[Study on enzyme extraction technology of total flavonoids from Cryptotaenia japonica].
关键词
抽象
OBJECTIVE
To study the optimum enzyme extraction process of total flavonoids from Cryptotaenia japonica.
METHODS
The process was optimized by single factor experiments and orthogonal, the yield of total flavonoids was used as index.
RESULTS
The optimum extraction technology was as follows: enzyme dosage: 1U/mL, pH value: 6.0, enzymatic hydrolysis temperature: 60%, enzymatic hydrolysis time: 1 h, the rate of material to liquid: 1 : 30, extraction temperature: 70 degrees C, extraction time: 1 h, the average extraction rate of total flavonoids from Cryptotaenia japonica was 4.76%.
CONCLUSIONS
The method is an effective way to extract total flavonoids from Cryptotaenia japonica with high extract rate.