Spirometra species from Asia: Genetic diversity and taxonomic challenges

Despite considerable controversy concerning the taxonomy of species within the genus Spirometra, human sparganosis and spirometrosis mainly in Asia and Europe has long been confidently ascribed to Spirometra erinaceieuropaei. Recently, the mitochondrial genomes of purported "S. erinaceieuropaei", "Spirometra decipiens" and "Spirometra ranarum" from Asia have been determined. However, it has been pointed out that the morphological criteria used for identifying these species are unsuitable and thus these identifications are questionable. In the present study, therefore, Spirometra samples from Asia were re-examined based on mitochondrial cytochrome c oxidase subunit 1 gene sequences and the identification of these species was discussed. Haplotype network and phylogenetic analyses revealed that: i) two distinct Spirometra species, Type I and Type II, are present in Asia and neither of which is close to likely European "S. erinaceieuropaei"; ii) Type I is genetically diverse and widely distributed, however Type II is known so far from Japan and Korea; iii) Type I is probably conspecific with Spirometra mansoni, and Type II may represent an undescribed species; iv) "S. decipiens" and "S. ranarum" reported from Asia are conspecific with Type I. We propose that Type I is most probably S. mansoni and Type II is likely an undescribed species.

Keywords: Asia; Genetic diversity; Human sparganosis; Mitochondrial cytochrome c oxidase subunit 1 gene; Spirometra mansoni; Spirometra species.