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Avoiding false discovery in biomarker research.
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Human tyrosine-protein phosphatase non-receptor type substrate 1α (SIRPA) is a surface marker identified in cardiomyocytes differentiated from human embryonic stem cells. Our objective was to determine if circulating SIRPA levels can serve as a biomarker of cardiac injury in children undergoing open
[False test of albumin in urine after intravenous urography].
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False myths and legends in laboratory diagnostics.
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Remarkable advances in understanding human biology in health and disease, propelled by technological innovations, have contributed to an increase in the number and quality of diagnostic tests. This evolving scenario has been accompanied by the proliferation of false myths and legends in laboratory
Avoiding false-positive joint scans by the use of labeled albumins.
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[False-positive reaction of urinary ketone bodies caused by bucillamine].
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In many patients taking bucillamine, an anti-rheumatic drug, urinary examinations show false-positive findings for ketone on urine dipsticks, because of the SH radicals in bucillamine. Our group examined the frequency of false-positive ketone testing and the causative factors in 179 outpatients
Serum albumin leads to false-positive results in the XTT and the MTT assay.
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Tetrazolium salts like 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) or sodium 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) that form formazans after reduction are widely used to investigate cell viability. Besides cellular enzymes, some
False positive immunoassay results for cholecystokinin with a phosphate buffer containing bovine serum albumin.
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[False-negative antiglobulin-(Coombs-) test caused by anticomplement bovine albumin batches in the transfusion preparation].
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Different brands of bovine albumins were tested as reagent in the second step of the so called 3-stage-screening test for irregular blood group antibodies. In 3 of 9 brands of bovine albumin there was anticomplementary activity which was the cause of diminished or negative reactions in the third
Development of peptide receptor binding assays: methods to avoid false negatives.
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Selection of appropriate ligand receptor binding assay conditions is critical for peptides, where the possibility of obtaining false negative results is pertinent due to their inherent adsorption and instability characteristics, as well as high response-sensitivity to operational conditions. The aim
Factors affecting false-negative breast sentinel node biopsy in Chinese patients.
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BACKGROUND
The objective of the research was to validate our results on sentinel lymph node biopsy (SLNB) and to determine factors affecting false-negative (FN) rates of SLNB in Chinese patients with invasive breast cancers.
METHODS
A retrospective study of patients with clinically node-negative
Naturally occurring anti-albumin antibodies are responsible for false positivity in diagnosis of autoimmune premature ovarian failure.
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Autoimmunity is a well-established causative factor of premature ovarian failure (POF), and evidence for the same has been well reported in the literature. Detection of specific autoantibodies remains the most practical clinical research marker of any autoimmune disease. Variation in efficiency and
Elimination of false-positive results in a luminex assay for pneumococcal antibodies.
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In our 14-valent Luminex assay for pneumococcal antibodies, we identified two groups of sera that caused false-positive results. The first group bound nonspecifically to the Luminex microspheres. The second group reacted specifically with bovine serum albumin (BSA). We describe here methods that
Escherichia coli O157 serology: false-positive ELISA results caused by human antibodies binding to bovine serum albumin.
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An analysis of farm workers and rural dwellers for serum antibodies to the lipopolysaccharide (LPS) of Escherichia coli O157 detected sera with antibodies binding to bovine serum albumin (BSA) by ELISA. These antibodies were not specific for BSA when examined by immunoblotting, and the ELISA values
Albumin autoagglutinating phenomenon as a factor contributing to false positive reactions when typing with rapid slide-test reagents.
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A case of albumin autoagglutination has illustrated the point that this phenomenon may be a further source of false positive reactions when typing with rapid slide-test reagents. As agglutination only occurred with the bovine albumin solution and slide-test reagents from a single manufacturer, it is
Presumptive false positive serologic reactions for syphilis in Central America. III. Relation to serum protein, albumin, and globulin.
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