3 结果
Unopened flower heads of Haplopappus gracilis (2n = 4) provided primary explants for callus production and subsequent induction of organized growth. Callus was initiated from small (3-5 mm in length) floral buds with benzylaminopurine (BAP) (44.4 micromoles; 10 mg/l) and naphthalene acetic acid
Suspension cultures of Haplopappus gracilis accumulated anthocyanin when grown in defined media with 4.5×10(-6)M 2,4-D. Transfer of cells to media with 10(-5)M kinetin or benzyladenine and no auxin or 10(-7)M NAA for 6 days resulted in increased anthocyanin concentration of the cells but the total
Suspensions of Haplopappus gracilis cells, containing about 80% free cells, were obt ained from log-phase cultures by filtration through 3 nylon sieves having decreasing mesh widths from 297, 210 and 88 μm. From the free cell suspensions, 75 to 90% of the cells developed into visible colonies when