Phytochemical investigations on Iris germanica.

Phytochemical investigations on the methanol extract of Iris germanica resulted in the isolation of a new benzene derivative, 2'-methyl-6'-hydroxy cyclohexenyl-3-methyl-1-acetophenone ether (1). Further, another known benzene derivative, isopenol (2), also afforded two known isoflavones, irisolone (3) and irisolidone (4). The structure of the new compound was determined on the basis of spectroscopic data, including 2D-NMR experiments, while the known compounds were identified on the basis of their spectral data and existing literature evidence. The comparison of the spectral data of the irisolidone (3) with that reported for the molecule led us to revise some of the reported 1H-NMR chemical shift assignments.