A rapid, sensitive, and validated method was developed for the simultaneous determination of four iminosugars, 1-deoxynojirimycin (DNJ), d-fagomine (FAG), 2-O-a-d-galactopyranosyl-DNJ (Gal-DNJ), and 4-O-β-d-glucopyranosyl-fagomine (Glu-FAG), in mulberry leaves. The method used hydrophilic