Longitudinal Follow-up Study in Two Chinese Hypertension Cohorts
關鍵詞
抽象
描述
This study is a longitudinal cohort follow-up study. The main contents include:
(1) Questionnaire design: the questionnaire is designed according to the research contents and purposes. The standard questionnaires will be used to collect the general information, dietary habits, lifestyle, medical history, and family history etc. (2) Anthropometric measurements: blood pressure, pulse, height, body weight, waist and hip circumference will be acquired by medical practitioners who received professional training based on World Health Organization (WHO) standards, and passed relevant examinations. (3) Blood and urine samples collection: morning fasting blood, nocturia, and 24-hour urine specimen will be collected by medical staffs, and blind tubes will be set up according to 5% of the total samples. Blood biochemistries including serum total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, fasting glucose, creatinine and hsCRP can be measured using automatic biochemical analyzer. The sodium and potassium concentrations in the urine will be measured by flame photometry. The total sodium and potassium excretions in urine in 24h were calculated by multiplying the concentration and 24-h volume of urine. (4) Auxiliary examinations: Auxiliary examinations including carotid intima-media thickness, endothelium-dependent vasodilation (FMD), measurements of brachial-ankle pulse wave velocity (baPWV) and electrocardiographic parameters. The measurement will be done in hospitals by medical practitioners who received professional trainings and passed relevant examinations. (5) Whole-exome sequencing: Genomic DNA will be isolated from blood samples and subjected to exome capture, followed by next-generation sequencing on the Roche NimbleGen system. (6) Whole-genome DNA methylation analysis: levels of DNA methylation will be quantified using the Illumina Infinium HumanMethylation450K Beadchip array (HM450K). (7) Transcriptome sequencing: cDNA libraries will be sequenced via the Illumina HiSeqTM 2500 platform. (8) Data processing and statistics: investigators should input the data to the database, and utilize software such as Access, SPSS, STATA, CASAVA, MutSigCV, and Genome MuSic to analyze. (9) Quality control: ① Questionnaire is strictly designed and amended by epidemiologists and clinical experts. ② A standardized "Investigator Handbook" will be stipulated according to the guidelines. ③ Staff members are required to do a rigorous training and pass the exam. ④ The investigation process will be strictly supervised by the principal investigator. ⑤ Data entry uses the parallel double-entry method. ⑥ During laboratory testing, blank control and blind detection are applied to ensure the quality. ⑦ Data analysis is performed by 2-3 postgraduates, inconsistent data need to be double-checked.
日期
| 最後驗證: | 05/31/2020 |
| 首次提交: | 06/20/2020 |
| 提交的預估入學人數: | 07/12/2020 |
| 首次發布: | 07/14/2020 |
| 上次提交的更新: | 07/12/2020 |
| 最近更新發布: | 07/14/2020 |
| 實際學習開始日期: | 08/31/2020 |
| 預計主要完成日期: | 11/30/2021 |
| 預計完成日期: | 11/30/2022 |
狀況或疾病
干預/治療
Other: Incidence and risk factors of hypertension
相
手臂組
| 臂 | 干預/治療 |
|---|---|
| Hanzhong adolescent hypertension cohort A total of 4623 adolescents aged 6-15 years old in Hanzhong rural areas was recruited in 1987. During the follow-up period, the information about the incidence and risk factors of hypertension will be collected. | |
| Mei county adult salt-sensitive hypertension cohort A total of 675 individuals from 126 families were recruited in this family-based dietary intervention study. A community-based BP screening was conducted among adults aged 18-60 years in the study villages. The probands who had a mean systolic BP (SBP) between 130-160 mmHg and/or a diastolic BP (DBP) between 85-100 mmHg and no use of antihypertensive medications and their parents, siblings, spouses, and offspring were recruited in this study. During the follow-up period, the information about the incidence and risk factors of hypertension will be collected. |
資格標準
| 有資格學習的年齡 | 40 Years 至 40 Years |
| 有資格學習的性別 | All |
| 取樣方式 | Probability Sample |
| 接受健康志願者 | 是 |
| 標準 | Inclusion Criteria: 1. Hanzhong cohort of Adolescent Hypertension Study: • adolescents aged 6-15 years old in over 20 schools of three towns (Qili, Laojun and Shayan) in Hanzhong, Shaanxi, China. 2. Mei county cohort of adult salt-sensitive hypertension study: - Han individuals in rural northern China - adults aged 18-60 years - individuals who had a mean systolic BP (SBP) between 130-160 mmHg and/or a diastolic BP (DBP) between 85-100 mmHg and no use of antihypertensive medications were identified as the proband - the probands'parents, siblings, spouses, and offspring were recruited for the study. Exclusion Criteria: - Secondary hypertension - a history of severe cardiovascular disease - chronic kidney disease or liver disease - unable to complete the examination - unable/refuse to sign the informed consent form |
結果
主要結果指標
1. blood pressure value(mmHg) [First day of the three-day follow-up period]
次要成果指標
1. Left ventricular hypertrophy (g/m^2) [During the three-day follow-up period]
2. Increased arterial stiffness (mm/s) [During the three-day follow-up period]
3. Carotid artery wall thickening (mm) [During the three-day follow-up period]
4. Microalbuminuria (mg/24h) [Second day of the three-day follow-up period]
5. whole exome sequencing [the whole year after the follow-up period]
6. whole genome DNA methylation detection [the whole year after the follow-up period]
7. whole-genome transcriptome sequencing [the whole year after the follow-up period]
