4 结果
Callus cultures of Choisya ternata have been prepared by different strategies: aggregate clones, subclones and protoclones obtained from well-established strains; protoclones obtained from mesophyll tissue; cultures transformed by Agrobacterium tumefaciens. All of them show high variability in their
The accumulation of two dihydrofuroquinoline alkaloids has been studied in seventeen strains of Choisya ternata (Rutaceae) initiated from the same tree. The contents of platydesminium and balfourodinium vary in heavy proportions. The effect of some factors (age of the culture, NAA or glucose
Protoclones and aggregate clones have been prepared from 5 callus strains of C. ternata chosen for their dihydrofuroquinoline-accumulating capacities in a population of well-established strains. The results show that it is possible to obtain cell lines which accumulate higher alkaloid contents than
The absorption and fluorescence properties of six furoquinoline or pyranoquinoline alkaloids extracted from Rutaceae plants are described. These properties are related to the molecular structure and used for the fluorometric determination of two of them (platydesminium and balfourodinium) in Choisya