sphaerophysa kotschyana/flavonoid

Two new flavonoids, named sphaerophyside SA and sphaerophyside SB, together with 15 known flavonoids were isolated from the ethanolic extract of the seeds of Sphaerophysa salsula (Pall.) DC. The structures of the new compounds were elucidated mainly on the basis of the 1D and 2D NMR data.

An offline preparative two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography coupled with hydrophilic interaction solid-phase extraction method was developed for the preparative isolation of flavonoid glycosides from a crude sample of Sphaerophysa

Flavonoid glycosides exist widely in medicine herbs and often used as nutraceuticals because of their excellent bioactivity and low toxicity. For accurate quality control and bioactivity assessment of Sphaerophysa salsula, a rapid and productive method to isolate flavonoid glycosides is needed.

OBJECTIVE To study the chemical constituents of Sphaerophysa salsula (Pall.) DC. METHODS Compounds were isolated by repeated chromatography, on silica gel and Sephadex LH-20 columns and identified by spectroscopic data (ESI-MS, 1H and 13C NMR, HSQC, HMBC). RESULTS Eight compounds have been isolated