Subjects with sensitive skin, diagnosed by the lactic acid stinging test. Skin biopsy Oral mucosa specimen

Subjects without sensitive skin, determined by a negative lactic acid stinging test Skin biopsy Oral mucosa specimen

Sensitive Skin

Two skin biopsies will be taken with a 3 mm punch in the retroauricular area. The procedure will be done by an investigator, under aseptic and antiseptic conditions and under local anesthesia with lidocaine and epinephrine. The incision will be sutured with 6-0 Nylon, and the stitches will be removed after 5 days. One biopsy will be processed for immunohistochemistry, the other for RNA extraction and analysis.

Skin biopsy

The sample for keratinocytes from oral mucosa will be taken with a Foam knife, which is a non-invasive procedure. It does not need anesthesia, and it does not leave scars. The procedure consists in gently brush the oral mucosa with the knife five times, and the material that will be obtained will be fixed in a PBS solution for RNA analysis.

Oral mucosa specimen

Dermatology Department. Hospital Central "Dr. Ignacio Morones Prieto"

TRPV Expression in Subjects With Sensitive Skin

Distribution and Expression of Non-neuronal Transient Receptor Potential (TRPV) Ion Channels in Sensitive Skin Syndrome.

Determine the expression of TRPV1 in patients with sensitive skin

Correlate the expression of TRVP1 with the presence of sensitive skin syndrome

Identify variations in the expression of TRPV1 according to skin phototype

Correlate the expression of TRPV1 with the transepidermal water loss as an indirect measure of barrier function.

Quantify the expression of mRNA of TRPV1 in epidermal keratinocytes obtained by skin biopsies through RT-PCR

Quantify the expression of TRPV1 in epidermal keratinocytes obtained by skin biopsies through immunohistochemistry

Quantify the expression of mRNA of TRPV1 in epidermal keratinocytes obtained from oral mucosa through RT-PCR

Quantify the expression of TRPV1 in epidermal keratinocytes obtained from oral mucosa through immunohistochemistry.

Juan Pablo Castanedo-Cazares

Dermatology Research Director

Universidad Autonoma de San Luis Potosí

Hospital Central "Dr. Ignacio Morones Prieto"

Children with asthma treated with montelukast and budesonide.

Children with asthma treated with placebo tablet and budesonide.

Bronchial Asthma

Montelukast sodium.

Placebo tablet and budesonide

Department of Pediatrics and Allergy, Medical University of Lodz, N. Copernicus Hospital

Effect of Montelukast in Asthma in Children

The Effect of Montelukast on Anti-inflammatory Treatment and Asthma Exacerbation Prevention in Children Sensitive to Dust Mites.

steroid doses sufficient to control asthma symptoms
medium steroid dose/day times 6 month
number of asthma exacerbations in 9 month period
maximum fall of FEV1 in exercise induced bronchoconstriction test
asthma symptoms scale and lung function
number of patients with positive airway hyperresponsiveness test

Iwona Stelmach

MD, PhD, Prof

Medical Universtity of Lodz

patient without CAD, not on aspirin and without history of hypersensitivity

patient with CAD on aspirin, with a history of hypersensitivity

patient with CAD on aspirin, without history of hypersensitivity

leucotriene/inflammation

TRPV Expression in Subjects With Sensitive Skin

Effect of Montelukast in Asthma in Children

Physiopathology of Rapid Aspirin Desensitization

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