polyprenol/spinacia oleracea

Polyisoprenoid alcohols occurring in spinach leaves were analyzed by a two-plate TLC method. Z,E-mixed polyprenols (C(55-60)), glycinoprenols (C(50-55)), and solanesol (C(45)) were mainly found in chloroplasts, whereas dolichols (C(70-80)) were mainly found in microsomes. Analysis of enzymatic

Isolated chloroplasts from spinach leaf cells, chloroplast subfractions, and a cell-free system of the cyanobacterium Synechococcus CCAP 6312 incorporated [1-(14)C]isopentenyl pyrophosphate in high yields into prenyl lipids. Products were polyprenols (C20, C45) chlorophylls, quinoid compounds, and

The localization of ubiquinone (UQ) and plastoquinone (PQ) biosynthesis in subfractions isolated from spinach leaves has been studied. UQ-9 and UQ-10 were found mainly in mitochondria, whereas PQ was enriched in chloroplasts, but also found in Golgi membranes. alpha-Unsaturated polyprenol-11 was

In this paper evidence is presented for the occurrence of prenylated proteins in plants. When spinach leaves were incubated in the presence of [3H]mevalonate non-extractable lipids were found in the protein fraction after extraction with organic solvents. Alkaline hydrolysis liberated phytol,