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The purpose of the current study was to establish a valid protocol for nerve cryopreservation, and to evaluate if the addition of albumin supposed any advantage in the procedure. We compared a traditional cryopreservation method that uses dimethyl sulfoxide (DMSO) as cryoprotectant, to an
BACKGROUND
It has been shown that hyaluronan (HA) can decrease peritoneal fluid absorption. It is not known, however, how various molecular weights and various concentrations of hyaluronan affect peritoneal fluid absorption rate.
METHODS
A study of 4-hour dwells, with frequent dialysate and blood
Copper-62 labeled pyruvaldehyde bis (N4-methyl-thiosemicarbazonato) copper(II), CuPTSM, has been used to probe tissue perfusion by means of positron emission tomography. Despite promising results from animals, problems have been encountered in the use of 62CuPTSM to quantitate regional myocardial
Eighteen commercial bovine serum albumin (BSA) preparations were evaluated for their efficacy in hemagglutination enhancement by the capillary tube method. Their physicochemical variables (pH, conductivity, free fatty acids, total protein, and polymer content) were quantified. The results showed
The composition of the protein layer adsorbed to a polymer has been thought to be important for the adhesion of platelets. The state of activation of adherent platelets is an additional factor that may be a predictor of biocompatibility. Activation refers to the degree of change from discoid shape
A new type of chromatographic immunoassay based on sequential addition is described. On a protein A column, the antibody, the sample containing the antigen, and then a known amount of antigen are sequentially injected. This assay is designed to shorten analysis times and reduce complexity of
Effect of dyes of different classes on characteristics of X-chemoluminescence of human serum albumin solutions is studied. Intensification of lumininescence by the addition of uranin, eosin, tetrachlorfluorescein and erythrozan was found. The process of luminescence intensification with the addition
The serum protein, orosomucoid has been shown to be essential for the maintenance of normal capillary permeability in several different organs, including the kidney. Thus, the clearance of albumin was found to be almost fivefold higher in the absence of orosomucoid in a previous study on isolated
Electrically addressed covalent immobilization of biomolecules to the defined electrodes of an electrode array is described. It is based on Michael addition of the thiol group of biomolecules to α,β-unsaturated carbonyl groups of benzoquinone. This "click" reaction was tested by immobilization of a
A new chemical cross-linking scheme was utilized for the formation of degradable poly(ethylene glycol) hydrogels suitable for the delivery of protein drugs. An aqueous solution containing a PEG-multiacrylate and solid particles of albumin was mixed with an aqueous solution containing a PEG-dithiol,
The cryopreservation of dromedary camel (Camelus dromedarius) sperm has proved challenging with little success reported. The routine application of artificial insemination with frozen semen would assist the flow of valuable genetic material nationally and internationally. The current study sought to
BACKGROUND
This 52-week, randomized, double-blind study compared the efficacy and safety of metformin plus pioglitazone with the established combination of metformin plus gliclazide in type 2 diabetes mellitus.
METHODS
Patients with poorly controlled type 2 diabetes (HbA1c > or = 7.5% to < or
BACKGROUND
Non-esterified fatty acids (NEFA) carried on albumin may have a causal role in the development of chronic proteinuria-induced nephropathy. To investigate whether NEFA aggravate renal structural damage, we studied the effects of NEFA addition to delipidated bovine serum albumin (BSA) in