isomaltose/hyperglycemia

A simple method of preparing a chitin-immobilized alpha-amylase and glucoamylase from the protease- and glycosidase-less Mutant HF-15 of Aspergillus awamori var. kawachi was developed and used for the production of high-glucose syrup. The glucoamylase was tightly bound onto chitin without the aid of

Glucoamylase mutations to reduce isomaltose formation from glucose condensation and thus increase glucose yield from starch hydrolysis were designed to produce minor changes in the active site at positions not totally conserved. Tyr175-->Phe and Ser411-->Gly glucoamylases had catalytic efficiencies

Mutations Asn20-->Cys/Ala27-->Cys (SS), Ala27-->Pro, Ser30-->Pro, Lys108-->Arg, Gly137-->Ala, Tyr312-->Trp and Ser436-->Pro in Aspergillus awamori glucoamylase, along with a mutation inserting a seven-residue loop between Tyr311 and Gly314 (311-314 Loop), were made to increase glucose yield from