One new secoiridoid glycoside with conjugated diene, named 3-epi-swertiajaposide C (1), was isolated from the whole plants of Gentiana apiata N.E. Br., together with 11 known compounds, 7-deoxyloganic acid (2), isoorientin (3), gentiopicroside (4), silybin B (5), swertiamarin (6), asystasioside A