Grape Polyphenols and Metabolic Syndrome
關鍵詞
抽象
描述
Purified polyphenols extracted by table grape can decrease cell proliferation in vitro and exert anti-atherosclerotic and antithrombotic activities, regulating endothelial function. Literature studies have already evaluated the cytostatic and apoptotic effects produced by table grape extracts from different cultivars, demonstrating a different behavior based on extract composition. The beneficial effects of polyphenols have been attributed exclusively to their direct antioxidant action; however, in recent years it has emerged that polyphenols can interact with intracellular signaling mechanisms, modulating the activity of transcription factors involved in cell lipid metabolism. Lipidomic analysis studies the lipids in a "dynamic" way, monitoring the changes in membrane phospholipids content, caused by inflammation, stress, or malnutrition. These changes can also affect the cellular and plasmatic prothrombotic potential, which results altered in metabolic diseases. Recently, alterations in erythrocytes lipidomic profile have been detected in subjects with steatosis. Moreover, in patients with colorectal cancer patients, the presence of metastases at the time of surgery was associated with an altered profile of fatty acids in the membrane of colonic tissue cells.
Moreover, data in literature show how diet and functional foods can modify serum lipid content, in particular, an important role in the onset of dysmetabolic diseases is undoubtedly played by the different fractions of Low-Density Lipoproteins (LDL). The presence of smaller LDL fractions in the serum, such as fraction 3 and fraction 4, has been associated with the onset of cardiovascular disease and myocardial infarctions. Therefore, understanding the molecular mechanisms underlying the effects of nutraceuticals is essential to develop prevention and intervention strategies on subjects at risk for metabolic syndrome.
On this basis, the aim of this study is the evaluation of possible changes happening in lipidomic profile, plasma antioxidant activity and plasma prothrombotic potential induced by a diet enriched with table grape polyphenols in subjects with metabolic syndrome.
日期
| 最後驗證: | 01/31/2020 |
| 首次提交: | 08/05/2019 |
| 提交的預估入學人數: | 08/08/2019 |
| 首次發布: | 08/11/2019 |
| 上次提交的更新: | 02/10/2020 |
| 最近更新發布: | 02/11/2020 |
| 實際學習開始日期: | 09/30/2019 |
| 預計主要完成日期: | 10/31/2020 |
| 預計完成日期: | 10/31/2021 |
狀況或疾病
干預/治療
Dietary Supplement: Diet with table grape
相
手臂組
| 臂 | 干預/治療 |
|---|---|
| Experimental: Diet with table grape Table grape (5g/Kg) administered for four weeks with dietary recommendations. A strict restriction of fruits and the limitation of other foods containing polyphenols will be necessary. | Dietary Supplement: Diet with table grape 5g/Kg of table grape for four weeks with dietary recommendations along with a strict restriction of fruits and limitation of other foods containing polyphenols. |
| No Intervention: Specific dietary advice Dietary recommendations (such as limitation of alcohol, caffeine), and low consumption of fruits. |
資格標準
| 有資格學習的年齡 | 30 Years 至 30 Years |
| 有資格學習的性別 | All |
| 接受健康志願者 | 是 |
| 標準 | Inclusion Criteria: - age > 30 years and <65 years - overweight. Exclusion Criteria: - cardiovascular disease. - stroke - treatment with insulin or oral hypoglycemic drugs - fasting glucose > 126 mg / dl, or casual glycemia > 200 mg / dl - more than 20 g/day of alcohol intake - serious medical conditions that may compromise participation in the trial - subjects following a special diet or involved in a weight-loss program or unable to follow a diet for religious or other reasons. |
結果
主要結果指標
1. Changes in serum concentrations (mg/dL) of cholesterol, triglycerides, glucose [Before the start of the study (time 0), after four weeks (time 1) and after eight weeks (time 2)]
2. Changes in blood concentration of fatty acids (stearic acid, oleic acid, arachidonic acid, eicosaepentanoic acid) expressed as percentage (%) [Before the start of the study (time 0) and after eight weeks (time 2)]
3. Changes in serum concentration of single subfractions of LDL (expressed as mg/dL) [Before the start of the study (time 0) and after eight weeks (time 2)]
次要成果指標
1. Changes in the concentrations of radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) expressed as µM Trolox equivalents/g of dry weight [Before the start of the study (time 0), after four weeks (time 1) and after eight weeks (time 2)]
2. Changes in plasma prothrombotic potential [Before the start of the study (time 0), after four weeks (time 1) and after eight weeks (time 2)]
3. Changes in plasma grape miRNA content [Before the start of the study (time 0) (time 1) and after eight weeks (time 2)]
