Kinetics of glucose transport in K-562 cells was studied using 3-O-methylglucose, a nonmetabolizable analog of glucose. A Km of 3.7 mM and Vmax of 32.0 nmoles/minute/10(6) cells was found for the process. D-Glucose, phloretin, and phlorizin competitively inhibit the transport of 3-O-methylglucose