solanum clarum/nicotine

Efficient plastid transformation has been achieved in Nicotiana tabacum using cloned plastid DNA of Solanum nigrum carrying mutations conferring spectinomycin and streptomycin resistance. The use of the incompletely homologous (homeologous) Solanum plastid DNA as donor resulted in a Nicotiana

Gametophytic self-incompatibility (GSI) systems involving the expression of stylar ribonucleases have been described and extensively studied in many plant families including the Solanaceae, Rosaceae and Scrophulariaceae. Pollen recognition and rejection is governed in the style by specific

Wild species often show more tolerance to environmental stress factors than their cultivated counterparts. An early responsive-to-dehydration gene was cloned from a drought- and salt-tolerant wild tomato Solanum pennellii (SpERD15). SpERD15 transcript accumulated differentially in different organs,

The conformational transitions of viroid-like RNAs associated with cadang-cadang disease, velvet tobacco mottle virus, and solanum nodiflorum mottle virus were studied by melting analysis and fast temperature jump technique in 1 mM sodium-cacodylate, 10 mM NaCl, 0.1 mM EDTA, pH 6.8. The 4 circular

Velvet tobacco mottle virus (VTMoV) isolated from Nicotiana velutina growing wild in arid Central Australia was transmitted by inoculation to a limited number of plant species of which N. clevelandii was the most convenient experimental host. The virus was also transmitted from field-grown plants

Velvet tobacco mottle virus (VTMoV) and Solanum nodiforum mottle virus (SNMV) are serologically closely related as shown by immunodiffusion tests. Dissociated VTMoV coat protein separates into three electrophoretic components in polyacrylamide gels corresponding to polypeptides with Mr's of about

Solanum nodiforum mottle virus (SNMV) encapsidates two unique RNA species as shown by 3H-complementary DNA hybridization analysis. They are RNA 1, a long linear single-stranded molecule with a molecular weight of 1.5 x 10(6) and RNA 2 and RNA 3 which are circular and linear forms of the same

Neither the virus-like RNA (RNA 1) nor the viroid-like RNA (RNA 2) of velvet tobacco mottle virus (VTMoV) is capable of independent replication in Nicotiana clevelandii. The function of RNA 2 for the RNA 1 replication could not be fulfilled by conventional viroids such as those of chrysanthemum

The effects of broadleaf tobacco, tomato, and black nightshade on juvenile hatch and reproduction of Globodera tabacum tabacum were determined in laboratory and greenhouse experiments. Root exudates from nightshade stimulated greater egg hatch than those from either 'Rutgers' tomato or '86-4'

Tobacco, eastern black nightshade, and tomato were grown for 3 to 13 weeks to assess differences in invasion, development, and soil density of Globodera tabacum tabacum (tobacco cyst nematode) in field plots and microplots over three seasons. Tobacco cyst nematodes invaded roots of resistant and

Oesophageal cancer is common in Africa, and is a major cause of death in Transkei, South Africa. The cause of this endemic disease has been debated for many decades. A continuing focus of research has been identification of a single potent carcinogen. I argue that endemic incidence of oesophageal

The effects of temperature and type of host on the evolution of variants in replicating populations of tobacco mosaic virus (TMV) originating from an in vitro RNA transcript of a cDNA clone were studied. The phenotypic markers measured were temperature-sensitive (ts) replication and induction of

Understanding the mechanism of abiotic-tolerance and producing germplasm of abiotic tolerance are important in plant research. Wild species often show more tolerance of environmental stress factors than their cultivated counterparts. Genes from wild species show potential abilities to improve

Salinity severely affects plant growth and development. Plants evolved various mechanisms to cope up stress both at molecular and cellular levels. Halophytes have developed better mechanism to alleviate the salt stress than glycophytes, and therefore, it is advantageous to study the role of

The purpose of this study was to develop a rapid analytical method for the reliable determination of low concentrations of nicotine in foods for large numbers of samples. Food material was extracted using a simple liquid-liquid extraction method. For processed foods, further clean-up steps had to be