Regulation of respiration in rotenone-treated tobacco cell suspension cultures.
关键词
抽象
Cells of Nicotiana tabacum L. suspension cultures were treated with the respiratory inhibitor rotenone, which specifically inhibits complex I activity of mitochondria. Rotenone retarded cell growth, as shown by decreases in fresh weight, dry weight and cell numbers on a suspension-volume basis. However, rates of the coupled respiration were higher in rotenone-treated compared to control cells when expressed on a fresh-weight basis. Rates of the rotenone-insensitive respiration increased substantially on both a fresh-weight and extractable-cellular-protein basis 24 h after rotenone treatment. ATP/ADP ratios were not significantly different between control and rotenone-treated cells. Our results indicated that cells of tobacco suspension cultures were able to maintain a slow rate of growth and adequate ATP/ADP ratios without the operation of complex I.