phlorizin/hypoxia

Hypoxia is a common denominator of many vascular disorders, especially those associated with ischemia. To study the effect of oxygen depletion on endothelium, we developed an in vitro model of hypoxia on human umbilical vein endothelial cells (HUVEC). Hypoxia strongly activates HUVEC, which then

Lactase-phlorizin hydrolase, a brush-border membrane disaccharidase, is a marker of intestinal epithelial cell differentiation and digestive function. The intestine is susceptible to conditions of hypoxia resulting from vascular perfusion deficits. We hypothesized that lactase gene induction may

Early stage diabetic nephropathy is characterized by glomerular hyperfiltration and reduced renal tissue Po2. Recent observations have indicated that increased tubular Na(+)-glucose linked transport (SGLT) plays a role in the development of diabetes-induced hyperfiltration. The aim of the present

The transepithelial transport of ascorbate across the isolated rabbit ciliary epithelium (CE) was investigated. Unidirectional 14C-ascorbate fluxes were measured in the presence of equal concentrations of ascorbate on both sides of the tissue within the range of 0.025 to 1 mM. The blood to aqueous

Water movements have been studied in sheets of isolated rabbit ileum using a method which measures net volume flows across the mucosal and serosal surfaces of the tissue continuously with high resolution. At 35 degrees C, with the tissues incubated in isotonic Ringer solution containing D-glucose

Mediated (nonactive) transport of glucose in mammalian cells is characterized by saturation kinetics, stereospecificity, sensitivity to inhibition by phlorizin and certain sulfhydryl-blocking agents, a temperature coefficient of about 2, an inability to utilize metabolic energy, and

Beneficial effect of glucose and insulin on the myocardium are still a matter of discussion. The influence of insulin on isometric force of contraction of right ventricular papillary muscles of guinea pigs was studied. The papillary muscles were mounted vertically in a 95% O2, 5% CO2 modified

Glucose transport into muscle cells occurs through facilitated diffusion mediated primarily by the GLUT1 and GLUT4 glucose transporters. These transporter proteins are controlled by acute and chronic exposure to insulin, glucose, muscle contraction, and hypoxia. We propose that acute responses occur

The evidence suggests that glucose transport across the blood brain barrier (BBB) in the dog is normally not a rate-limiting step in cerebral metabolism; however, transport may become rate-limiting under conditions of extreme hypoglycemia or anoxia. Studies on the mechanism of glucose transport from

The transport of glucose, other monosaccharides and disaccharides in the rat small intestine has been studied under the condition of a predominance of serosal-to-mucosal (SM) flows over mucosal-to-serosal (MS) ones. A dependence has been found of the efflux of glucose into the serosal solution on

Adipose tissue (AT) expansion induces local hypoxia, a key contributor to the chronic low-grade inflammation that drives obesity-associated disease. Apple flavonols phloretin (PT) and phlorizin (PZ) are suggested anti-inflammatory molecules but their effectiveness in obese AT is inadequately

Uptake of glucose-(3)H into cultured HLM cells was measured. Equilibration of intracellular and extracellular pools occurred after 25 min. Glucose influx was determined subsequently by measuring the glucose-(3)H entering in precisely 1 min. Although saturation kinetics were demonstrated these were

1. The effect of various monosaccharides on the potential difference across the intestine of the invertebrate, Cryptochiton stelleri, was studied using an everted sac technique.2. D-Glucose, when present in the mucosal solution, increased the transmural potential across the anterior intestine but

Using a new, in vitro, experimental approach the transport of glucose, galactose, fructose and glycine in seroso-mucosal direction in the small intestine, was studied under different modes of oxygenation. In case of monosaccharides under the oxygenation from serosal surface, the substrate