Two new abietane diterpenes huperphlegmarins A and B from Huperzia phlegmaria.

Two new abietane diterpenes, huperphlegmarin A-B (1-2), were isolated from the aerial parts of Huperzia phlegmaria (L.) Rothm. (Lycopodiaceae), in addition to five known compounds including lycoxanthol (3), 21β-hydroxyserrat-14-en-3β-yl acetate (4), 21α-hydroxyserrat-14-en-3β-yl acetate (5), 21α-hydroxyserrat-14-en-3β-ol (6), and fawcettidine (7). Their structures were determined by the combination analyses of spectroscopy, including 1D-, 2D-NMR, HRESIMS, CD and comparison with the reported data in the literature. Huperphlegmarin B (2) presented the rare 1,11-epoxy group in the molecule. Compounds 3, 4, 5 and 6 were performed for AChE inhibitory activity and compound 3 showed the inhibitory activity against AChE with IC50 of 465.6 μg/mL.