gtgggcggaa tggtctggct gatgccggat 4860accgatgcgg ctgcggatgg atcgctgctg ttgcaggaca gggtcggcgc tcttagcgcg 4920ctggccatgg cgtttggcga tgttgcggca tcaacgtctg atgctgcgag gacgcttccg 4980gtcactctgg ttctgcccgg cggtgcgccc gtcaccggct ttaccggcaa ggacttgacc 5040cgctccagtc atgcggggcc tgtcaatgca ggtctctggg cctttgcccg cgtattgcgc 5100aatgagttcg acctgttcga catgcaggtc gtcgataccg gaccctccag caatacgctg 5160gaaatcatgc tggactgggg catgcgcctg ctggccgcca agggcgacaa ccgcgaatgg 5220ctggtggagc cggaaaccgg gcggatggcc gagattcgtg ccgtgcctgg tccggccccg 5280ctgacggcgc agcgtaccgt tgcttttgag gcagcggtca ttcgccagca ggtgccgtcg 5340caggtcgcca gcatccgctg ggaaagctgc ccggtcccag tcatcggtcc aaccgaagtg 5400ctggtgaaga ccgcagcgac aggcttgaat ttccgcgacg tgatgtgggc catgggcctg 5460ctgccggaag aagcgcttga ggacggcttt gccggggcct ccatcggcat ggaatttgcc 5520ggtgaagtgg tcgctgttgg cggcaaggtg agcgatcttg ccctcgggga caaggtgatg 5580gcaatcgccg ctgcggcttt cggcacccat gtcaaggttg agcgggctgg ggtggcgaag 5640ctgccggatg gcgtggaccc ggtatcggcg gcgaccattc cggtggtctt cctgaccgcc 5700tattatgcca tccatgagct tgggcgggta cgtccgggcg aaaccatcct tattcacggt 5760gccgctggcg gcgtcgggct tgcggctttg caggtcgccc ggcatttcgg cgccaagatc 5820attgccacgg caggcaccgt cgaaaagcgg cgcttcctgg aaacgctggg ggcggaccat 5880gtgttcgata gccgctcgct cggttttgtc ggcgatgtgc ttgacgtgac cggcggcgaa 5940ggtgtcgatc tggtgttgaa ctcgctgttt ggcgaggcga tggaaaaatc cctgtcgctg 6000gttaagccgt tcgggcgttt ccttgagctt ggcaagcgcg attattacgc cgacagcaag 6060atcggcctgc ggccattccg gcgcaatgtc agttatttcg gcattgatgc cgaccaattg 6120ctggtcctgc atcccgatct gtcgcgccgt atgctggccg aaatcggtgg cttgttcgag 6180cagggcgtgt tcacgccatt gccgttccgc gccttcgaac acgatgagat tggtgatgcc 6240ttccgcctga tgcagaatgc cggccatatc ggcaagatcg ttgtcctgcc gccggttgcg 6300ggccgtgacc gcgtcgcggt aaagtctgcg cgccggatgg tggtcgatgc ggatggtatg 6360catctggttg tcggcggtat cggcggtttc ggccttgccg cagccgattg gctggtagag 6420cagggcgctc gccatatcgc cttatcgacg cggcgcgggc tggttgatgc ggagacgcag 6480accgtggttg accgctgggc caagcagggc gtgacggcct atattcgcgg ctgcgacgtg 6540accagtgaag cggccttgtc ggcgcttttg acggagcttc gcgccattgc gccgttgaag 6600accgttatcc atgcggcaat ggtgctggac gatgccttca tctccaattt gacgcgggcg 6660cgaaaccagc cggtgatcga cgtcaaggcc aagggtgctg tacttcttga ccggttgacc 6720cggcaggacg ggatcgacaa tttcattctg ttttcgtcga tcaccaccta tgtcggcaat 6780cccggtcagg gcaattatgt cgctgccaac ggcttcctgg aaggcttggc gcgggcgcgt 6840cgcgccgatg gtctggctgg tcttgccatc ggcttcgggg caatcggcga tgcgggttat 6900ctggcccgta atgcgcaggt taatgaacgg cttggacgcc ggatcggcaa gacggcgctg 6960gatgcccgcg atgcgctttc ggcggtcggg cgctatattg ctgccgatac cggctctgtc 7020gatgcagctg tggtgatgat ttccgaattc gactgggctg ctgcacattc gctttccgtg 7080gtcaacgaac cgctgttttc gttgatcatg cgccgcagca accagcatgc cggtggcagt 7140gagggtggcg agatcgatct ggtggcgctg attgatggca aggcgccggc tgcggcgcag 7200gacgtgctgt ttacggtgct tgccggtgaa attgccgata cgctgcgggt gcccaaggaa 7260agcatcgggc tgaacagcgt gctgaaggac atcgggctgg acagtttgat ggctgtcgaa 7320ctggggatga atttcgagca gaataccggc tttgacattc ccctcagcag ccttgccgac 7380aacgccactg tcggagacct gacgcgacgc ctctatgaaa aagtcagcct gcgcggacgg 7440acaggcgaca aggatgaagc gctacctgag gacagcaaga tcatggacga tctgcatcgt 7500cgccacagcg ggcaggacca gtaa 7524142507PRTA. vitis strain F2/5MISC_FEATURE(1)..(2187)avi4330 14Met Phe Asp Leu Leu Arg Ala Gly Ala Cys Thr Val Ser Thr Leu Pro 1 5 10 15 Gly Asp Arg Trp Asp Ile Ala Arg Tyr Trp His Pro Glu Ile Gly Thr 20 25 30 Pro Gly Lys Tyr Tyr Thr Phe Ala Ala Gly Val Met Asp Gly Ile Tyr 35 40 45 Gln Phe Asp Pro Ala Leu Phe Gly Met Ser Arg Arg Glu Ala Ala Phe 50 55 60 Met Asp Pro Gln Gln Arg Ile Leu Leu Glu Leu Thr Trp Arg Ala Leu 65 70 75 80 Glu Asp Ala Asn Ile Pro Ala Asn Ser Leu Ser Gly Gln Asn Val Ala 85 90 95 Val Tyr Val Gly Ala Ser Ser Phe Asp His Ala Asn Leu Ala Ala Glu 100 105 110 Asp Pro Ala Gly Pro Gly Pro His Phe Met Thr Gly Asn Thr Leu Ser 115 120 125 Val Val Ser Asn Arg Ile Ser His Val Phe Gly Leu Asn Gly Pro Ser 130 135 140 Met Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu Asp His 145 150 155 160 Ala Val Arg Ala Ile Ala Ser Gly Glu Val Asp Thr Ala Ile Val Ala 165 170 175 Gly Val Asn Val Leu Val His Pro Leu Pro Phe Val Gly Phe Ala Gln 180 185 190 Ala Arg Met Leu Ser Ile Asp Gly Leu Cys Lys Ala Tyr Ala Asn Asp 195 200 205 Gly Ile Gly Tyr Val Arg Ala Glu Gly Gly Ala Val Leu Val Leu Arg 210 215 220 Ser Ser Asp Lys Ala Arg Arg Glu Gly Asp Arg Ser His Ala Thr Ile 225 230 235 240 Val Ala Ser Gly Thr Asn Ala Ala Gly Arg Thr Asn Gly Ile Ser Leu 245 250 255 Pro Ser Arg Glu Ala Gln Ala Val Leu Leu Arg Ala Val Tyr Asp Asp 260 265 270 Asn Gly Leu Asp Pro Asp Arg Leu Ala Phe Ile Glu Gly His Gly Thr 275 280 285 Gly Thr Lys Val Gly Asp Pro Ala Glu Val Trp Ser Leu Gly Thr Val 290 295 300 Ile Gly Gln Arg Arg Lys Glu Pro Val Trp Ile Gly Ser Ile Lys Thr 305 310 315 320 Asn Ile Gly His Thr Glu Pro Ala Ser Gly Leu Leu Gly Val Met Lys 325 330 335 Ala Met Met Ala Leu Gln His Asp Leu Leu Pro Ala Ser Leu His Phe 340 345 350 Asn Glu Pro Asn Asp Thr Ile Asp Phe Asp Gly Leu Asn Val Arg Val 355 360 365 Ala Ser Gln Ala Val Ala Leu Ala Arg Asp Gly Ala Pro Arg Leu Ala 370 375 380 Gly Ile Asn Ser Phe Gly Phe Gly Gly Ala Asn Ala His Val Val Ile 385 390 395 400 Ala Asp Pro Gln Arg Pro Asp Leu Val Pro Gly Leu Ala Gln Asp Ala 405 410 415 Ala Asn Pro Ala Gly Ala Gly Arg Leu Phe Met Ala Ser Ala His Ser 420 425 430 Gln Glu Ser Leu Lys Ala Leu Leu Asp Ser Tyr Asp Lys Ala Phe Ala 435 440 445 Ala Ala Gly Ser Asp Gly Asp Leu Glu Asp Leu Ile Ser Ala Ala Ala 450 455 460 Ser Asn Arg Ala Pro Leu Arg His Arg Phe Val Ala Ser Gly Ser Ala 465 470 475 480 Asp Ala Ile Val Lys Ala Val Gln Glu Arg Leu Ala Thr Ala Lys Thr 485 490 495 Gly Gly Glu Thr Gly Glu Ala Leu Ser Arg Asn Gly Lys Leu Ala Phe 500 505 510 Val Phe Ser Gly Asn Gly Ala Gln Trp Ala Gly Met Gly Leu Asp Ala 515 520 525 Tyr Arg Ala Asn Ala Arg Phe Arg Glu Ser Tyr Glu Arg Ile Ala Thr 530 535 540 Leu Phe Thr Ala His Ser Asp Leu Asp Leu Val Ala Ala Leu Thr Asp 545 550 555 560 Pro Asp Leu Glu Ala Arg Leu Lys Asp Thr Arg Leu Ala Gln Pro Met 565 570 575 Leu Phe Ala Ile Gln Ala Ser Leu Ser Asp Ala Leu Gln Leu Ala Gly 580 585 590 Leu Val Pro Asp Ala Val Tyr Gly His Ser Val Gly Glu Val Ala Ala 595 600 605 Ala Tyr Val Ser Gly Ala Leu Ser Leu Lys Asp Ala Val Cys Val Ile 610 615 620 Ala Lys Arg Ser Gln His Gln Ala Val Leu Ala Gly Glu Gly Thr Met 625 630 635 640 Ala Ala Leu Lys Leu Gly Glu Ala Asp Ala Arg Ala Met Leu Ala Glu 645 650 655 Leu Gly Phe Asp Asp Leu Thr Ile Ala Ala Ile Asn Ala Pro Asn Ser 660 665 670 Val Thr Val Ser Gly Arg Glu Asp Ala Ile Arg Ala Leu Arg Glu His 675 680 685 Ala Arg Lys Gln Arg Val Pro Ala Gln Val Leu Asp Ile Asp Tyr Pro 690 695 700 Phe His His Pro Leu Ile Asp Lys Ala Lys Ala Ala Phe Ser Ala Asp 705 710 715 720 Pro Pro Leu Ile Thr Pro Arg Gln Thr Thr Leu Pro Phe Ile Ser Thr 725 730 735 Val Thr Gly Glu Gln Leu Glu Gly Thr Ala Leu Thr Ser Asp Tyr Trp 740 745 750 Trp Lys Asn Val Arg Gln Pro Val Gln Phe Gln Gly Ala Thr Glu Ala 755 760 765 Ala Ile Ala Leu Gly Cys Thr Val Phe Val Glu Ile Ser Pro Arg Ala 770 775 780 Ile Leu Gly Gly Tyr Val Ser Glu Thr Ala Gln His Val Ser Ser Thr 785 790 795 800 Val Ala Val Ser Ala Ser Leu Ser Arg Glu Ala Pro Asp Glu Thr Val 805 810 815 Asp Pro Val Ala Arg Ala Leu Ala Arg Ala Val Ala Ser Gly Ala Lys 820 825 830 Val Asp Glu Ala Lys Val Phe Gly Pro Arg Arg Ala Asp Ile Val Leu 835 840 845 Pro Gly Val Pro Phe Glu Arg Ala Asp Leu Arg Pro Glu Pro Thr Ser 850 855 860 Asp Arg Val Asp Leu Tyr Gly Arg Phe Gly Gln Thr Ala Tyr Arg Leu 865 870 875 880 Ser Gly Trp Arg Val Asp Leu Asn Gly Gly His Trp Lys Asn His Leu 885 890 895 Asp Ala His Leu Phe Pro Asp Leu Ala Glu His Val Val Asp Gly Arg 900 905 910 Ala Ile Leu Pro Gly Ser Gly Phe Val Glu Ile Ala Ile Ser Ala Ala 915 920 925 Gln Ala His Phe Gly Ser Asp Gln Leu Glu Ile Ser Asn Val Glu Ile 930 935 940 Met Arg Pro Leu Glu Leu Ser Asp Ser Arg Ile Val Glu Leu Ser Thr 945 950 955 960 Leu Ile Ser Ala Ala Thr Gly Asp Leu Gln Ile Arg Ser Arg Glu Arg 965 970 975 Leu Ser Asp Asp Asp Trp Thr Val Asn Ala Val Ala Arg Val Arg Lys 980 985 990 Leu Thr Ala Ser Glu Leu Asp Asp Ala Val Asp Phe Asp Leu Ser Ser 995 1000 1005 Pro Thr Ser Glu Leu Asp Lys Ser Ala Ala Tyr Arg Thr Ala Arg 1010 1015 1020 Asn Phe Gly Leu Asp Tyr Gly Pro Arg Phe Gln Leu Leu Glu Lys 1025 1030 1035 Ala Ile Cys His Gly Glu Arg Leu Val Glu Val Phe Leu Lys Pro 1040 1045 1050 Ala Ala Ala Pro Gly His Pro Leu Leu Arg Tyr Asn Leu Asn Pro 1055 1060 1065 Met Ser Val Asp Ala Met Phe His Gly Leu Val Ala Leu Phe Gly 1070 1075 1080 Arg Phe Ser Gly Glu Gln Gly Gly Ala Pro Tyr Ile Pro Val Arg 1085 1090 1095 Phe Gly Arg Val Arg Thr Trp Val Leu Gly Ser Pro Val His Arg 1100 1105 1110 Ala Val Ile Glu Ile Glu Arg Ile Ser Asp Ser Ser Ile Lys Ala 1115 1120 1125 Asn Phe His Leu Tyr Gly Glu Thr Gly Glu Arg Ile Ala Ser Leu 1130 1135 1140 Ser Asp Ser Arg Phe Arg Arg Thr Tyr Leu Lys Gln His Lys Thr 1145 1150 1155 Leu Asp Gly Leu Ala Tyr His Tyr Glu Thr Ile Ala Leu Pro Leu 1160 1165 1170 Val Lys Asp Gln Gly Ala Ala Leu Val Ala Pro Leu Gly Asp Val 1175 1180 1185 Phe Ala Cys Gln Glu Arg Glu Leu Asp Asn Ala Thr Val Leu Ile 1190 1195 1200 Gln Ala Cys Val Leu Ser Ala Phe Tyr Ala Leu Ala Glu Cys Leu 1205 1210 1215 Ala Gly Gln Asp Arg Arg Val Ser Leu Val Asp Leu Pro Gly Asp 1220 1225 1230 Val Arg Leu Arg Arg Phe Leu Thr Asn Ala Leu His Ser Leu Thr 1235 1240 1245 Asp Ser Gly Phe Ala Gln Tyr Ala Asp Gly Ser Trp Thr Leu Glu 1250 1255 1260 Asp Gly Ser Asp Leu Pro Pro Ala Phe Glu Leu Ile Arg Glu Leu 1265 1270 1275 Tyr Arg Asp Phe Pro Glu Arg Thr Val Glu Leu Val Met Ile Asn 1280 1285 1290 Asp Val Leu Thr Ala Val Asn Ala Ala Leu Asn Gln Pro Ala Gly 1295 1300 1305 Val Val Asp Glu Gly Leu Asp Trp Asp Gly Val Ile Ser Glu Ala 1310 1315 1320 Thr Leu Asp His Phe Ala Val His Ser Thr Leu Ala Thr Glu Ser 1325 1330 1335 His Arg Val Leu Leu Lys Ala Val Ile Asp Cys Leu Ser Thr Leu 1340 1345 1350 Asp Ala Asp Ala Pro Pro Leu Val Val Glu Leu Gly Ala Ser Ser 1355 1360 1365 Leu His Leu Ser Arg Lys Leu Ala Asp Leu Val Arg Ala Ala Gly 1370 1375 1380 Gly Asn Leu Val Ile Tyr Glu Pro Glu Ala Gly Leu Arg Arg Asn 1385 1390 1395 Leu Glu Leu Ser Phe Glu Ala Asp Pro Arg Val Thr Val Val Asp 1400 1405 1410 Ala Lys Gly Leu Asp Gln Leu Ser Leu Leu Lys Ala Pro Val Asp 1415 1420 1425 Leu Ile Ala Ser Ala His Ala Asp Leu Cys Arg Leu Leu Asp Gly 1430 1435 1440 Glu Met Leu Ala Arg Leu Ser His Gly Ala Leu Ala Ser Ala Arg 1445 1450 1455 Arg Leu Val Ala Val Gln Pro Ala Pro Gly Leu Leu His Asp Phe 1460 1465 1470 Ala Phe Gly Leu Leu Asp Gly Trp Phe Asp Arg Thr Val Ser Glu 1475 1480 1485 Glu Phe Pro Leu Gly Arg Phe Gly Gly Ser Glu Asp Trp Met Lys 1490 1495 1500 Ser Leu Gln Gln Ala Gly Phe Ala Ala Ala His Ala Arg Gln Leu 1505 1510 1515 Gln Val Asp Gly Gly Ser Leu Ile Val Ala Glu Ala Gln Gly Arg 1520 1525 1530 Ala Asp Val Ala Glu Pro Tyr Asp Ala Thr Arg Gln Asp Gly Gly 1535 1540 1545 Ser Val Asp Glu Val Val Ala Ser Gly Pro Val Ile Ile Val His 1550 1555 1560 Glu Lys Thr Ala Asp Leu Ala Arg Leu Ser Ala Ala Ala Arg Ala 1565 1570 1575 Leu Gly Gly Ser Gln Val Ser Leu Leu Cys Leu Ser Gly Ser Phe 1580 1585 1590 Glu Thr Asp Arg Ser Met Leu Ala Asp Ala Leu Gly Lys Ala Gly 1595 1600 1605 Pro Ala Val Gly Gly Met Val Trp Leu Met Pro Asp Thr Asp Ala 1610 1615 1620 Ala Ala Asp Gly Ser Leu Leu Leu Gln Asp Arg Val Gly Ala Leu 1625 1630 1635 Ser Ala Leu Ala Met Ala Phe Gly Asp Val Ala Ala Ser Thr Ser 1640 1645 1650 Asp Ala Ala Arg Thr Leu Pro Val Thr Leu Val Leu Pro Gly Gly 1655 1660 1665 Ala Pro Val Thr Gly Phe Thr Gly Lys Asp Leu Thr Arg Ser Ser 1670 1675 1680 His Ala Gly Pro Val Asn Ala Gly Leu Trp Ala Phe Ala Arg Val 1685 1690 1695 Leu Arg Asn Glu Phe Asp Leu Phe Asp Met Gln Val

Val Asp Thr 1700 1705 1710 Gly Pro Ser Ser Asn Thr Leu Glu Ile Met Leu Asp Trp Gly Met 1715 1720 1725 Arg Leu Leu Ala Ala Lys Gly Asp Asn Arg Glu Trp Leu Val Glu 1730 1735 1740 Pro Glu Thr Gly Arg Met Ala Glu Ile Arg Ala Val Pro Gly Pro 1745 1750 1755 Ala Pro Leu Thr Ala Gln Arg Thr Val Ala Phe Glu Ala Ala Val 1760 1765 1770 Ile Arg Gln Gln Val Pro Ser Gln Val Ala Ser Ile Arg Trp Glu 1775 1780 1785 Ser Cys Pro Val Pro Val Ile Gly Pro Thr Glu Val Leu Val Lys 1790 1795 1800 Thr Ala Ala Thr Gly Leu Asn Phe Arg Asp Val Met Trp Ala Met 1805 1810 1815 Gly Leu Leu Pro Glu Glu Ala Leu Glu Asp Gly Phe Ala Gly Ala 1820 1825 1830 Ser Ile Gly Met Glu Phe Ala Gly Glu Val Val Ala Val Gly Gly 1835 1840 1845 Lys Val Ser Asp Leu Ala Leu Gly Asp Lys Val Met Ala Ile Ala 1850 1855 1860 Ala Ala Ala Phe Gly Thr His Val Lys Val Glu Arg Ala Gly Val 1865 1870 1875 Ala Lys Leu Pro Asp Gly Val Asp Pro Val Ser Ala Ala Thr Ile 1880 1885 1890 Pro Val Val Phe Leu Thr Ala Tyr Tyr Ala Ile His Glu Leu Gly 1895 1900 1905 Arg Val Arg Pro Gly Glu Thr Ile Leu Ile His Gly Ala Ala Gly 1910 1915 1920 Gly Val Gly Leu Ala Ala Leu Gln Val Ala Arg His Phe Gly Ala 1925 1930 1935 Lys Ile Ile Ala Thr Ala Gly Thr Val Glu Lys Arg Arg Phe Leu 1940 1945 1950 Glu Thr Leu Gly Ala Asp His Val Phe Asp Ser Arg Ser Leu Gly 1955 1960 1965 Phe Val Gly Asp Val Leu Asp Val Thr Gly Gly Glu Gly Val Asp 1970 1975 1980 Leu Val Leu Asn Ser Leu Phe Gly Glu Ala Met Glu Lys Ser Leu 1985 1990 1995 Ser Leu Val Lys Pro Phe Gly Arg Phe Leu Glu Leu Gly Lys Arg 2000 2005 2010 Asp Tyr Tyr Ala Asp Ser Lys Ile Gly Leu Arg Pro Phe Arg Arg 2015 2020 2025 Asn Val Ser Tyr Phe Gly Ile Asp Ala Asp Gln Leu Leu Val Leu 2030 2035 2040 His Pro Asp Leu Ser Arg Arg Met Leu Ala Glu Ile Gly Gly Leu 2045 2050 2055 Phe Glu Gln Gly Val Phe Thr Pro Leu Pro Phe Arg Ala Phe Glu 2060 2065 2070 His Asp Glu Ile Gly Asp Ala Phe Arg Leu Met Gln Asn Ala Gly 2075 2080 2085 His Ile Gly Lys Ile Val Val Leu Pro Pro Val Ala Gly Arg Asp 2090 2095 2100 Arg Val Ala Val Lys Ser Ala Arg Arg Met Val Val Asp Ala Asp 2105 2110 2115 Gly Met His Leu Val Val Gly Gly Ile Gly Gly Phe Gly Leu Ala 2120 2125 2130 Ala Ala Asp Trp Leu Val Glu Gln Gly Ala Arg His Ile Ala Leu 2135 2140 2145 Ser Thr Arg Arg Gly Leu Val Asp Ala Glu Thr Gln Thr Val Val 2150 2155 2160 Asp Arg Trp Ala Lys Gln Gly Val Thr Ala Tyr Ile Arg Gly Cys 2165 2170 2175 Asp Val Thr Ser Glu Ala Ala Leu Ser Ala Leu Leu Thr Glu Leu 2180 2185 2190 Arg Ala Ile Ala Pro Leu Lys Thr Val Ile His Ala Ala Met Val 2195 2200 2205 Leu Asp Asp Ala Phe Ile Ser Asn Leu Thr Arg Ala Arg Asn Gln 2210 2215 2220 Pro Val Ile Asp Val Lys Ala Lys Gly Ala Val Leu Leu Asp Arg 2225 2230 2235 Leu Thr Arg Gln Asp Gly Ile Asp Asn Phe Ile Leu Phe Ser Ser 2240 2245 2250 Ile Thr Thr Tyr Val Gly Asn Pro Gly Gln Gly Asn Tyr Val Ala 2255 2260 2265 Ala Asn Gly Phe Leu Glu Gly Leu Ala Arg Ala Arg Arg Ala Asp 2270 2275 2280 Gly Leu Ala Gly Leu Ala Ile Gly Phe Gly Ala Ile Gly Asp Ala 2285 2290 2295 Gly Tyr Leu Ala Arg Asn Ala Gln Val Asn Glu Arg Leu Gly Arg 2300 2305 2310 Arg Ile Gly Lys Thr Ala Leu Asp Ala Arg Asp Ala Leu Ser Ala 2315 2320 2325 Val Gly Arg Tyr Ile Ala Ala Asp Thr Gly Ser Val Asp Ala Ala 2330 2335 2340 Val Val Met Ile Ser Glu Phe Asp Trp Ala Ala Ala His Ser Leu 2345 2350 2355 Ser Val Val Asn Glu Pro Leu Phe Ser Leu Ile Met Arg Arg Ser 2360 2365 2370 Asn Gln His Ala Gly Gly Ser Glu Gly Gly Glu Ile Asp Leu Val 2375 2380 2385 Ala Leu Ile Asp Gly Lys Ala Pro Ala Ala Ala Gln Asp Val Leu 2390 2395 2400 Phe Thr Val Leu Ala Gly Glu Ile Ala Asp Thr Leu Arg Val Pro 2405 2410 2415 Lys Glu Ser Ile Gly Leu Asn Ser Val Leu Lys Asp Ile Gly Leu 2420 2425 2430 Asp Ser Leu Met Ala Val Glu Leu Gly Met Asn Phe Glu Gln Asn 2435 2440 2445 Thr Gly Phe Asp Ile Pro Leu Ser Ser Leu Ala Asp Asn Ala Thr 2450 2455 2460 Val Gly Asp Leu Thr Arg Arg Leu Tyr Glu Lys Val Ser Leu Arg 2465 2470 2475 Gly Arg Thr Gly Asp Lys Asp Glu Ala Leu Pro Glu Asp Ser Lys 2480 2485 2490 Ile Met Asp Asp Leu His Arg Arg His Ser Gly Gln Asp Gln 2495 2500 2505 1511899DNAAvs locus 15gtcgccatga cccagcgata aatcgtggaa tggtcaaccg cgacgcccct ttcagccatc 60atctgttcaa gatcgcggta gctcacccca tagcggcaat accagcgaac tgcccacaag 120atcacctcgc cctgaaaatg acgccacttg aaatcgctca tcaaaagtct cgctgaaaac 180tatctccctt atttccagca caaacggaat ctttgcgaca gagccgtatt tccggccttt 240gatggttacc accatctcat cgaggtgcca tttatcggca aagccgccct ttgagcgccg 300gcgaagcaac gcagcaaact cggagccaaa tttggcggcc cattccgata ctgtctggaa 360cgaaacgtca ataccgcgtt cggccagcaa atcctcgaca tgccgcaggc tcaatggaaa 420ccggaagtac agccaaaccg cgtgagcgat aatctcggct ggaaagcggt gacgtttgta 480acgataggag gcgaaggtag acgaagaaat gttcatgccc actgctctat cgtacaaaac 540ttaaggcgaa actgccgctc agggcgcaag gcttatacta tagcctgact tgtattcgat 600tggcagaaaa aggcgatgat attccctgaa tgtctcgtct ggatcgagat cggcgaacaa 660atccggatga agccattttg cgatttgttg tatggcgatg aattcataag ggctattgta 720gaactgatgc catatgccat ggaaactgcg gtttttgacc gctttggtgc caatataggc 780atttcttgtc gtgaaccatt ccagttttcg ttcggcttcc tgtctgttgg cacccggccc 840caggggtatc cagtgtccac ccggaacata agcttcccaa ttggcgctcg tgaccacaac 900ctgatccggg tcggcagcaa tcacctgttc cgggttcaat tgtccgaagg ttccgggaag 960aaaatcaccg ccgatattgt gcccgccggc gatttcgaca tagcgaccaa aattctcatt 1020gccaaaggtg aggcagcaat catccgcata accgccaatc cgctcgacaa agacattcgg 1080tcgtgcgggc tttttttgtt caatcacatc cgtcacttgc gacaatgcct ttttcctgaa 1140ggcaataatt tcctcggcgc gcttttcacg tcccatgatc tcgcccaaaa ggcggattgt 1200tggttcggta ttgtgaaggg gttgatgccg gaaatcgata taaagaaccg gtattcccag 1260tgatgccagc ttttcgatat attcggactc tttgctggca cgctgggttt cgagattcaa 1320aagcacaaca tcaggctttt gaacaatggc agactcaaga tcaatcagac ctgcttcctt 1380gccgccaaag gttggcagtt gggccagttt cggaaacttc gcgacatatt gggcataggt 1440tgcggggtcg gcttcaatga ggtcattttt ccatcctgcc agaaacgcca gaggattctc 1500ggactccaat gacgcgacaa gatagagctg ccgcccttcg cccaacaaca tgcgatggac 1560aggaacaggc aatgacacct gccgaccagc aatgtctgtc acggtgatgc gatgcggcgt 1620tgttgctgca ttggatgttt gagccgctgg ctcctgtgcc acagcagatg gcatctgcgc 1680gacaagacac gacagcagag ccgcaaagag agtggtggct ggttttatca ccgttttatc 1740ctatgcggtt gggtaagggg ccgcttttga gcaagagcga caggccgata aaaaatgaca 1800ggatgtaaca cgccgatacc agggaaaatg cggtatcagc accccaaagc tgcatggacc 1860caacaccggc tgctataccc agaatggaag cgatttgctg ccagctttgc gcacggccca 1920aaatttcacc ttgccgattg aaggctgtgg actgggaaag acgcgaggta agaaccggtg 1980tcgtaccacc aagcagaatg ccccagacaa agtataatcc tgcaaaaacg ggaacgaggg 2040ttgtgtagcc cgctatcaaa gcgatgcttg cgcaggccag agcgatcgcg gtgtttgccg 2100ccaacacaaa gccaaggctg cgcattttga acagacgcgc ccagaggggg gcgccaatca 2160caaaaccaaa cgccatgagc ccgtagctga ggccaataat ccagtggttg gctccgaaag 2220cttgggtcat gtagagcgaa aacggcactt gcagcaccat tcggctggcc aacaatacgc 2280aaatcaaaac cagcaacccg acgactggtg cagaactttc tggatggccc ggggtgagcg 2340cgcttgaagc ggagcgcggt gcattctgtc tgatgggcgg gatgggcaat gttgcccagg 2400caacaagggc acatagacca catacggccc cggccgtgat attaacggcc gcgaatggca 2460ttgcatccag aatgagacca cccgcaaatg cgccgcccag cgaaccgaca ttggtcgcca 2520cctgcaacca ggcgaacaga ctggcgcgat cacgcccgct attgacctgc acggcatagg 2580cttgcgcggg cgcaatatag ccggcgaaag ccccctgtaa gaaccgcaga aacaggatga 2640cccagacatc ttgggcaagc gcaatcagaa gctgggtgat cgccaacccc gccaaggccc 2700gaaccatcat caagcgattg ccatagcggt cacccatacg cccccagaat gcgctggtga 2760gggaaatgcc aagcatgggg caaatataaa ccccgatact tgccaatccg aacgccgtat 2820ccgacgggct gagtaccctg atctgcagcg gccagaaagg accgctcatt tccatggctc 2880ccatggaaat gaactggagc gcaaacagca gcagaaacac aggcgtgctt gccctgagca 2940tggcaaaagc gttcagcatt cacggccaac cagaggattg ggcagctcat gctccacccg 3000atagtcgcta tatcgctgga gatgcatggt gagaaccgac cgggtgggcc attttttttc 3060aagcagggca tctcgttctt cttgccagaa cgtgtccgaa gacacacgag gccgcaatgt 3120ttcaaacgcc tcttgcgtta cctctttgat cactgtccag agcctatcat tgccgatact 3180atagtgctgg gtcaggcaca gagcaatttc atgcagatgg catataaagc aggcgtcgat 3240caaaaacgag cgaaccagcc caatgtcatc gtcaaaggtg gtgggcaaaa tcccagcacg 3300ctgaaagggt ttgaggtgat agccacgctg tttgagcaga ggcgcaaagc tgcggccatc 3360accaaaatcc cgaatgatca gcttttccgc agaaccggaa ggatcaaaca cgatggtggt 3420gttttgctga tgggcctcaa aagcgatgcc ataaagaaga tacatcgcca acgcaggccg 3480aaccaccacc tcaacatacc ggcggaaaaa gccgacaacg cttgcctgtt cctcattgcc 3540gtaacgggca atcagctcgc agatcaatgg tcgtccatcc ttgggacttg cacttagaag 3600tgcggcgacc gtcaccgcat agcgtccatc ctcgcgcttt aaaggctggg cgttgcgata 3660gaccaccgac aaaaatcgcc cgcgatgctc atcgccggta tcgggatcgt gcagaatggc 3720tcccaattct tcggtgaaga tttccaattg tgcggccatg tccggttcat tggccagaat 3780atcggtgatc aacgtggaaa gccgaggccc catgtgaatg gatttggcct gcaagctgcg 3840ctgctcgctg gtgagccaga tggcaaccgg caacttgatg aaagggcgca acgtttcatc 3900gtccggtaac atggtccgaa atgacatgga tggcgaggtg tcaatgtctg gcccgtccaa 3960cagcaagatg ccagaggcga tttcctgtcc aaattcgcgg cggacaaaat gctcgaggtg 4020ccatccgtgg atgggcaaag gaatccagtc ttgcgctgat ttgccctgtg cgctcagatg 4080gtctttccac gcggcaaaca attcgggaaa ctgggcagaa aaccagtcat tgtaatcagt 4140cacatgtggc attttttcca cataggccca accccgacgc aaggccgcaa tccggaccgg 4200caccttggcc ccgaattcag gcgaaagggc aacaacctct tcgggctgca aactcggttt 4260tgccttccat gtcggataaa acggatgacc ttcgagcgca ccccactggt caatcagcat 4320tgccgccaga tgtgcaggca aatgaccgta gagatagccc agaaaatcca ccgcaccgga 4380ttgtttgatc ttctcattca ggctcgcagc ccagacctgc cggtgtcggc gggccagcat 4440atcattgttg atgctgtcgg cgatatcgcg catcaaaacc tcaagcccat caggggccgg 4500agagatggac aacgaagcga acacttcccg cagcaaagca tcgggatggt caatccgggc 4560atggccgccg tcggcgtcga gaatttcaat ctcgccacgg ttttgcaagg tgccagcggg 4620tgccgcatgc aaatgtttaa aatgcagcac acggcgcgtg ttccacagcg gcaaccaggc 4680ttgatgcttg tcacgcgacc agagcaacgc ttcaggagcc agaagacgtt ctgcaaacag 4740gcagcgaaca aggcgactga ttgcattggc aacggcaaaa tcccgcaacg gctggtcatc 4800ggtctgcgca tggatgtcaa acaaaggcga aagcaaatgg gtcatcaggc agcctccagc 4860caatcatcag gaaccgggct taatccatcc ggcaaagcac catgatagtg gcgcgcccac 4920gtgtaggttt tcgagatggt tggaatcggt accgccagcc gctctgcgat cttgaccagc 4980aatgcctgcc cgcatgcaac atcctcatga aaggcacggc tttgcagatc gatcaaccag 5040ccttgaccat gatgattggg gatcagcggc aattgaattc ccgcataggc gctgtttgtt 5100ctgagcaggg aatacatcgt ttgactatcg gcaatctgat cgccataggc ttcaatcagt 5160tcctgccgca aaggtttgac ggagctgaga tcaagaccgg ttcgggtcgc aatcgcctga 5220catatggcct gattttcggc atcgaacatt tcgagcaagc gcgcgccttc ctcagggcaa 5280tcactccacc agcacagcgg ctcggagaac ggtttacgct cccagggagc gcccggcccc 5340agcaggccgt agagcaccgc tggatgcatc agcgcattgc cgggtgtcag ggtgatttcc 5400agatagtcct gcaaaagggt tacaggcgca ttgtaaagtg cggtcagcat tgtttgcaga 5460gccttggcac tttgggcgga ttcccgccga tgaaggccag caaacaaatg ggctttagcg 5520ccgcccatgc gaacgctctg tccggcaaca aggtcgtagg cgatatgcgg cacatccttc 5580aaaccccaga tgaccacatt atcccttgcg cctaagtgat cggcagccag ccagtcaaaa 5640ccgcaaaagc ccggaatagc acccacgaaa acctgcttgg tgctggagat actgcctgcg 5700atccgctgca aaacagcgga tctggcatgg gctggctggg taatgacaat cagatcagcg 5760ttcccaaccg ctttatcggg gttgttgcca acgtaatcag gacgcccgga cataccctga 5820ccatccggca aaaaagcctg ccatggagca ggatttccag cccaattggc ggcaagagtg 5880tcattctccg tcaaaacgga gagatggacc tgcggatttt gcttaaaaag cacggcattc 5940aaatgcccgg tgcggcctgc gccgcaaatg gcgaccctca tgccgcacct gccaagccat 6000tgaaccaacc atccaattga gctgcgattg ccggattgag aagcccgcgc ttttccatcc 6060actcatcgtc aaacacggat gccaggtatt tttcaccccc atctgcgacg gctgtaacaa 6120ttgtgccgct caatttaccg gatgcgatga attcgagagc tttgtaaatt gtacccccgg 6180ttgatccacc aacgagaata cctttgcgtc gggcaatata gcgtgccgtt tcaaatgctt 6240gcgtatcggt gacctgaaca ccttcatcaa tgcagctata atccagaacc ttgccgactt 6300catcgcctgc gggcgtacct gtgccagatt ggtaatagga atgccccggc ttaccaaaaa 6360caatggaacc agcaggctca accgcaattg tcttgatggc gggattgagg cgcttgaggc 6420gctgggatat gcccgtcatc gacccacccg tacccacgca accaacaaag gcatccaatc 6480catcaggcag ttgtgccatc aactcatcca caaatccggc atagccgtca ggattggcgg 6540gattgtcgga ctggttcatg aacagtgcac caggcagttg cgcgccgagc tgggcggcca 6600gtctctgcct ttcaacgacg gccacttcat cttcgcgata gtcgccttca acatagcgaa 6660tttcggcacc caaggcccgc atcatccgga ttttatccgg cgcggcgtga tgatccacga 6720ccgcgataaa gcgaagacca aactccagcg ccgcaagagc cagacctgtg cccgtgttgc 6780ctgatgagga ttcaacgatc gtgccgccac gcggcaggcg cccatccgcc aaggccgcaa 6840ccaccatgct gcgcgccatg cggtctttca ttgagccgcc aggattgttc ttctccattt 6900tgagcaaaag ttttgcattg cttgttgcca gatcaagcgc gatcaggggg gtctggccaa 6960tcaattgcgt gacggttgta tagagcatag tacctcctca caggtatccg gaataagttc 7020ggacagtcca tctgcggtcc tcctgacaca taagcggatt ggcatggggt ggcggtgaaa 7080ctggttttcc atcagatcca tctgatagcc gccggtattg acatagatga gcaaatcacc 7140agcttgcggg gtaatcggaa agtcgagcca gcggttggag acaatatctt catcaaggca 7200gctatgtccg gcaagatagg cgcgcacagg agtggtgcgt gcagcttccg gcccgtgcgg 7260caccacaatg ggatcgatca gaaattctga tgcgaaccat gtttcgcatg cactgaagct 7320gctgccctcc acaaaaattg ctgctgactg cgcacccagt gccttcactc tcgtcactct 7380gaaagccgtg attgcagtct gatccgcaag ggcccgcccc ggctccatcc ccagcgtaag 7440attttcctga cggaaatatg ttgcgacatc gcgcccgtcc tgcattttgg attgcaacag 7500gtgagttaac cactccgttg cgcaaacagg tcctccatag ggataaaaag actgtggcag 7560atggttggtg cgataatcct gcgccgtttg cgcctccaga aaggcggcgt aggtcgggtg 7620atcgacatat tgcaccggca acccaccacc aatatcgatc atgcggggag aaagccccat 7680gccgcgcgct ttggcaatga gatccgctgc ttcattcaat gcttcgatgc gcgccttaac 7740gctataacca ctcaagtgga aatggattcc atcaaatcgt agggctgggg tgcctgccaa 7800acgatgcagg cacgaaacaa cgtcttgagc atccatgccg aagcggcttt gcccttgatt 7860ttggggcctt aatctcaaaa gcacgggctg agggtcaccg gcgcttggca attccttcaa 7920aatatcatcg aattcctcaa cggaatcgat ggaaatcagg gccttgcagg ccatcaacgc 7980tctatgaaat gtcgatgttt tggcaggacc ggtcgcaaca atatcggcac cctttgcacc 8040caatgccaag gcatcctgca actcgtacag gctggagaca tcaacgcctc cttcagcctg 8100aagggcagct cccataagat tcacagactt gttgaccttc acaccataat aaattctgca 8160tggaacttga ctgccgctca atatggcctg caaagccaga agattatcgg ccagcagatc 8220aggccagatc aaatggagcg gcgagccaaa tctgttgaat ccttcccgca actgttcatg 8280cttgttttca ataaaatctg cgacctcgtc atgaatcagg ggcgtcagag tgtgagattg 8340cttggagcca agtctcatga cagcaccgcc ctgccgcctt gcatcgccga atccatggta 8400gccacaattc cggctaatac cgcgagcggt acatcacata cagggtaatc atccgcgaag 8460acgtgacgtg tcgttttgat cggaaatttt ttgccggacc gcgccatggc aaaaatatcg 8520tctggtgtaa tgcattcctc aggccgccaa ctcgtgacag acacacgctc ataatccagg 8580aggacaacag gaatgacagc gagattgagc tgccttgcga cctcaagccg gtgatgccca 8640tccatcacca gaaactgagt gcgctcgacc gcaatagggc gtgtccagat ctgctgacgt 8700aaaatggttt ggcgcaaatt ctcaacaacg ctttgatcga cttcttcagt gtcgacaagt 8760tttttgggag aggcaaaatg atatggcata aaatacagcc cttcggtgaa aatcgctatt 8820tccgaaaata tgactacata aatcatgttt aacatgtagc gtttatttcc agatgtcaat 8880ttacaatcct aaaaaaatat ccagtataaa cagatggata aaagatatct ggctatttca 8940ttcgcgatga aaataaaatc ttgactctta atgtcatgtt caatacccag ctgcttgggc 9000gtgtatttta aagagcatag ttcacggcaa ccttgccggt tctgctgagg gggatgctga 9060catgatgggt ttgaaaagtg gcgttgccat gggtgcaatt ctggctgctc tcggcactgt 9120tgcgcaggca gaagaggaca cggttctcaa gccaattgtt gttgagggac aatcctcatc 9180gcccaacgca accataggca aacagagcga accttatgca gggggcatgg tcaccggtag 9240cgctcgattg ggcagtctgg gcaaccgcag tttcatggat atgcctttca gcaccagcgg 9300atatactgca aaagtcatag aggataaggg agcatcaacg gttggtgatg tgatggagag 9360cgatgcatcg gtgcgcaata cgcatccgtc tggcggcatt gtcgactctt tctatatccg 9420tggctttccc atcggtgacg gcaattttgg tgaaattgcg tttgatggca tgttcggcgt 9480cgcaccaaac tatcgcgttt ttaccgatta tgcggaatcc gtagaagtcc tgaaagggcc

9540aacctccttc ctctatggca tttctcccaa tggtggtgtg ggcggaacca tcaatattgt 9600tccaaagcgt gctctggata cggacctgac ccgtgtcacc accagctatg aatcggatct 9660tcaggttgga acgcatgtgg atatcagccg ccgctatggc agtgaacggc aatttggcgt 9720ccggctgaat ggcagcgttc aaggtggtga tggcactatc gacgatcttt ctcgttttgc 9780ctatgtcggt gcggttgcgc tggactacga aggagaaaat ctgcgcgcaa cgctcgatgt 9840catcaatcaa tatgaacatt atgacgcgcc acagcgtcca ttctatccca cagccggcat 9900taaactgccg agtgcaccgg acaatcgtct gaatgtgcag gagtcctggg aatggtcagc 9960aacccgggag ttttcaacac tggggcgcgt tgaatatgac ctcagcgatg acgtgaccgt 10020gtttggtgcc gtcggtggtg ggaagtcaaa cgtcgagcgt ctgtttggca cgcccacaat 10080cacggactct gccggcaatg tcagcatcgt accgcagcat tatatttttg atgtgcagag 10140acgaaccgca gaaatcggca ctcgcggaca gttcgacacc ggcatcatcg agcactccgt 10200gacattgcag gccaattata tgctgcaatg gctgtcgcga ggctccaatt ccggtacggc 10260ccaaaccacc aatctgtata atccggttga tcgtgaggag caatttgttg ccaagccgtc 10320cagcgtaccg aaagtaacgg aaagcgaatt ctacggtgtg gctttatccg atacaatgtc 10380catatgggat gagcgggcgc aattgatggt tggcgggcgc tttcaacaca tcgattccga 10440aaactacagc tcgacaactg gcgctgtaac atcctcttcc gacgcgagcg caataacacc 10500catggttggt gtcgtggtta agccgtggga aaatgtatcg ctatatgcaa actatgcgga 10560agggttgagc atcggggaaa cggctccgac aaacgccgta aacgcaggcg aaaccctcag 10620cccctataaa tccaagcaat atgaagtggg aaccaagata gacaccggtc cggtgacgct 10680caccgccagt cttttccaga tcgaaaagcc gtttggtgtt ttggagacgc gtggcagcga 10740tctggttttc gaacgcggtg gcgaacagcg taaccggggt cttgaacttt cagcgttcgg 10800cgagttgacc gacaccgtga gactgcttgg cggcgttacg ttcatgcggg gcgagttgac 10860gaaaaccaac aatgcctcga cgcaaggcaa tgaccccatt ggcgtgccaa aagtcctcgt 10920aaacctcggt gcagaatggg acaccccatt cctgagcggc ttcaccctga cgggcaacgt 10980catccacacc ggcaaacaat atgccgatac agccaatatc caaaaactac cggcatggac 11040acgtcttgat ctcggcgcgc gctacaagac aacgatcaag gagcgacccg ttaccttccg 11100tgccgaagtc gaaaacgtct tcaacaagaa ttactggtca ggtgtcgcaa gctttggcac 11160tgtgacacaa ggggccccgt tgaccgttaa aatatcgatg acaaccgatt tttaagtctc 11220gttaaccact gatgtatcgt ttgcgagtca gcgcctttcg aagaggcgtt gtcttcgtct 11280atggggcttg actgtcgatt tccacttggg ccggcactgt aaacttaccg ccttgaacgc 11340aacatctggg cgtgaggaat tagttcatgc ggcgtgaagg cgcgcgattt ggtgccatgc 11400ttgcatggca gctgttcgca gttcgcgatg gtggacggat ggaatatcgt ggcggggaat 11460gtgaaaaagg ttggtgatcg ggtcatggat ggaaacgaaa cgctgaagat gtcgtgttga 11520cttgaagcgc ttcatgatcc tctcccgtcg tcggacgggc tgatgagagt tttccgaccg 11580atttttcaat cctttgtgag aacgatgctc aatgccgggc atgacctccc gctttgctgc 11640accataggat cgtagtttgt cggtaatcat cacacgcagc gcacggcctt gggctttcag 11700gagcttgcgc atcaaacgtt ttgccgcctt ggtattgctg cggttttgca ccagcacatc 11760gagaacaaag ccatcctgat caacggcgcg ccaaagccag tgtttccttc caccgatggt 11820gatgacaacc tcatcgagat gccatttgtc cccgagcctg ccggtagatc gcttgcggat 11880atcgttggca aaatgtcta 1189916471DNAA. vitis strain F2/5gene(1)..(471)P1388 16ttgtacgata gagcagtggg catgaacatt tcttcgtcta ccttcgcctc ctatcgttac 60aaacgtcacc gctttccagc cgagattatc gctcacgcgg tttggctgta cttccggttt 120ccattgagcc tgcggcatgt cgaggatttg ctggccgaac gcggtattga cgtttcgttc 180cagacagtat cggaatgggc cgccaaattt ggctccgagt ttgctgcgtt gcttcgccgg 240cgctcaaagg gcggctttgc cgataaatgg cacctcgatg agatggtggt aaccatcaaa 300ggccggaaat acggctctgt cgcaaagatt ccgtttgtgc tggaaataag ggagatagtt 360ttcagcgaga cttttgatga gcgatttcaa gtggcgtcat tttcagggcg aggtgatctt 420gtgggcagtt cgctggtatt gccgctatgg ggtgagctac cgcgatcttg a 47117156PRTA. vitis strain F2/5 17Met Tyr Asp Arg Ala Val Gly Met Asn Ile Ser Ser Ser Thr Phe Ala 1 5 10 15 Ser Tyr Arg Tyr Lys Arg His Arg Phe Pro Ala Glu Ile Ile Ala His 20 25 30 Ala Val Trp Leu Tyr Phe Arg Phe Pro Leu Ser Leu Arg His Val Glu 35 40 45 Asp Leu Leu Ala Glu Arg Gly Ile Asp Val Ser Phe Gln Thr Val Ser 50 55 60 Glu Trp Ala Ala Lys Phe Gly Ser Glu Phe Ala Ala Leu Leu Arg Arg 65 70 75 80 Arg Ser Lys Gly Gly Phe Ala Asp Lys Trp His Leu Asp Glu Met Val 85 90 95 Val Thr Ile Lys Gly Arg Lys Tyr Gly Ser Val Ala Lys Ile Pro Phe 100 105 110 Val Leu Glu Ile Arg Glu Ile Val Phe Ser Glu Thr Phe Asp Glu Arg 115 120 125 Phe Gln Val Ala Ser Phe Ser Gly Arg Gly Asp Leu Val Gly Ser Ser 130 135 140 Leu Val Leu Pro Leu Trp Gly Glu Leu Pro Arg Ser 145 150 155 181173DNAA. vitis strain F2/5gene(1)..(1173)P1389 18gtgataaaac cagccaccac tctctttgcg gctctgctgt cgtgtcttgt cgcgcagatg 60ccatctgctg tggcacagga gccagcggct caaacatcca atgcagcaac aacgccgcat 120cgcatcaccg tgacagacat tgctggtcgg caggtgtcat tgcctgttcc tgtccatcgc 180atgttgttgg gcgaagggcg gcagctctat cttgtcgcgt cattggagtc cgagaatcct 240ctggcgtttc tggcaggatg gaaaaatgac ctcattgaag ccgaccccgc aacctatgcc 300caatatgtcg cgaagtttcc gaaactggcc caactgccaa cctttggcgg caaggaagca 360ggtctgattg atcttgagtc tgccattgtt caaaagcctg atgttgtgct tttgaatctc 420gaaacccagc gtgccagcaa agagtccgaa tatatcgaaa agctggcatc actgggaata 480ccggttcttt atatcgattt ccggcatcaa ccccttcaca ataccgaacc aacaatccgc 540cttttgggcg agatcatggg acgtgaaaag cgcgccgagg aaattattgc cttcaggaaa 600aaggcattgt cgcaagtgac ggatgtgatt gaacaaaaaa agcccgcacg accgaatgtc 660tttgtcgagc ggattggcgg ttatgcggat gattgctgcc tcacctttgg caatgagaat 720tttggtcgct atgtcgaaat cgccggcggg cacaatatcg gcggtgattt tcttcccgga 780accttcggac aattgaaccc ggaacaggtg attgctgccg acccggatca ggttgtggtc 840acgagcgcca attgggaagc ttatgttccg ggtggacact ggatacccct ggggccgggt 900gccaacagac aggaagccga acgaaaactg gaatggttca cgacaagaaa tgcctatatt 960ggcaccaaag cggtcaaaaa ccgcagtttc catggcatat ggcatcagtt ctacaatagc 1020ccttatgaat tcatcgccat acaacaaatc gcaaaatggc ttcatccgga tttgttcgcc 1080gatctcgatc cagacgagac attcagggaa tatcatcgcc tttttctgcc aatcgaatac 1140aagtcaggct atagtataag ccttgcgccc tga 117319390PRTA. vitis strain F2/5 19Met Ile Lys Pro Ala Thr Thr Leu Phe Ala Ala Leu Leu Ser Cys Leu 1 5 10 15 Val Ala Gln Met Pro Ser Ala Val Ala Gln Glu Pro Ala Ala Gln Thr 20 25 30 Ser Asn Ala Ala Thr Thr Pro His Arg Ile Thr Val Thr Asp Ile Ala 35 40 45 Gly Arg Gln Val Ser Leu Pro Val Pro Val His Arg Met Leu Leu Gly 50 55 60 Glu Gly Arg Gln Leu Tyr Leu Val Ala Ser Leu Glu Ser Glu Asn Pro 65 70 75 80 Leu Ala Phe Leu Ala Gly Trp Lys Asn Asp Leu Ile Glu Ala Asp Pro 85 90 95 Ala Thr Tyr Ala Gln Tyr Val Ala Lys Phe Pro Lys Leu Ala Gln Leu 100 105 110 Pro Thr Phe Gly Gly Lys Glu Ala Gly Leu Ile Asp Leu Glu Ser Ala 115 120 125 Ile Val Gln Lys Pro Asp Val Val Leu Leu Asn Leu Glu Thr Gln Arg 130 135 140 Ala Ser Lys Glu Ser Glu Tyr Ile Glu Lys Leu Ala Ser Leu Gly Ile 145 150 155 160 Pro Val Leu Tyr Ile Asp Phe Arg His Gln Pro Leu His Asn Thr Glu 165 170 175 Pro Thr Ile Arg Leu Leu Gly Glu Ile Met Gly Arg Glu Lys Arg Ala 180 185 190 Glu Glu Ile Ile Ala Phe Arg Lys Lys Ala Leu Ser Gln Val Thr Asp 195 200 205 Val Ile Glu Gln Lys Lys Pro Ala Arg Pro Asn Val Phe Val Glu Arg 210 215 220 Ile Gly Gly Tyr Ala Asp Asp Cys Cys Leu Thr Phe Gly Asn Glu Asn 225 230 235 240 Phe Gly Arg Tyr Val Glu Ile Ala Gly Gly His Asn Ile Gly Gly Asp 245 250 255 Phe Leu Pro Gly Thr Phe Gly Gln Leu Asn Pro Glu Gln Val Ile Ala 260 265 270 Ala Asp Pro Asp Gln Val Val Val Thr Ser Ala Asn Trp Glu Ala Tyr 275 280 285 Val Pro Gly Gly His Trp Ile Pro Leu Gly Pro Gly Ala Asn Arg Gln 290 295 300 Glu Ala Glu Arg Lys Leu Glu Trp Phe Thr Thr Arg Asn Ala Tyr Ile 305 310 315 320 Gly Thr Lys Ala Val Lys Asn Arg Ser Phe His Gly Ile Trp His Gln 325 330 335 Phe Tyr Asn Ser Pro Tyr Glu Phe Ile Ala Ile Gln Gln Ile Ala Lys 340 345 350 Trp Leu His Pro Asp Leu Phe Ala Asp Leu Asp Pro Asp Glu Thr Phe 355 360 365 Arg Glu Tyr His Arg Leu Phe Leu Pro Ile Glu Tyr Lys Ser Gly Tyr 370 375 380 Ser Ile Ser Leu Ala Pro 385 390 201206DNAA. vitis strain F2/5gene(1)..(1206)P1390 20atgctcaggg caagcacgcc tgtgtttctg ctgctgtttg cgctccagtt catttccatg 60ggagccatgg aaatgagcgg tcctttctgg ccgctgcaga tcagggtact cagcccgtcg 120gatacggcgt tcggattggc aagtatcggg gtttatattt gccccatgct tggcatttcc 180ctcaccagcg cattctgggg gcgtatgggt gaccgctatg gcaatcgctt gatgatggtt 240cgggccttgg cggggttggc gatcacccag cttctgattg cgcttgccca agatgtctgg 300gtcatcctgt ttctgcggtt cttacagggg gctttcgccg gctatattgc gcccgcgcaa 360gcctatgccg tgcaggtcaa tagcgggcgt gatcgcgcca gtctgttcgc ctggttgcag 420gtggcgacca atgtcggttc gctgggcggc gcatttgcgg gtggtctcat tctggatgca 480atgccattcg cggccgttaa tatcacggcc ggggccgtat gtggtctatg tgcccttgtt 540gcctgggcaa cattgcccat cccgcccatc agacagaatg caccgcgctc cgcttcaagc 600gcgctcaccc cgggccatcc agaaagttct gcaccagtcg tcgggttgct ggttttgatt 660tgcgtattgt tggccagccg aatggtgctg caagtgccgt tttcgctcta catgacccaa 720gctttcggag ccaaccactg gattattggc ctcagctacg ggctcatggc gtttggtttt 780gtgattggcg cccccctctg ggcgcgtctg ttcaaaatgc gcagccttgg ctttgtgttg 840gcggcaaaca ccgcgatcgc tctggcctgc gcaagcatcg ctttgatagc gggctacaca 900accctcgttc ccgtttttgc aggattatac tttgtctggg gcattctgct tggtggtacg 960acaccggttc ttacctcgcg tctttcccag tccacagcct tcaatcggca aggtgaaatt 1020ttgggccgtg cgcaaagctg gcagcaaatc gcttccattc tgggtatagc agccggtgtt 1080gggtccatgc agctttgggg tgctgatacc gcattttccc tggtatcggc gtgttacatc 1140ctgtcatttt ttatcggcct gtcgctcttg ctcaaaagcg gccccttacc caaccgcata 1200ggataa 120621401PRTA. vitis strain F2/5 21Met Leu Arg Ala Ser Thr Pro Val Phe Leu Leu Leu Phe Ala Leu Gln 1 5 10 15 Phe Ile Ser Met Gly Ala Met Glu Met Ser Gly Pro Phe Trp Pro Leu 20 25 30 Gln Ile Arg Val Leu Ser Pro Ser Asp Thr Ala Phe Gly Leu Ala Ser 35 40 45 Ile Gly Val Tyr Ile Cys Pro Met Leu Gly Ile Ser Leu Thr Ser Ala 50 55 60 Phe Trp Gly Arg Met Gly Asp Arg Tyr Gly Asn Arg Leu Met Met Val 65 70 75 80 Arg Ala Leu Ala Gly Leu Ala Ile Thr Gln Leu Leu Ile Ala Leu Ala 85 90 95 Gln Asp Val Trp Val Ile Leu Phe Leu Arg Phe Leu Gln Gly Ala Phe 100 105 110 Ala Gly Tyr Ile Ala Pro Ala Gln Ala Tyr Ala Val Gln Val Asn Ser 115 120 125 Gly Arg Asp Arg Ala Ser Leu Phe Ala Trp Leu Gln Val Ala Thr Asn 130 135 140 Val Gly Ser Leu Gly Gly Ala Phe Ala Gly Gly Leu Ile Leu Asp Ala 145 150 155 160 Met Pro Phe Ala Ala Val Asn Ile Thr Ala Gly Ala Val Cys Gly Leu 165 170 175 Cys Ala Leu Val Ala Trp Ala Thr Leu Pro Ile Pro Pro Ile Arg Gln 180 185 190 Asn Ala Pro Arg Ser Ala Ser Ser Ala Leu Thr Pro Gly His Pro Glu 195 200 205 Ser Ser Ala Pro Val Val Gly Leu Leu Val Leu Ile Cys Val Leu Leu 210 215 220 Ala Ser Arg Met Val Leu Gln Val Pro Phe Ser Leu Tyr Met Thr Gln 225 230 235 240 Ala Phe Gly Ala Asn His Trp Ile Ile Gly Leu Ser Tyr Gly Leu Met 245 250 255 Ala Phe Gly Phe Val Ile Gly Ala Pro Leu Trp Ala Arg Leu Phe Lys 260 265 270 Met Arg Ser Leu Gly Phe Val Leu Ala Ala Asn Thr Ala Ile Ala Leu 275 280 285 Ala Cys Ala Ser Ile Ala Leu Ile Ala Gly Tyr Thr Thr Leu Val Pro 290 295 300 Val Phe Ala Gly Leu Tyr Phe Val Trp Gly Ile Leu Leu Gly Gly Thr 305 310 315 320 Thr Pro Val Leu Thr Ser Arg Leu Ser Gln Ser Thr Ala Phe Asn Arg 325 330 335 Gln Gly Glu Ile Leu Gly Arg Ala Gln Ser Trp Gln Gln Ile Ala Ser 340 345 350 Ile Leu Gly Ile Ala Ala Gly Val Gly Ser Met Gln Leu Trp Gly Ala 355 360 365 Asp Thr Ala Phe Ser Leu Val Ser Ala Cys Tyr Ile Leu Ser Phe Phe 370 375 380 Ile Gly Leu Ser Leu Leu Leu Lys Ser Gly Pro Leu Pro Asn Arg Ile 385 390 395 400 Gly 221893DNAA. vitis strain F2/5gene(1)..(1893)P1391 22atgacccatt tgctttcgcc tttgtttgac atccatgcgc agaccgatga ccagccgttg 60cgggattttg ccgttgccaa tgcaatcagt cgccttgttc gctgcctgtt tgcagaacgt 120cttctggctc ctgaagcgtt gctctggtcg cgtgacaagc atcaagcctg gttgccgctg 180tggaacacgc gccgtgtgct gcattttaaa catttgcatg cggcacccgc tggcaccttg 240caaaaccgtg gcgagattga aattctcgac gccgacggcg gccatgcccg gattgaccat 300cccgatgctt tgctgcggga agtgttcgct tcgttgtcca tctctccggc ccctgatggg 360cttgaggttt tgatgcgcga tatcgccgac agcatcaaca atgatatgct ggcccgccga 420caccggcagg tctgggctgc gagcctgaat gagaagatca aacaatccgg tgcggtggat 480tttctgggct atctctacgg tcatttgcct gcacatctgg cggcaatgct gattgaccag 540tggggtgcgc tcgaaggtca tccgttttat ccgacatgga aggcaaaacc gagtttgcag 600cccgaagagg ttgttgccct ttcgcctgaa ttcggggcca aggtgccggt ccggattgcg 660gccttgcgtc ggggttgggc ctatgtggaa aaaatgccac atgtgactga ttacaatgac 720tggttttctg cccagtttcc cgaattgttt gccgcgtgga aagaccatct gagcgcacag 780ggcaaatcag cgcaagactg gattcctttg cccatccacg gatggcacct cgagcatttt 840gtccgccgcg aatttggaca ggaaatcgcc tctggcatct tgctgttgga cgggccagac 900attgacacct cgccatccat gtcatttcgg accatgttac cggacgatga aacgttgcgc 960cctttcatca agttgccggt tgccatctgg ctcaccagcg agcagcgcag cttgcaggcc 1020aaatccattc acatggggcc tcggctttcc acgttgatca ccgatattct ggccaatgaa 1080ccggacatgg ccgcacaatt ggaaatcttc accgaagaat tgggagccat tctgcacgat 1140cccgataccg gcgatgagca tcgcgggcga tttttgtcgg tggtctatcg caacgcccag 1200cctttaaagc gcgaggatgg acgctatgcg gtgacggtcg ccgcacttct aagtgcaagt 1260cccaaggatg gacgaccatt gatctgcgag ctgattgccc gttacggcaa tgaggaacag 1320gcaagcgttg tcggcttttt ccgccggtat gttgaggtgg tggttcggcc tgcgttggcg 1380atgtatcttc tttatggcat cgcttttgag gcccatcagc aaaacaccac catcgtgttt 1440gatccttccg gttctgcgga aaagctgatc attcgggatt ttggtgatgg ccgcagcttt 1500gcgcctctgc tcaaacagcg tggctatcac ctcaaaccct ttcagcgtgc tgggattttg 1560cccaccacct ttgacgatga cattgggctg gttcgctcgt ttttgatcga cgcctgcttt 1620atatgccatc tgcatgaaat tgctctgtgc ctgacccagc actatagtat cggcaatgat 1680aggctctgga cagtgatcaa agaggtaacg caagaggcgt ttgaaacatt gcggcctcgt 1740gtgtcttcgg acacgttctg gcaagaagaa cgagatgccc tgcttgaaaa aaaatggccc 1800acccggtcgg ttctcaccat gcatctccag cgatatagcg actatcgggt ggagcatgag 1860ctgcccaatc ctctggttgg ccgtgaatgc tga 189323630PRTA. vitis strain F2/5 23Met Thr His Leu Leu Ser Pro Leu Phe Asp Ile His Ala Gln Thr Asp 1 5 10 15 Asp Gln Pro Leu Arg Asp Phe Ala Val Ala Asn Ala Ile Ser Arg Leu 20 25 30 Val Arg Cys Leu Phe Ala Glu Arg Leu Leu Ala Pro Glu Ala Leu Leu 35 40 45 Trp Ser Arg Asp Lys His Gln Ala Trp Leu Pro Leu Trp Asn Thr Arg 50 55 60 Arg Val Leu His Phe Lys His Leu His Ala Ala Pro Ala Gly Thr Leu 65 70 75 80 Gln Asn Arg Gly Glu Ile Glu Ile Leu Asp Ala Asp Gly Gly His Ala 85 90 95 Arg Ile Asp His Pro Asp Ala Leu Leu Arg Glu Val Phe Ala Ser Leu 100 105 110 Ser Ile Ser Pro Ala Pro Asp Gly Leu Glu Val Leu Met Arg Asp Ile 115 120 125 Ala Asp Ser Ile Asn Asn Asp Met Leu Ala Arg Arg His Arg Gln Val 130 135 140 Trp Ala Ala Ser Leu Asn Glu Lys Ile Lys Gln Ser Gly Ala Val Asp 145 150 155 160 Phe Leu Gly Tyr Leu Tyr Gly His Leu Pro Ala His Leu Ala Ala Met 165 170 175 Leu Ile Asp Gln Trp Gly Ala Leu Glu Gly His Pro Phe Tyr Pro Thr 180 185 190 Trp Lys Ala Lys Pro Ser Leu Gln Pro Glu Glu Val Val Ala Leu Ser 195 200

205 Pro Glu Phe Gly Ala Lys Val Pro Val Arg Ile Ala Ala Leu Arg Arg 210 215 220 Gly Trp Ala Tyr Val Glu Lys Met Pro His Val Thr Asp Tyr Asn Asp 225 230 235 240 Trp Phe Ser Ala Gln Phe Pro Glu Leu Phe Ala Ala Trp Lys Asp His 245 250 255 Leu Ser Ala Gln Gly Lys Ser Ala Gln Asp Trp Ile Pro Leu Pro Ile 260 265 270 His Gly Trp His Leu Glu His Phe Val Arg Arg Glu Phe Gly Gln Glu 275 280 285 Ile Ala Ser Gly Ile Leu Leu Leu Asp Gly Pro Asp Ile Asp Thr Ser 290 295 300 Pro Ser Met Ser Phe Arg Thr Met Leu Pro Asp Asp Glu Thr Leu Arg 305 310 315 320 Pro Phe Ile Lys Leu Pro Val Ala Ile Trp Leu Thr Ser Glu Gln Arg 325 330 335 Ser Leu Gln Ala Lys Ser Ile His Met Gly Pro Arg Leu Ser Thr Leu 340 345 350 Ile Thr Asp Ile Leu Ala Asn Glu Pro Asp Met Ala Ala Gln Leu Glu 355 360 365 Ile Phe Thr Glu Glu Leu Gly Ala Ile Leu His Asp Pro Asp Thr Gly 370 375 380 Asp Glu His Arg Gly Arg Phe Leu Ser Val Val Tyr Arg Asn Ala Gln 385 390 395 400 Pro Leu Lys Arg Glu Asp Gly Arg Tyr Ala Val Thr Val Ala Ala Leu 405 410 415 Leu Ser Ala Ser Pro Lys Asp Gly Arg Pro Leu Ile Cys Glu Leu Ile 420 425 430 Ala Arg Tyr Gly Asn Glu Glu Gln Ala Ser Val Val Gly Phe Phe Arg 435 440 445 Arg Tyr Val Glu Val Val Val Arg Pro Ala Leu Ala Met Tyr Leu Leu 450 455 460 Tyr Gly Ile Ala Phe Glu Ala His Gln Gln Asn Thr Thr Ile Val Phe 465 470 475 480 Asp Pro Ser Gly Ser Ala Glu Lys Leu Ile Ile Arg Asp Phe Gly Asp 485 490 495 Gly Arg Ser Phe Ala Pro Leu Leu Lys Gln Arg Gly Tyr His Leu Lys 500 505 510 Pro Phe Gln Arg Ala Gly Ile Leu Pro Thr Thr Phe Asp Asp Asp Ile 515 520 525 Gly Leu Val Arg Ser Phe Leu Ile Asp Ala Cys Phe Ile Cys His Leu 530 535 540 His Glu Ile Ala Leu Cys Leu Thr Gln His Tyr Ser Ile Gly Asn Asp 545 550 555 560 Arg Leu Trp Thr Val Ile Lys Glu Val Thr Gln Glu Ala Phe Glu Thr 565 570 575 Leu Arg Pro Arg Val Ser Ser Asp Thr Phe Trp Gln Glu Glu Arg Asp 580 585 590 Ala Leu Leu Glu Lys Lys Trp Pro Thr Arg Ser Val Leu Thr Met His 595 600 605 Leu Gln Arg Tyr Ser Asp Tyr Arg Val Glu His Glu Leu Pro Asn Pro 610 615 620 Leu Val Gly Arg Glu Cys 625 630 241137DNAA. vitis strain F2/5gene(1)..(1137)P1392 24atgagggtcg ccatttgcgg cgcaggccgc accgggcatt tgaatgccgt gctttttaag 60caaaatccgc aggtccatct ctccgttttg acggagaatg acactcttgc cgccaattgg 120gctggaaatc ctgctccatg gcaggctttt ttgccggatg gtcagggtat gtccgggcgt 180cctgattacg ttggcaacaa ccccgataaa gcggttggga acgctgatct gattgtcatt 240acccagccag cccatgccag atccgctgtt ttgcagcgga tcgcaggcag tatctccagc 300accaagcagg ttttcgtggg tgctattccg ggcttttgcg gttttgactg gctggctgcc 360gatcacttag gcgcaaggga taatgtggtc atctggggtt tgaaggatgt gccgcatatc 420gcctacgacc ttgttgccgg acagagcgtt cgcatgggcg gcgctaaagc ccatttgttt 480gctggccttc atcggcggga atccgcccaa agtgccaagg ctctgcaaac aatgctgacc 540gcactttaca atgcgcctgt aacccttttg caggactatc tggaaatcac cctgacaccc 600ggcaatgcgc tgatgcatcc agcggtgctc tacggcctgc tggggccggg cgctccctgg 660gagcgtaaac cgttctccga gccgctgtgc tggtggagtg attgccctga ggaaggcgcg 720cgcttgctcg aaatgttcga tgccgaaaat caggccatat gtcaggcgat tgcgacccga 780accggtcttg atctcagctc cgtcaaacct ttgcggcagg aactgattga agcctatggc 840gatcagattg ccgatagtca aacgatgtat tccctgctca gaacaaacag cgcctatgcg 900ggaattcaat tgccgctgat ccccaatcat catggtcaag gctggttgat cgatctgcaa 960agccgtgcct ttcatgagga tgttgcatgc gggcaggcat tgctggtcaa gatcgcagag 1020cggctggcgg taccgattcc aaccatctcg aaaacctaca cgtgggcgcg ccactatcat 1080ggtgctttgc cggatggatt aagcccggtt cctgatgatt ggctggaggc tgcctga 113725378PRTA. vitis strain F2/5 25Met Arg Val Ala Ile Cys Gly Ala Gly Arg Thr Gly His Leu Asn Ala 1 5 10 15 Val Leu Phe Lys Gln Asn Pro Gln Val His Leu Ser Val Leu Thr Glu 20 25 30 Asn Asp Thr Leu Ala Ala Asn Trp Ala Gly Asn Pro Ala Pro Trp Gln 35 40 45 Ala Phe Leu Pro Asp Gly Gln Gly Met Ser Gly Arg Pro Asp Tyr Val 50 55 60 Gly Asn Asn Pro Asp Lys Ala Val Gly Asn Ala Asp Leu Ile Val Ile 65 70 75 80 Thr Gln Pro Ala His Ala Arg Ser Ala Val Leu Gln Arg Ile Ala Gly 85 90 95 Ser Ile Ser Ser Thr Lys Gln Val Phe Val Gly Ala Ile Pro Gly Phe 100 105 110 Cys Gly Phe Asp Trp Leu Ala Ala Asp His Leu Gly Ala Arg Asp Asn 115 120 125 Val Val Ile Trp Gly Leu Lys Asp Val Pro His Ile Ala Tyr Asp Leu 130 135 140 Val Ala Gly Gln Ser Val Arg Met Gly Gly Ala Lys Ala His Leu Phe 145 150 155 160 Ala Gly Leu His Arg Arg Glu Ser Ala Gln Ser Ala Lys Ala Leu Gln 165 170 175 Thr Met Leu Thr Ala Leu Tyr Asn Ala Pro Val Thr Leu Leu Gln Asp 180 185 190 Tyr Leu Glu Ile Thr Leu Thr Pro Gly Asn Ala Leu Met His Pro Ala 195 200 205 Val Leu Tyr Gly Leu Leu Gly Pro Gly Ala Pro Trp Glu Arg Lys Pro 210 215 220 Phe Ser Glu Pro Leu Cys Trp Trp Ser Asp Cys Pro Glu Glu Gly Ala 225 230 235 240 Arg Leu Leu Glu Met Phe Asp Ala Glu Asn Gln Ala Ile Cys Gln Ala 245 250 255 Ile Ala Thr Arg Thr Gly Leu Asp Leu Ser Ser Val Lys Pro Leu Arg 260 265 270 Gln Glu Leu Ile Glu Ala Tyr Gly Asp Gln Ile Ala Asp Ser Gln Thr 275 280 285 Met Tyr Ser Leu Leu Arg Thr Asn Ser Ala Tyr Ala Gly Ile Gln Leu 290 295 300 Pro Leu Ile Pro Asn His His Gly Gln Gly Trp Leu Ile Asp Leu Gln 305 310 315 320 Ser Arg Ala Phe His Glu Asp Val Ala Cys Gly Gln Ala Leu Leu Val 325 330 335 Lys Ile Ala Glu Arg Leu Ala Val Pro Ile Pro Thr Ile Ser Lys Thr 340 345 350 Tyr Thr Trp Ala Arg His Tyr His Gly Ala Leu Pro Asp Gly Leu Ser 355 360 365 Pro Val Pro Asp Asp Trp Leu Glu Ala Ala 370 375 261011DNAA. vitis strain F2/5gene(1)..(1011)P1393 26atgctctata caaccgtcac gcaattgatt ggccagaccc ccctgatcgc gcttgatctg 60gcaacaagca atgcaaaact tttgctcaaa atggagaaga acaatcctgg cggctcaatg 120aaagaccgca tggcgcgcag catggtggtt gcggccttgg cggatgggcg cctgccgcgt 180ggcggcacga tcgttgaatc ctcatcaggc aacacgggca caggtctggc tcttgcggcg 240ctggagtttg gtcttcgctt tatcgcggtc gtggatcatc acgccgcgcc ggataaaatc 300cggatgatgc gggccttggg tgccgaaatt cgctatgttg aaggcgacta tcgcgaagat 360gaagtggccg tcgttgaaag gcagagactg gccgcccagc tcggcgcgca actgcctggt 420gcactgttca tgaaccagtc cgacaatccc gccaatcctg acggctatgc cggatttgtg 480gatgagttga tggcacaact gcctgatgga ttggatgcct ttgttggttg cgtgggtacg 540ggtgggtcga tgacgggcat atcccagcgc ctcaagcgcc tcaatcccgc catcaagaca 600attgcggttg agcctgctgg ttccattgtt tttggtaagc cggggcattc ctattaccaa 660tctggcacag gtacgcccgc aggcgatgaa gtcggcaagg ttctggatta tagctgcatt 720gatgaaggtg ttcaggtcac cgatacgcaa gcatttgaaa cggcacgcta tattgcccga 780cgcaaaggta ttctcgttgg tggatcaacc gggggtacaa tttacaaagc tctcgaattc 840atcgcatccg gtaaattgag cggcacaatt gttacagccg tcgcagatgg gggtgaaaaa 900tacctggcat ccgtgtttga cgatgagtgg atggaaaagc gcgggcttct caatccggca 960atcgcagctc aattggatgg ttggttcaat ggcttggcag gtgcggcatg a 101127336PRTA. vitis strain F2/5 27Met Leu Tyr Thr Thr Val Thr Gln Leu Ile Gly Gln Thr Pro Leu Ile 1 5 10 15 Ala Leu Asp Leu Ala Thr Ser Asn Ala Lys Leu Leu Leu Lys Met Glu 20 25 30 Lys Asn Asn Pro Gly Gly Ser Met Lys Asp Arg Met Ala Arg Ser Met 35 40 45 Val Val Ala Ala Leu Ala Asp Gly Arg Leu Pro Arg Gly Gly Thr Ile 50 55 60 Val Glu Ser Ser Ser Gly Asn Thr Gly Thr Gly Leu Ala Leu Ala Ala 65 70 75 80 Leu Glu Phe Gly Leu Arg Phe Ile Ala Val Val Asp His His Ala Ala 85 90 95 Pro Asp Lys Ile Arg Met Met Arg Ala Leu Gly Ala Glu Ile Arg Tyr 100 105 110 Val Glu Gly Asp Tyr Arg Glu Asp Glu Val Ala Val Val Glu Arg Gln 115 120 125 Arg Leu Ala Ala Gln Leu Gly Ala Gln Leu Pro Gly Ala Leu Phe Met 130 135 140 Asn Gln Ser Asp Asn Pro Ala Asn Pro Asp Gly Tyr Ala Gly Phe Val 145 150 155 160 Asp Glu Leu Met Ala Gln Leu Pro Asp Gly Leu Asp Ala Phe Val Gly 165 170 175 Cys Val Gly Thr Gly Gly Ser Met Thr Gly Ile Ser Gln Arg Leu Lys 180 185 190 Arg Leu Asn Pro Ala Ile Lys Thr Ile Ala Val Glu Pro Ala Gly Ser 195 200 205 Ile Val Phe Gly Lys Pro Gly His Ser Tyr Tyr Gln Ser Gly Thr Gly 210 215 220 Thr Pro Ala Gly Asp Glu Val Gly Lys Val Leu Asp Tyr Ser Cys Ile 225 230 235 240 Asp Glu Gly Val Gln Val Thr Asp Thr Gln Ala Phe Glu Thr Ala Arg 245 250 255 Tyr Ile Ala Arg Arg Lys Gly Ile Leu Val Gly Gly Ser Thr Gly Gly 260 265 270 Thr Ile Tyr Lys Ala Leu Glu Phe Ile Ala Ser Gly Lys Leu Ser Gly 275 280 285 Thr Ile Val Thr Ala Val Ala Asp Gly Gly Glu Lys Tyr Leu Ala Ser 290 295 300 Val Phe Asp Asp Glu Trp Met Glu Lys Arg Gly Leu Leu Asn Pro Ala 305 310 315 320 Ile Ala Ala Gln Leu Asp Gly Trp Phe Asn Gly Leu Ala Gly Ala Ala 325 330 335 281398DNAA. vitis strain F2/5gene(1)..(1398)P1394 28atgagacttg gctccaagca atctcacact ctgacgcccc tgattcatga cgaggtcgca 60gattttattg aaaacaagca tgaacagttg cgggaaggat tcaacagatt tggctcgccg 120ctccatttga tctggcctga tctgctggcc gataatcttc tggctttgca ggccatattg 180agcggcagtc aagttccatg cagaatttat tatggtgtga aggtcaacaa gtctgtgaat 240cttatgggag ctgcccttca ggctgaagga ggcgttgatg tctccagcct gtacgagttg 300caggatgcct tggcattggg tgcaaagggt gccgatattg ttgcgaccgg tcctgccaaa 360acatcgacat ttcatagagc gttgatggcc tgcaaggccc tgatttccat cgattccgtt 420gaggaattcg atgatatttt gaaggaattg ccaagcgccg gtgaccctca gcccgtgctt 480ttgagattaa ggccccaaaa tcaagggcaa agccgcttcg gcatggatgc tcaagacgtt 540gtttcgtgcc tgcatcgttt ggcaggcacc ccagccctac gatttgatgg aatccatttc 600cacttgagtg gttatagcgt taaggcgcgc atcgaagcat tgaatgaagc agcggatctc 660attgccaaag cgcgcggcat ggggctttct ccccgcatga tcgatattgg tggtgggttg 720ccggtgcaat atgtcgatca cccgacctac gccgcctttc tggaggcgca aacggcgcag 780gattatcgca ccaaccatct gccacagtct ttttatccct atggaggacc tgtttgcgca 840acggagtggt taactcacct gttgcaatcc aaaatgcagg acgggcgcga tgtcgcaaca 900tatttccgtc aggaaaatct tacgctgggg atggagccgg ggcgggccct tgcggatcag 960actgcaatca cggctttcag agtgacgaga gtgaaggcac tgggtgcgca gtcagcagca 1020atttttgtgg agggcagcag cttcagtgca tgcgaaacat ggttcgcatc agaatttctg 1080atcgatccca ttgtggtgcc gcacgggccg gaagctgcac gcaccactcc tgtgcgcgcc 1140tatcttgccg gacatagctg ccttgatgaa gatattgtct ccaaccgctg gctcgacttt 1200ccgattaccc cgcaagctgg tgatttgctc atctatgtca ataccggcgg ctatcagatg 1260gatctgatgg aaaaccagtt tcaccgccac cccatgccaa tccgcttatg tgtcaggagg 1320accgcagatg gactgtccga acttattccg gatacctgtg aggaggtact atgctctata 1380caaccgtcac gcaattga 139829465PRTA. vitis strain F2/5 29Met Arg Leu Gly Ser Lys Gln Ser His Thr Leu Thr Pro Leu Ile His 1 5 10 15 Asp Glu Val Ala Asp Phe Ile Glu Asn Lys His Glu Gln Leu Arg Glu 20 25 30 Gly Phe Asn Arg Phe Gly Ser Pro Leu His Leu Ile Trp Pro Asp Leu 35 40 45 Leu Ala Asp Asn Leu Leu Ala Leu Gln Ala Ile Leu Ser Gly Ser Gln 50 55 60 Val Pro Cys Arg Ile Tyr Tyr Gly Val Lys Val Asn Lys Ser Val Asn 65 70 75 80 Leu Met Gly Ala Ala Leu Gln Ala Glu Gly Gly Val Asp Val Ser Ser 85 90 95 Leu Tyr Glu Leu Gln Asp Ala Leu Ala Leu Gly Ala Lys Gly Ala Asp 100 105 110 Ile Val Ala Thr Gly Pro Ala Lys Thr Ser Thr Phe His Arg Ala Leu 115 120 125 Met Ala Cys Lys Ala Leu Ile Ser Ile Asp Ser Val Glu Glu Phe Asp 130 135 140 Asp Ile Leu Lys Glu Leu Pro Ser Ala Gly Asp Pro Gln Pro Val Leu 145 150 155 160 Leu Arg Leu Arg Pro Gln Asn Gln Gly Gln Ser Arg Phe Gly Met Asp 165 170 175 Ala Gln Asp Val Val Ser Cys Leu His Arg Leu Ala Gly Thr Pro Ala 180 185 190 Leu Arg Phe Asp Gly Ile His Phe His Leu Ser Gly Tyr Ser Val Lys 195 200 205 Ala Arg Ile Glu Ala Leu Asn Glu Ala Ala Asp Leu Ile Ala Lys Ala 210 215 220 Arg Gly Met Gly Leu Ser Pro Arg Met Ile Asp Ile Gly Gly Gly Leu 225 230 235 240 Pro Val Gln Tyr Val Asp His Pro Thr Tyr Ala Ala Phe Leu Glu Ala 245 250 255 Gln Thr Ala Gln Asp Tyr Arg Thr Asn His Leu Pro Gln Ser Phe Tyr 260 265 270 Pro Tyr Gly Gly Pro Val Cys Ala Thr Glu Trp Leu Thr His Leu Leu 275 280 285 Gln Ser Lys Met Gln Asp Gly Arg Asp Val Ala Thr Tyr Phe Arg Gln 290 295 300 Glu Asn Leu Thr Leu Gly Met Glu Pro Gly Arg Ala Leu Ala Asp Gln 305 310 315 320 Thr Ala Ile Thr Ala Phe Arg Val Thr Arg Val Lys Ala Leu Gly Ala 325 330 335 Gln Ser Ala Ala Ile Phe Val Glu Gly Ser Ser Phe Ser Ala Cys Glu 340 345 350 Thr Trp Phe Ala Ser Glu Phe Leu Ile Asp Pro Ile Val Val Pro His 355 360 365 Gly Pro Glu Ala Ala Arg Thr Thr Pro Val Arg Ala Tyr Leu Ala Gly 370 375 380 His Ser Cys Leu Asp Glu Asp Ile Val Ser Asn Arg Trp Leu Asp Phe 385 390 395 400 Pro Ile Thr Pro Gln Ala Gly Asp Leu Leu Ile Tyr Val Asn Thr Gly 405 410 415 Gly Tyr Gln Met Asp Leu Met Glu Asn Gln Phe His Arg His Pro Met 420 425 430 Pro Ile Arg Leu Cys Val Arg Arg Thr Ala Asp Gly Leu Ser Glu Leu 435 440 445 Ile Pro Asp Thr Cys Glu Glu Val Leu Cys Ser Ile Gln Pro Ser Arg 450 455 460 Asn 465 30501DNAA. vitis strain F2/5gene(1)..(501)P1395 30atgttaaaca tgatttatgt agtcatattt tcggaaatag cgattttcac cgaagggctg 60tattttatgc catatcattt tgcctctccc aaaaaacttg tcgacactga agaagtcgat 120caaagcgttg ttgagaattt gcgccaaacc attttacgtc agcagatctg gacacgccct 180attgcggtcg agcgcactca gtttctggtg atggatgggc atcaccggct tgaggtcgca 240aggcagctca atctcgctgt cattcctgtt gtcctcctgg attatgagcg tgtgtctgtc 300acgagttggc ggcctgagga atgcattaca ccagacgata tttttgccat ggcgcggtcc 360ggcaaaaaat ttccgatcaa aacgacacgt cacgtcttcg cggatgatta ccctgtatgt 420gatgtaccgc tcgcggtatt agccggaatt gtggctacca tggattcggc gatgcaaggc 480ggcagggcgg tgctgtcatg a 50131166PRTA. vitis strain F2/5 31Met Leu Asn Met Ile Tyr Val Val Ile Phe Ser Glu Ile Ala Ile Phe 1 5 10 15 Thr Glu Gly Leu Tyr Phe Met Pro Tyr

His Phe Ala Ser Pro Lys Lys 20 25 30 Leu Val Asp Thr Glu Glu Val Asp Gln Ser Val Val Glu Asn Leu Arg 35 40 45 Gln Thr Ile Leu Arg Gln Gln Ile Trp Thr Arg Pro Ile Ala Val Glu 50 55 60 Arg Thr Gln Phe Leu Val Met Asp Gly His His Arg Leu Glu Val Ala 65 70 75 80 Arg Gln Leu Asn Leu Ala Val Ile Pro Val Val Leu Leu Asp Tyr Glu 85 90 95 Arg Val Ser Val Thr Ser Trp Arg Pro Glu Glu Cys Ile Thr Pro Asp 100 105 110 Asp Ile Phe Ala Met Ala Arg Ser Gly Lys Lys Phe Pro Ile Lys Thr 115 120 125 Thr Arg His Val Phe Ala Asp Asp Tyr Pro Val Cys Asp Val Pro Leu 130 135 140 Ala Val Leu Ala Gly Ile Val Ala Thr Met Asp Ser Ala Met Gln Gly 145 150 155 160 Gly Arg Ala Val Leu Ser 165 322154DNAA. vitis strain F2/5gene(1)..(2154)P1396 32atgatgggtt tgaaaagtgg cgttgccatg ggtgcaattc tggctgctct cggcactgtt 60gcgcaggcag aagaggacac ggttctcaag ccaattgttg ttgagggaca atcctcatcg 120cccaacgcaa ccataggcaa acagagcgaa ccttatgcag ggggcatggt caccggtagc 180gctcgattgg gcagtctggg caaccgcagt ttcatggata tgcctttcag caccagcgga 240tatactgcaa aagtcataga ggataaggga gcatcaacgg ttggtgatgt gatggagagc 300gatgcatcgg tgcgcaatac gcatccgtct ggcggcattg tcgactcttt ctatatccgt 360ggctttccca tcggtgacgg caattttggt gaaattgcgt ttgatggcat gttcggcgtc 420gcaccaaact atcgcgtttt taccgattat gcggaatccg tagaagtcct gaaagggcca 480acctccttcc tctatggcat ttctcccaat ggtggtgtgg gcggaaccat caatattgtt 540ccaaagcgtg ctctggatac ggacctgacc cgtgtcacca ccagctatga atcggatctt 600caggttggaa cgcatgtgga tatcagccgc cgctatggca gtgaacggca atttggcgtc 660cggctgaatg gcagcgttca aggtggtgat ggcactatcg acgatctttc tcgttttgcc 720tatgtcggtg cggttgcgct ggactacgaa ggagaaaatc tgcgcgcaac gctcgatgtc 780atcaatcaat atgaacatta tgacgcgcca cagcgtccat tctatcccac agccggcatt 840aaactgccga gtgcaccgga caatcgtctg aatgtgcagg agtcctggga atggtcagca 900acccgggagt tttcaacact ggggcgcgtt gaatatgacc tcagcgatga cgtgaccgtg 960tttggtgccg tcggtggtgg gaagtcaaac gtcgagcgtc tgtttggcac gcccacaatc 1020acggactctg ccggcaatgt cagcatcgta ccgcagcatt atatttttga tgtgcagaga 1080cgaaccgcag aaatcggcac tcgcggacag ttcgacaccg gcatcatcga gcactccgtg 1140acattgcagg ccaattatat gctgcaatgg ctgtcgcgag gctccaattc cggtacggcc 1200caaaccacca atctgtataa tccggttgat cgtgaggagc aatttgttgc caagccgtcc 1260agcgtaccga aagtaacgga aagcgaattc tacggtgtgg ctttatccga tacaatgtcc 1320atatgggatg agcgggcgca attgatggtt ggcgggcgct ttcaacacat cgattccgaa 1380aactacagct cgacaactgg cgctgtaaca tcctcttccg acgcgagcgc aataacaccc 1440atggttggtg tcgtggttaa gccgtgggaa aatgtatcgc tatatgcaaa ctatgcggaa 1500gggttgagca tcggggaaac ggctccgaca aacgccgtaa acgcaggcga aaccctcagc 1560ccctataaat ccaagcaata tgaagtggga accaagatag acaccggtcc ggtgacgctc 1620accgccagtc ttttccagat cgaaaagccg tttggtgttt tggagacgcg tggcagcgat 1680ctggttttcg aacgcggtgg cgaacagcgt aaccggggtc ttgaactttc agcgttcggc 1740gagttgaccg acaccgtgag actgcttggc ggcgttacgt tcatgcgggg cgagttgacg 1800aaaaccaaca atgcctcgac gcaaggcaat gaccccattg gcgtgccaaa agtcctcgta 1860aacctcggtg cagaatggga caccccattc ctgagcggct tcaccctgac gggcaacgtc 1920atccacaccg gcaaacaata tgccgataca gccaatatcc aaaaactacc ggcatggaca 1980cgtcttgatc tcggcgcgcg ctacaagaca acgatcaagg agcgacccgt taccttccgt 2040gccgaagtcg aaaacgtctt caacaagaat tactggtcag gtgtcgcaag ctttggcact 2100gtgacacaag gggccccgtt gaccgttaaa atatcgatga caaccgattt ttaa 215433716PRTA. vitis strain F2/5 33Met Met Gly Leu Lys Ser Gly Val Ala Met Gly Ala Ile Leu Ala Ala 1 5 10 15 Leu Gly Thr Val Ala Gln Ala Glu Glu Asp Thr Val Leu Lys Pro Ile 20 25 30 Val Val Glu Gly Gln Ser Ser Ser Pro Asn Ala Thr Ile Gly Lys Gln 35 40 45 Ser Glu Pro Tyr Ala Gly Gly Met Val Thr Gly Ser Ala Arg Leu Gly 50 55 60 Ser Leu Gly Asn Arg Ser Phe Met Asp Met Pro Phe Ser Thr Ser Gly 65 70 75 80 Tyr Thr Ala Lys Val Ile Glu Asp Lys Gly Ala Ser Thr Val Gly Asp 85 90 95 Val Met Glu Ser Asp Ala Ser Val Arg Asn Thr His Pro Ser Gly Gly 100 105 110 Ile Val Asp Ser Phe Tyr Ile Arg Gly Phe Pro Ile Gly Asp Gly Asn 115 120 125 Phe Gly Glu Ile Ala Phe Asp Gly Met Phe Gly Val Ala Pro Asn Tyr 130 135 140 Arg Val Phe Thr Asp Tyr Ala Glu Ser Val Glu Val Leu Lys Gly Pro 145 150 155 160 Thr Ser Phe Leu Tyr Gly Ile Ser Pro Asn Gly Gly Val Gly Gly Thr 165 170 175 Ile Asn Ile Val Pro Lys Arg Ala Leu Asp Thr Asp Leu Thr Arg Val 180 185 190 Thr Thr Ser Tyr Glu Ser Asp Leu Gln Val Gly Thr His Val Asp Ile 195 200 205 Ser Arg Arg Tyr Gly Ser Glu Arg Gln Phe Gly Val Arg Leu Asn Gly 210 215 220 Ser Val Gln Gly Gly Asp Gly Thr Ile Asp Asp Leu Ser Arg Phe Ala 225 230 235 240 Tyr Val Gly Ala Val Ala Leu Asp Tyr Glu Gly Glu Asn Leu Arg Ala 245 250 255 Thr Leu Asp Val Ile Asn Gln Tyr Glu His Tyr Asp Ala Pro Gln Arg 260 265 270 Pro Phe Tyr Pro Thr Ala Gly Ile Lys Leu Pro Ser Ala Pro Asp Asn 275 280 285 Arg Leu Asn Val Gln Glu Ser Trp Glu Trp Ser Ala Thr Arg Glu Phe 290 295 300 Ser Thr Leu Gly Arg Val Glu Tyr Asp Leu Ser Asp Asp Val Thr Val 305 310 315 320 Phe Gly Ala Val Gly Gly Gly Lys Ser Asn Val Glu Arg Leu Phe Gly 325 330 335 Thr Pro Thr Ile Thr Asp Ser Ala Gly Asn Val Ser Ile Val Pro Gln 340 345 350 His Tyr Ile Phe Asp Val Gln Arg Arg Thr Ala Glu Ile Gly Thr Arg 355 360 365 Gly Gln Phe Asp Thr Gly Ile Ile Glu His Ser Val Thr Leu Gln Ala 370 375 380 Asn Tyr Met Gln Trp Leu Ser Arg Gly Ser Asn Ser Gly Thr Ala Gln 385 390 395 400 Thr Thr Asn Leu Tyr Asn Pro Val Asp Arg Glu Glu Gln Phe Val Ala 405 410 415 Lys Pro Ser Ser Val Pro Lys Val Thr Glu Ser Glu Phe Tyr Gly Val 420 425 430 Ala Leu Ser Asp Thr Met Ser Ile Trp Asp Glu Arg Ala Gln Leu Met 435 440 445 Val Gly Gly Arg Phe Gln His Ile Asp Ser Glu Asn Tyr Ser Ser Thr 450 455 460 Thr Gly Ala Val Thr Ser Ser Ser Asp Ala Ser Ala Ile Thr Pro Met 465 470 475 480 Val Gly Val Val Val Lys Pro Trp Glu Asn Val Ser Leu Tyr Ala Asn 485 490 495 Tyr Ala Glu Gly Leu Ser Ile Gly Glu Thr Ala Pro Thr Asn Ala Val 500 505 510 Asn Ala Gly Glu Thr Leu Ser Pro Tyr Lys Ser Lys Gln Tyr Glu Val 515 520 525 Gly Thr Lys Ile Asp Thr Gly Pro Val Thr Leu Thr Ala Ser Leu Phe 530 535 540 Gln Ile Glu Lys Pro Phe Gly Val Leu Glu Thr Arg Gly Ser Asp Leu 545 550 555 560 Val Phe Glu Arg Gly Gly Glu Gln Arg Asn Arg Gly Leu Glu Leu Ser 565 570 575 Ala Phe Gly Glu Leu Thr Asp Thr Val Arg Leu Leu Gly Gly Val Thr 580 585 590 Phe Met Arg Gly Glu Leu Thr Lys Thr Asn Asn Ala Ser Thr Gln Gly 595 600 605 Asn Asp Pro Ile Gly Val Pro Lys Val Leu Val Asn Leu Gly Ala Glu 610 615 620 Trp Asp Thr Pro Phe Leu Ser Gly Phe Thr Leu Thr Gly Asn Val Ile 625 630 635 640 His Thr Gly Lys Gln Tyr Ala Asp Thr Ala Asn Ile Gln Lys Leu Pro 645 650 655 Ala Trp Thr Arg Leu Asp Leu Gly Ala Arg Tyr Lys Thr Thr Ile Lys 660 665 670 Glu Arg Pro Val Thr Phe Arg Ala Glu Val Glu Asn Val Phe Asn Lys 675 680 685 Asn Tyr Trp Ser Gly Val Ala Ser Phe Gly Thr Val Thr Gln Gly Ala 690 695 700 Pro Leu Thr Val Lys Ile Ser Met Thr Thr Asp Phe 705 710 715 34717DNAA. vitis strain F2/5gene(1)..(717)P1397 34atgaccagat ccagccgtga tcccctttat cgtcgccacc gatttcccgc cgaggtgatt 60gcccatgcag tttggctgta tttccggttt ccgcttagcc tgcggatggt cgaggatctg 120ctggcagcgc gtggcatcat cgtctctcat caaagcgtgc ggctctgggc ggagaaattc 180ggtagacatt ttgccaacga tatccgcaag cgatctaccg gcaggctcgg ggacaaatgg 240catctcgatg aggttgtcat caccatcggt ggaaggaaac actggctttg gcgcgccgtt 300gatcaggatg gctttgttct cgatgtgctg gtgcaaaacc gcagcaatac caaggcggca 360aaacgtttga tgcgcaagct cctgaaagcc caaggccgtg cgctgcgtgt gatgattacc 420gacaaactac gatcctatgg tgcagcaaag cgggaggtca tgcccggcat tgagcatcgt 480tctcacaaag gattgaaaaa tcggtcggaa aactctcatc agcccgtccg acgacgggag 540aggatcatga agcgcttcaa gtcaacacga catcttcagc gtttcgtttc catccatgac 600ccgatcacca acctttttca cattccccgc cacgatattc catccgtcca ccatcgcgaa 660ctgcgaacag ctgccatgca agcatggcac caaatcgcgc gccttcacgc cgcatga 71735238PRTA. vitis strain F2/5 35Met Thr Arg Ser Ser Arg Asp Pro Leu Tyr Arg Arg His Arg Phe Pro 1 5 10 15 Ala Glu Val Ile Ala His Ala Val Trp Leu Tyr Phe Arg Phe Pro Leu 20 25 30 Ser Leu Arg Met Val Glu Asp Leu Leu Ala Ala Arg Gly Ile Ile Val 35 40 45 Ser His Gln Ser Val Arg Leu Trp Ala Glu Lys Phe Gly Arg His Phe 50 55 60 Ala Asn Asp Ile Arg Lys Arg Ser Thr Gly Arg Leu Gly Asp Lys Trp 65 70 75 80 His Leu Asp Glu Val Val Ile Thr Ile Gly Gly Arg Lys His Trp Leu 85 90 95 Trp Arg Ala Val Asp Gln Asp Gly Phe Val Leu Asp Val Leu Val Gln 100 105 110 Asn Arg Ser Asn Thr Lys Ala Ala Lys Arg Leu Met Arg Lys Leu Leu 115 120 125 Lys Ala Gln Gly Arg Ala Leu Arg Val Met Ile Thr Asp Lys Leu Arg 130 135 140 Ser Tyr Gly Ala Ala Lys Arg Glu Val Met Pro Gly Ile Glu His Arg 145 150 155 160 Ser His Lys Gly Leu Lys Asn Arg Ser Glu Asn Ser His Gln Pro Val 165 170 175 Arg Arg Arg Glu Arg Ile Met Lys Arg Phe Lys Ser Thr Arg His Leu 180 185 190 Gln Arg Phe Val Ser Ile His Asp Pro Ile Thr Asn Leu Phe His Ile 195 200 205 Pro Arg His Asp Ile Pro Ser Val His His Arg Glu Leu Arg Thr Ala 210 215 220 Ala Met Gln Ala Trp His Gln Ile Ala Arg Leu His Ala Ala 225 230 235 363879DNAA. vitis strain F2/5misc_feature(1)..(3879)avi5730 36ttgtcgcagg cgggccatga agtggtctat gggcgtcagt ctatcggctt tcgcttcgcg 60cctgtcgggg ttgatcgtgc acacgctgga attgagacgc tgatgcttcg tcatcccctg 120ttaaatcgtc gctttgaagt gcgggcaggc gggatcgttt atcagttgct tggccaaaag 180cccttgcctg ttgttgagcg tgagcttgca aaggatgggg agttggagac tgtattacgg 240gaggtttcct ccacctattc cggtagggcg cttgatctgg aaaaggatgc cgccgcgcaa 300tttacgctat ttacggtagg tgggcaggct attggcctat tgttgtcgtt gcatcctctg 360attggtgacc ggacggccct cgaccggttg gccgtcgatt tccttgatgg gcttgatcga 420caggctgatt tagggcgtga agaggcgggt ttgcttgatg cagcacattg gctggttcaa 480ggcgccgcaa tgccgcagat agcagatgct gacgccgatt tctggtttaa ccgattggtg 540gcacaggaga cggtggcgat gctgcccgcc caggctccag cggcaggtgt ggcaaattct 600ggtccgcaaa catggcgcga gcaggtcatc gaggtcgtgt gctcggcctc gacatctgtg 660gttgatgtga cgcaggacag tctggcggcg ctggccattg tgcttcgtcg ctatagcggt 720tgtggtaccc agcggatcgg gctggtgacg cggcaggccg attgccccgt tgcgacccgc 780accgaagatc tgttgctcgt caccagcgaa atcgacggtc gtttggctct tgacgcagcg 840cgcgaacggc tgggcgaggg ggttgccacc gcgctgtccc accatttgcc gttcgaagcg 900ctgacgaatg ccctggcgcg gcgcgacgat gggttcgagg cagccagcct ggttgcaacg 960gtgctggatg tgcggcccgc cctccagctg gaggctcaag gtgtggccgg tcagccggtt 1020gccgttctgg tagagccgcc tgcgcggcgc gatgcaggtc tggtggttac cgtttccggt 1080ctggggtcgg agacattggc gatccggctg ggctatgacc agcagagcca tagtgatgca 1140atgatcgacc ggttcgccca cgatctccgt ctggcgttcg aggctctgcg ggggcagccg 1200gagcagctgg tcagagcgat tgctttcatg tcggttgaag agctggaccg gctgtcagcg 1260ccctatcccg accagcctga gaccgatgat ggcacgccga tccatcaggt gatttcggcc 1320caggcgcagc gaaggccaga cgctttcgcg gtggcgcagg gcgatacgtc aattacccat 1380ggcgcgctgg aagccgccgc caaccggctc gcccatcggc tggtcgccat ggggatcggc 1440ccggaagacc gagtcgccgt ggcgctgaac aaatccatcg acgcgattat cgccattctg 1500gcggtgctga aggccggtgg cgcttttacg ccggtcgagc cggatcatcc agaagcccgc 1560aaccgccata tcctgagcgc gccgggtctg acgctggtga tttcgcgggg gcgttatatc 1620accgatctgc cgcgtgatat cggcacgccg atcctcaatc tcgacacgct cgatctatcc 1680tcggaaagca ccgagccgcc ggtcatcgcc attgcgccag cccagcttgc ctatgtgatc 1740tatacgtccg gctccaccgg tatccccaag ggggtggctg ttgagcatgg tccgctggcg 1800catcattgca aggcgacgct acgcatctat gaaatggatg agacttcctg cgaatatccg 1860gtcttgccct tcacctcgga tggtgggcat gaacgctgga tggtgccgct gatggcgggt 1920ggcggtgtgg tgctgacggc ggataagctg gcgacgccgg aagatgcttt cgcgttgatg 1980cgcaggcacg gcgtcaacaa tgccagcttg ccgaccagct atgtccgggg ccttgccgaa 2040tatgcggcgg aaaagggcgg gataccgcag ctgcggctct attccttcgg cggtgaagcg 2100ctatcccagg cggtcttcga tctgctgacc gataatctca aggcgcagat gctgatcaac 2160ggttatgggc caaccgaaac catcatgacg ccgatggtgt ggaaaatccc ggcgggaacc 2220cggtttgagg gaacggttgc accgattggc cggggtgtag gcgaccgccg catctatgtg 2280ctggacagcg atctggtgcc ggtgccggtc ggggtgatcg gcgagatcca tatcggcggc 2340agcggtattg ctcgcggcta tctcggccag ccggagctga cggcggatcg tttcattgat 2400gatccttttt ccgccaacgg tgggcggatg tacaagtccg gcgatctcgg ccgctggcgc 2460gaagatggca ctgtagaatt tgccggccgg gtcgatcacc agatcaagtt gcgtggctat 2520cgcatcgagc cgggcgaaat cgaggcggtg ctgcgggccg atccgaacgt gtcggaagcc 2580gtggtgctgc tgcatcagga cagtggacgc agcgcgttga tcgcctatgt ggtggcgcgt 2640gacgatgagg atgtcaacgt caacgatctt cgccgcgccg ccgtcaccgc cctgccggat 2700tacatggtgc cgcagcatat catggtgctt gatgcgctgc cgatggggcc gaacagcaag 2760cttgatcgaa gcgcgctgcc gctgcccaag ctgcaacgtg atatcgtccc ccccgccgac 2820gacaaggaag ccgccattct ggaggtctgg aaacaggtcc ttgatatcgc agaactcagc 2880gtcaccgaaa acttcttcga tgtcggcggc cagtcgctgg cggcggtgcg gatcgtctcg 2940cggctgaaaa tgcagcaccc gaaatggccg ctgaccattg ccgatatgtt caactatccg 3000accgtgcggg atctggcgct ggcgatggac gaaaaccggc aggaggacaa ggtcggggcg 3060atctatctgc gccgcgacgg cgaccgtccg gtgctctatt gcttcccggg tcttctggtc 3120agcacgcggg aatatatgcg gttggtggat tatctcgggc cgaaccagcc ggccacgggc 3180ttcgtctgct attcgctgac cgaaacgcca gcgctcagca ccagggtcga ggatattacc 3240gcccgctacg ccgaagcggt gcgcagccag gctaagggac ggccttgcgc ctttcttggg 3300tggtcctggg gcgggcttct cgcctatgag gcagcgcagc agcttggcaa tgacgtcgat 3360cttcggatga tcggcatggt cgatgtctgc gacatgggcg atgaatttgc cattggcgtc 3420tggccgcatt tcgaacctgg cgtgcgcgaa cgaacccatg aggccgtgca gcgctggctg 3480acagtcgcgc caatgcgcga ggcctggttg acgctaatgg cggcgatgga tgccgaggtt 3540tacgagcaat tcctgcatca tatcgttaag cataacgtgc cgctgcctgt cgatgggccg 3600gatatcggct cggaagagca tattttctgg gttctgctcg ataacgcgat gattttccgt 3660aattatcagc tgaaaacctc cgcgttccgc atccatgcgt tttcggctga agattcggta 3720acgcggggcc tcagtgtcat cgactggcgc cgctattctc ctaatgcgac ggcttgcgaa 3780ttggtgaccg gcaccaacca tctgtcgatt atcggcaagt cccgtttcca tcagcgtttt 3840gcccagcggc tcgatctagc catccaggac aagccctaa 3879371292PRTA. vitis strain F2/5MISC_FEATURE(1)..(1292)avi5730 37Met Ser Gln Ala Gly His Glu Val Val Tyr Gly Arg Gln Ser Ile Gly 1 5 10 15 Phe Arg Phe Ala Pro Val Gly Val Asp Arg Ala His Ala Gly Ile Glu 20 25 30 Thr Leu Met Leu Arg His Pro Leu Leu Asn Arg Arg Phe Glu Val Arg 35 40 45 Ala Gly Gly Ile Val Tyr Gln Leu Leu Gly Gln Lys Pro Leu Pro Val 50 55 60 Val Glu Arg Glu Leu Ala Lys Asp Gly Glu Leu Glu Thr Val Leu Arg 65 70 75 80 Glu Val Ser Ser Thr Tyr Ser Gly Arg Ala Leu Asp Leu Glu Lys Asp 85 90 95 Ala Ala Ala Gln Phe Thr Leu Phe Thr Val Gly Gly Gln Ala Ile Gly 100 105 110 Leu Leu Leu Ser Leu His Pro Leu Ile Gly Asp Arg Thr Ala Leu Asp 115 120 125 Arg Leu Ala Val Asp Phe Leu

Asp Gly Leu Asp Arg Gln Ala Asp Leu 130 135 140 Gly Arg Glu Glu Ala Gly Leu Leu Asp Ala Ala His Trp Leu Val Gln 145 150 155 160 Gly Ala Ala Met Pro Gln Ile Ala Asp Ala Asp Ala Asp Phe Trp Phe 165 170 175 Asn Arg Leu Val Ala Gln Glu Thr Val Ala Met Leu Pro Ala Gln Ala 180 185 190 Pro Ala Ala Gly Val Ala Asn Ser Gly Pro Gln Thr Trp Arg Glu Gln 195 200 205 Val Ile Glu Val Val Cys Ser Ala Ser Thr Ser Val Val Asp Val Thr 210 215 220 Gln Asp Ser Leu Ala Ala Leu Ala Ile Val Leu Arg Arg Tyr Ser Gly 225 230 235 240 Cys Gly Thr Gln Arg Ile Gly Leu Val Thr Arg Gln Ala Asp Cys Pro 245 250 255 Val Ala Thr Arg Thr Glu Asp Leu Leu Leu Val Thr Ser Glu Ile Asp 260 265 270 Gly Arg Leu Ala Leu Asp Ala Ala Arg Glu Arg Leu Gly Glu Gly Val 275 280 285 Ala Thr Ala Leu Ser His His Leu Pro Phe Glu Ala Leu Thr Asn Ala 290 295 300 Leu Ala Arg Arg Asp Asp Gly Phe Glu Ala Ala Ser Leu Val Ala Thr 305 310 315 320 Val Leu Asp Val Arg Pro Ala Leu Gln Leu Glu Ala Gln Gly Val Ala 325 330 335 Gly Gln Pro Val Ala Val Leu Val Glu Pro Pro Ala Arg Arg Asp Ala 340 345 350 Gly Leu Val Val Thr Val Ser Gly Leu Gly Ser Glu Thr Leu Ala Ile 355 360 365 Arg Leu Gly Tyr Asp Gln Gln Ser His Ser Asp Ala Met Ile Asp Arg 370 375 380 Phe Ala His Asp Leu Arg Leu Ala Phe Glu Ala Leu Arg Gly Gln Pro 385 390 395 400 Glu Gln Leu Val Arg Ala Ile Ala Phe Met Ser Val Glu Glu Leu Asp 405 410 415 Arg Leu Ser Ala Pro Tyr Pro Asp Gln Pro Glu Thr Asp Asp Gly Thr 420 425 430 Pro Ile His Gln Val Ile Ser Ala Gln Ala Gln Arg Arg Pro Asp Ala 435 440 445 Phe Ala Val Ala Gln Gly Asp Thr Ser Ile Thr His Gly Ala Leu Glu 450 455 460 Ala Ala Ala Asn Arg Leu Ala His Arg Leu Val Ala Met Gly Ile Gly 465 470 475 480 Pro Glu Asp Arg Val Ala Val Ala Leu Asn Lys Ser Ile Asp Ala Ile 485 490 495 Ile Ala Ile Leu Ala Val Leu Lys Ala Gly Gly Ala Phe Thr Pro Val 500 505 510 Glu Pro Asp His Pro Glu Ala Arg Asn Arg His Ile Leu Ser Ala Pro 515 520 525 Gly Leu Thr Leu Val Ile Ser Arg Gly Arg Tyr Ile Thr Asp Leu Pro 530 535 540 Arg Asp Ile Gly Thr Pro Ile Leu Asn Leu Asp Thr Leu Asp Leu Ser 545 550 555 560 Ser Glu Ser Thr Glu Pro Pro Val Ile Ala Ile Ala Pro Ala Gln Leu 565 570 575 Ala Tyr Val Ile Tyr Thr Ser Gly Ser Thr Gly Ile Pro Lys Gly Val 580 585 590 Ala Val Glu His Gly Pro Leu Ala His His Cys Lys Ala Thr Leu Arg 595 600 605 Ile Tyr Glu Met Asp Glu Thr Ser Cys Glu Tyr Pro Val Leu Pro Phe 610 615 620 Thr Ser Asp Gly Gly His Glu Arg Trp Met Val Pro Leu Met Ala Gly 625 630 635 640 Gly Gly Val Val Leu Thr Ala Asp Lys Leu Ala Thr Pro Glu Asp Ala 645 650 655 Phe Ala Leu Met Arg Arg His Gly Val Asn Asn Ala Ser Leu Pro Thr 660 665 670 Ser Tyr Val Arg Gly Leu Ala Glu Tyr Ala Ala Glu Lys Gly Gly Ile 675 680 685 Pro Gln Leu Arg Leu Tyr Ser Phe Gly Gly Glu Ala Leu Ser Gln Ala 690 695 700 Val Phe Asp Leu Leu Thr Asp Asn Leu Lys Ala Gln Met Leu Ile Asn 705 710 715 720 Gly Tyr Gly Pro Thr Glu Thr Ile Met Thr Pro Met Val Trp Lys Ile 725 730 735 Pro Ala Gly Thr Arg Phe Glu Gly Thr Val Ala Pro Ile Gly Arg Gly 740 745 750 Val Gly Asp Arg Arg Ile Tyr Val Leu Asp Ser Asp Leu Val Pro Val 755 760 765 Pro Val Gly Val Ile Gly Glu Ile His Ile Gly Gly Ser Gly Ile Ala 770 775 780 Arg Gly Tyr Leu Gly Gln Pro Glu Leu Thr Ala Asp Arg Phe Ile Asp 785 790 795 800 Asp Pro Phe Ser Ala Asn Gly Gly Arg Met Tyr Lys Ser Gly Asp Leu 805 810 815 Gly Arg Trp Arg Glu Asp Gly Thr Val Glu Phe Ala Gly Arg Val Asp 820 825 830 His Gln Ile Lys Leu Arg Gly Tyr Arg Ile Glu Pro Gly Glu Ile Glu 835 840 845 Ala Val Leu Arg Ala Asp Pro Asn Val Ser Glu Ala Val Val Leu Leu 850 855 860 His Gln Asp Ser Gly Arg Ser Ala Leu Ile Ala Tyr Val Val Ala Arg 865 870 875 880 Asp Asp Glu Asp Val Asn Val Asn Asp Leu Arg Arg Ala Ala Val Thr 885 890 895 Ala Leu Pro Asp Tyr Met Val Pro Gln His Ile Met Val Leu Asp Ala 900 905 910 Leu Pro Met Gly Pro Asn Ser Lys Leu Asp Arg Ser Ala Leu Pro Leu 915 920 925 Pro Lys Leu Gln Arg Asp Ile Val Pro Pro Ala Asp Asp Lys Glu Ala 930 935 940 Ala Ile Leu Glu Val Trp Lys Gln Val Leu Asp Ile Ala Glu Leu Ser 945 950 955 960 Val Thr Glu Asn Phe Phe Asp Val Gly Gly Gln Ser Leu Ala Ala Val 965 970 975 Arg Ile Val Ser Arg Leu Lys Met Gln His Pro Lys Trp Pro Leu Thr 980 985 990 Ile Ala Asp Met Phe Asn Tyr Pro Thr Val Arg Asp Leu Ala Leu Ala 995 1000 1005 Met Asp Glu Asn Arg Gln Glu Asp Lys Val Gly Ala Ile Tyr Leu 1010 1015 1020 Arg Arg Asp Gly Asp Arg Pro Val Leu Tyr Cys Phe Pro Gly Leu 1025 1030 1035 Leu Val Ser Thr Arg Glu Tyr Met Arg Leu Val Asp Tyr Leu Gly 1040 1045 1050 Pro Asn Gln Pro Ala Thr Gly Phe Val Cys Tyr Ser Leu Thr Glu 1055 1060 1065 Thr Pro Ala Leu Ser Thr Arg Val Glu Asp Ile Thr Ala Arg Tyr 1070 1075 1080 Ala Glu Ala Val Arg Ser Gln Ala Lys Gly Arg Pro Cys Ala Phe 1085 1090 1095 Leu Gly Trp Ser Trp Gly Gly Leu Leu Ala Tyr Glu Ala Ala Gln 1100 1105 1110 Gln Leu Gly Asn Asp Val Asp Leu Arg Met Ile Gly Met Val Asp 1115 1120 1125 Val Cys Asp Met Gly Asp Glu Phe Ala Ile Gly Val Trp Pro His 1130 1135 1140 Phe Glu Pro Gly Val Arg Glu Arg Thr His Glu Ala Val Gln Arg 1145 1150 1155 Trp Leu Thr Val Ala Pro Met Arg Glu Ala Trp Leu Thr Leu Met 1160 1165 1170 Ala Ala Met Asp Ala Glu Val Tyr Glu Gln Phe Leu His His Ile 1175 1180 1185 Val Lys His Asn Val Pro Leu Pro Val Asp Gly Pro Asp Ile Gly 1190 1195 1200 Ser Glu Glu His Ile Phe Trp Val Leu Leu Asp Asn Ala Met Ile 1205 1210 1215 Phe Arg Asn Tyr Gln Leu Lys Thr Ser Ala Phe Arg Ile His Ala 1220 1225 1230 Phe Ser Ala Glu Asp Ser Val Thr Arg Gly Leu Ser Val Ile Asp 1235 1240 1245 Trp Arg Arg Tyr Ser Pro Asn Ala Thr Ala Cys Glu Leu Val Thr 1250 1255 1260 Gly Thr Asn His Leu Ser Ile Ile Gly Lys Ser Arg Phe His Gln 1265 1270 1275 Arg Phe Ala Gln Arg Leu Asp Leu Ala Ile Gln Asp Lys Pro 1280 1285 1290