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Degeneration is the process whereby Clostridium acetobutylicum ATCC 824 loses the capacity to produce acetone and butanol after repeated vegetative transfers or in continuous culture. Two degenerate mutants (M5 and DG1) of C. acetobutylicum ATCC 824 do not contain the four genes (ctfA, ctfB, adc,
Strain degeneration in solventogenic clostridia is a known problem in the technical acetone-butanol fermentation bioprocess, especially in the continuous process mode. Clostridial strain degeneration was studied by Fourier transform infrared (FT-IR) spectroscopy of the bacterial cells. Degenerative
Topical application of 5 microgram of a juvenile hormone analogue (JHA), ZR-615, to female callow adults of Ips paraconfusus induced degeneration of the dorsoventral flight muscles. Within 24 h after JHA-treatment the diameter of the myofibrils was reduced to almost half due to the lysis of the
Time-resolved degenerate four-wave mixing experiments on a colloidal solution of 50-nm gold particles in acetone, using a high-repetition-rate laser source, are presented. The signal is found to depend strongly on the repetition rate of the incident radiation and above a frequency of 300 kHz is
The hindered rotations of acetone were studied density functional theory (B3LYP) and second order Møller-Plesset approaches using 6-31G** and 6-311G** basis sets. One of the CH(3) groups of acetone with fixed heavy atoms was rotated from 0.0 to 120 degrees, and CCH angles were scanned from 90.3 to
The acetone-butanol-ethanol (ABE) fermentation industry in China was started in the early 1950s in Shanghai and expanded rapidly thereafter. At its peak, there were about 30 plants all over the country and the total annual production of solvents reached 170,000 tons. This large enterprise was
A continuous acetone-butanol-ethanol (ABE) production system with high cell density obtained by cell-recycling of Clostridium saccharoperbutylacetonicum N1-4 has been studied. In conventional continuous culture of ABE without cell-recycling, the cell concentration was below 5.2 g l(-1) and the
Fresh beef steaks were treated with two concentrations of a methanol-acetone (MA) extract ( Streptococcus thermophilus -fermented skim milk) for color stability, pH change, and microbial growth at storage temperatures of 0 and 10°C. Beef color was measured by a color difference meter (Gardner) and a
We examined the bladder function of cystitis models induced by intravesical acetone instillation in urethane-anesthetized rats. Acetone (0.35 ml) at 10, 30, or 50% concentration or deionized water (sham-treatment) was instilled into the bladder via the cannula which was inserted into the lumen.
Lipid peroxidation (LPO) is the oxidative deterioration of polyunsaturated fatty acids (PUFA) with the production of lipid hydroperoxides, cyclic peroxides, cyclic endoperoxides, and finally fragmentation to ketones and aldehydes (including malonaldehyde, MDA). Estimation of LPO through MDA
Acetone-butanol-ethanol (ABE) fermentation was successfully carried out in an immobilized cell trickle bed reactor. The reactor was composed of two serial columns packed with Clostridium acetobutylicum ATCC 824 entrapped on the surface of natural sponge segments at a cell loading in the range of
Konzo is a neurotoxic motor disease caused by excess consumption of insufficiently processed cassava. Cassava contains the cyanogenic glucoside linamarin, but konzo does not present the known pathological effects of cyanide. We hypothesized that the aglycone of linamarin, acetone cyanohydrin, may be
Following section of the optic nerve, degenerating retinal terminals reveal an accumulation of neurofilaments (neurofilamentous hypertrophy) as demonstrated by silver impregnation techniques or electron microscopy. The present study examined degenerating retinal terminals by means of
In two separate studies with exposure duration 9 weeks or 4 weeks, male Wistar rats were dosed with di(2-ethylhexyl)phthalate (DEHP) by gavage and exposed to drinking water with or without acetone (0.5% wt/v in the 9-week study, 1.0% wt/v in the 4-week study). In the 9-week study the doses of DEHP
Acetone extract of whole fruit powder of M. charantia (bitter gourd) in doses 25, 50 and 75 mg/100 g body weight lowered the blood glucose from 13.30 to 50% after 8 to 30 days treatment in alloxan diabetic albino rats, confirming antihyperglycemic effect of this plant in diabetic animals and humans.